General scheme of analysis of phenolic compound in plant extracts by reversed-phase high-performance liquid chromatography

Villeneuve, F.; Abravanel, G.; Moutounet, Michel; Alibert, Gilbert

doi:10.1016/s0021-9673(00)81787-2

Cited by 43 publications

(10 citation statements)

References 11 publications

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“…The ethyl acetate extract was diluted to 2.5 L with methanol. A 1.0-mL aliquot was spiked the 18.9 mmol of resorcinol as an internal standard, and phenolic glycosides (salicin, picien, triandrin) were quantified by high pressure liquid chromatography (HPLC) (Villeneuve et al 1982) using a reverse phase (C 18 ) analytical column and watermethanol gradient (flow rate 1 mL!min). Detection was accomplished with an ultraviolet detector at 250 nm.…”

Section: Chemical Analysis and Bomb Calorimetry Of Samplesmentioning

confidence: 99%

Interactions of Snowshoe Hare and Feltleaf Willow in Alaska

Bryant¹,

Wieland²,

Clausen³

et al. 1985

Ecology

103

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Evidence from experimental feeding trials and chemical analysis showed that, when severe winter browsing of adult—form Alaska feltleaf willow (Salix alaxensis) causes it to revert to juvenile—form stump sprouts, the twigs are unpalatable and of lower nutritional quality to the hares than are twigs of adult—form S. alaxensis plants. Further feeding choice trials demonstrated that the palatability of winter—dormant S. alaxensis to snowshoe hares is a function of the age of the sprout and its chemistry rather than the accessibility (height) or morphology of the twigs. The low nutritional quality of juvenile sprouts is partially caused by decreased dry matter and nitrogen digestibility, which may be related to increased lignin and phenolic content of the twigs. This browsing—induced resistance to future winter browsing by hares relaxes within 3 yr and within the height range of twigs normally available to snowshoe hares in winter. These results are compatible with hypothesis that the inducation and relaxation of resistance to browsing in the preferred winter food of snowshoe hares is a cause of the well—known 10—yr snowshoe hare cycle.

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Section: Chemical Analysis and Bomb Calorimetry Of Samplesmentioning

confidence: 99%

Interactions of Snowshoe Hare and Feltleaf Willow in Alaska

Bryant¹,

Wieland²,

Clausen³

et al. 1985

Ecology

103

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“…This latter compound remains unchanged in the modified method and corresponds to the quantity of OHCA found with the classical method. Although free coumarin is the prevailing product extracted with the Villeneuve and Abravanel (1982) method, this is not the case with the modified method. As already demonstrated by Haskin and Gorz (1961), coumarin is not present in the free form inside the plant but rather in a glycosidic form.…”

Section: Resultsmentioning

confidence: 96%

“…The highest coumarin concentrations were given by the water extraction method. The Villeneuve and Abravanel (1982) method (extraction with EtOH) also extracted large quantities of two molecules, namely coumarin and trans 0-hydroxycinnamic acid (OHCA). This method gave the highest coumarin concentration compared with the other six methods and trans OHCA could only be extracted with this reference method.…”

Section: Resultsmentioning

confidence: 99%

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Extraction of coumarins from plant material (Leguminosae)

Bourgaud

Poutaraud

Guckert

1994

Phytochemical Analysis

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Seven different methods for the extraction of coumarin and corresponding glycosides from Melilotus officinalis L. and furanocoumarins (psoralen and angelicin) from Psoruleu cinerea Lindl. were compared. In the case of coumarins, extraction with polar solvents (water, ethanol, methanol) was shown to be the most efficient: water extraction gave the highest total coumarin concentration, especially for the major component fl-D-ghKosyl cis 0-hydroxycinnamic acid. Free coumarin could also be recovered in large quantity when the extraction was performed with water at room temperature instead of at 100°C. Ethyl acetate, diethyl ether and chloroform extracted the free coumarin poorly and the glycosides hardly at all. In the case of furanocoumarins, the best results were obtained with boiling methanol using either Soxhlet extraction or refluxed solvent. Other extraction methods involving water, methanol at room temperature, ethyl acetate or chloroform gave essentially similar results. Diethyl ether was the least satisfactory in terms of furanocoumarin concentration, whilst water extraction of furanocoumarins is of particular interest because the extracted quantities can be easily correlated with the methanol method.

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“…High pressure liquid Chromatography (HPLC) is more promising because it allows high resolution and a rapid and reproducible determination even of trace compounds. Villeneuve et al (1982) designed a general scheme for the analysis of plant phenolics, including coumarins, using reverse-phase (RP)-HPLC. Guo and Zhang (1983b) described the quantitative RP-HPLC determination of esculin, esculetin and fraxin in Fraxinus clrinensis.…”

Section: Introductionmentioning

confidence: 99%

Reverse‐phase high pressure liquid chromatographic analysis of hydroxycoumarins in plant extracts. Quantitative determination of hydroxycoumarins in Fraxinus ornus

Nykolov

Iossifova

Vassileva

et al. 1993

Phytochemical Analysis

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A reverse‐phase high pressure liquid chromatographic determination of 11 hydroxycoumarins in plant extracts is described, which is applicable to the quantitative analysis of these compounds for industrial purposes. Fraxinus ornus bark from various regions in Bulgaria showed differences both in the total coumarin content and in the relative proportions of each component. The occurrence of 7‐methylesculin and 6,7,8‐trihydroxycoumarin in the family Oleaceae is reported for the first time.

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General scheme of analysis of phenolic compound in plant extracts by reversed-phase high-performance liquid chromatography

Cited by 43 publications

References 11 publications

Interactions of Snowshoe Hare and Feltleaf Willow in Alaska

Interactions of Snowshoe Hare and Feltleaf Willow in Alaska

Extraction of coumarins from plant material (Leguminosae)

Reverse‐phase high pressure liquid chromatographic analysis of hydroxycoumarins in plant extracts. Quantitative determination of hydroxycoumarins in Fraxinus ornus

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