General, rapid, and transcription-dependent fragmentation of nucleolar antigens in <i>S. cerevisiae</i> mRNA export mutants

Thomsen, Rie; Saguez, Cyril; Nasser, Tommy; Jensen, Torben Heick

doi:10.1261/rna.718708

Cited by 17 publications

(17 citation statements)

References 58 publications

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“…Nup59p is required for mRNA export after severe heat shock but is dispensable for peripheral localization of INO1 after inositol starvation (Thomsen et al 2008;Ahmed et al 2010;Light et al 2010). We found that a nup59D had no defect in stress-responsive transcriptional memory at TSA2, unlike nup42D cells ( Figure 5); a similar result was found for the related nup60D mutant (Thomsen et al 2008) (not shown). This strongly argues against a general defect in mRNA export as the cause of the transcriptional difference.…”

Section: Nuclear Pore Component Nup42p Is Required For the Faster Gensupporting

confidence: 81%

Cellular Memory of Acquired Stress Resistance inSaccharomyces cerevisiae

et al. 2012

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Cellular memory of past experiences has been observed in several organisms and across a variety of experiences, including bacteria "remembering" prior nutritional status and amoeba "learning" to anticipate future environmental conditions. Here, we show that Saccharomyces cerevisiae maintains a multifaceted memory of prior stress exposure. We previously demonstrated that yeast cells exposed to a mild dose of salt acquire subsequent tolerance to severe doses of H 2 O 2 . We set out to characterize the retention of acquired tolerance and in the process uncovered two distinct aspects of cellular memory. First, we found that H 2 O 2 resistance persisted for four to five generations after cells were removed from the prior salt treatment and was transmitted to daughter cells that never directly experienced the pretreatment. Maintenance of this memory did not require nascent protein synthesis after the initial salt pretreatment, but rather required long-lived cytosolic catalase Ctt1p that was synthesized during salt exposure and then distributed to daughter cells during subsequent cell divisions. In addition to and separable from the memory of H 2 O 2 resistance, these cells also displayed a faster gene-expression response to subsequent stress at .1000 genes, representing transcriptional memory. The faster gene-expression response requires the nuclear pore component Nup42p and serves an important function by facilitating faster reacquisition of H 2 O 2 tolerance after a second cycle of salt exposure. Memory of prior stress exposure likely provides a significant advantage to microbial populations living in ever-changing environments. N ATURAL environments are complex and often vary significantly in space and time, posing challenges for the organisms living within them. Single-cell organisms are particularly vulnerable, since variation in external conditions can directly impact internal homeostasis. Therefore, preparing for environmental change after early signs of fluctuation would present a significant advantage for cells growing in the wild. Indeed, many organisms can become tolerant to severe stress after an initial mild pretreatment with the same or a different stressor. This response, termed "acquired stress resistance," has been observed in microbes such as bacteria and yeast as well as in multicellular organisms including worms, plants, mammals, and even humans (Lu et al. 1993;Davies et al. 1995;Lewis et al. 1995;Lou and Yousef 1997;Swan and Watson 1999;Chi and Arneborg 2000;Schenk et al. 2000;Durrant and Dong 2004;Kandror et al. 2004;Scholz et al. 2005;Hecker et al. 2007;Kensler et al. 2007;Matsumoto et al. 2007). The conservation of this response suggests that it plays an important role in surviving environmental stress in diverse species.We previously conducted a systematic analysis of acquired stress resistance in Saccharomyces cerevisiae and found that the response is common, but not universal, for all pairs of stress treatments (Berry and Gasch 2008). The level and duration of mild-stress pretreatme...

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Section: Nuclear Pore Component Nup42p Is Required For the Faster Gensupporting

confidence: 81%

Cellular Memory of Acquired Stress Resistance inSaccharomyces cerevisiae

et al. 2012

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“…Further studies are required to resolve the identity of the polyA( þ ) RNA accumulating in the nucleolar aggregates, as polyA( þ ) RNA may represent either nascent mRNA exported via the CRM1 route or RNA destined for degradation by polyA( þ ) tag. However, the finding that ubiquitin is rate limiting for the aggregate formation suggests that ubiquitin-mediated licensing is required for RNA surveillance and export also in mammalian cells, as previously described in yeast (Thomsen et al, 2008;Houseley and Tollervey, 2009).…”

Section: Nucleolar Aggregates L Latonen Et Almentioning

confidence: 53%

Proteasome inhibitors induce nucleolar aggregation of proteasome target proteins and polyadenylated RNA by altering ubiquitin availability

et al. 2010

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“…We also speculate that some miRNAs could combine with target messenger RNAs in the nucleolus to be exported as ''presuppressed'' mRNAs, a somatic cell analogy with masked maternal mRNAs in oocytes. Consistent with this idea, there is evidence that the nucleolus is involved in mRNA nucleocytoplasmic transport (Schneiter et al 1995;Thomsen et al 2008), and some mRNAs have been shown to associate with the nucleolus (John et al 1977;Bond and Wold 1993;Bártová et al 2008;Kim et al 2009). …”

Section: Discussionmentioning

confidence: 80%

MicroRNAs with a nucleolar location

Politz¹,

Hogan²,

Pederson³

2009

RNA

169

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There is increasing evidence that noncoding RNAs play a functional role in the nucleus. We previously reported that the microRNA (miRNA), miR-206, is concentrated in the nucleolus of rat myoblasts, as well as in the cytoplasm as expected. Here we have extended this finding. We show by cell/nuclear fractionation followed by microarray analysis that a number of miRNAs can be detected within the nucleolus of rat myoblasts, some of which are significantly concentrated there. Pronounced nucleolar localization is a specific phenomenon since other miRNAs are present at only very low levels in the nucleolus and occur at much higher levels in the nucleoplasm and/or the cytoplasm. We have further characterized a subset of these miRNAs using RT-qPCR and in situ hybridization, and the results suggest that some miRNAs are present in the nucleolus in precursor form while others are present as mature species. Furthermore, we have found that these miRNAs are clustered in specific sites within the nucleolus that correspond to the classical granular component. One of these miRNAs is completely homologous to a portion of a snoRNA, suggesting that it may be processed from it. In contrast, the other nucleolar-concentrated miRNAs do not show homology with any annotated rat snoRNAs and thus appear to be present in the nucleolus for other reasons, such as modification/processing, or to play roles in the late stages of ribosome biosynthesis or in nonribosomal functions that have recently been ascribed to the granular component of the nucleolus.

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General, rapid, and transcription-dependent fragmentation of nucleolar antigens in S. cerevisiae mRNA export mutants

Cited by 17 publications

References 58 publications

Cellular Memory of Acquired Stress Resistance inSaccharomyces cerevisiae

Cellular Memory of Acquired Stress Resistance inSaccharomyces cerevisiae

Proteasome inhibitors induce nucleolar aggregation of proteasome target proteins and polyadenylated RNA by altering ubiquitin availability

MicroRNAs with a nucleolar location

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