Acoustic emission (AE) diagnostics was carried out during tensile testing of 20KhN2MA steel samples to study the loss of ductility after the impact. We used V-notched samples (3.3 mm in depth) with overall dimensions of 300 × 20 × 6 mm. The impact in the concentrator zone caused the depletion of the plasticity of the material, which was accompanied by a decrease in the partial fraction of ductile fracture and an increase in brittle fracture. The test samples were divided into six batches. The samples of the first batch were not subjected to the impact. In the second batch, the impact energy was 50 J, in the third — 75 J, in the fourth — 100 J, in the fifth — 125 J and in the sixth — 150 J. The rupture tests were carried out at room temperature and at a speed of the movable traverse of 1 mm/min. The kinetics of damage in the notch zone during loading was monitored using the acoustic emission (AE) method and video recording. Processes of brittle and ductile (caused by cleavage and shear, respectively) destruction of the crystal lattice of a metal differ primarily in the speed and duration of stress waves. To separate AE pulses generated by these processes, spectrograms of time-frequency transformations and waveforms were analyzed. Pulse selection was carried out using a complex parameter reflecting the steepness of the amplitude drop at the phase of signal attenuation. Boundary values were determined that allow separation of the recorded pulses into flows caused by ductile and brittle structural damage to structural steels. It is shown that manifestation of the effect of impact on the exhaustion of the plastic properties of steel 20KhN2MA becomes noticeable when the level of specific work exceeds 50 J/cm2. Moreover, with an increase in the specific work up to 150 J/cm2, the weight content of location pulses characterizing the kinetics of brittle destruction of structural bonds increased by 3 – 4 times, relative to that recorded for the samples without impact. This result correlates with the duration of the rupture test of the samples, which was reduced by three times when the level of the specific work increased to 150 J/cm2