Generalized multiparticle Mie modeling of light scattering by cells

Abstract

The development of nanobiology requires a fundamental understanding of the interaction features between light and cells as well as cells containing nanoparticles. In this study, the generalized multiparticle Mie (GMM) theory was employed to calculate the scattering properties of cells under refractive index matching conditions. The angular distribution of scattered light is statistically averaged to obtain a good fit for the experimental results. Based on a simplified cell model, the variabilities between the scattered light pattern of normal cells and that of cancerous cells were examined. The results indicate that the small angle scattering is sensitive to the organelle distribution, which could be applied in the diagnostics of cancerous cells. Finally, the effects of cellular uptake of nanoparticles on the scattering pattern was also investigated.

Substrate for Surface-Enhanced Raman Spectroscopy Formed by Gold Nanoparticles Buried in Poly(methyl methacrylate)

“…GMM simulations for the SERS EF were done in order to have an insight about the effect of NPs’ size and about the distance between them for SERS enhancement. This method uses an extension of Mie’s theory for the case of multiple particles, being a semi-analytic solution for the scattering of light by an arbitrary set of isolated spheres …”

“…This method uses an extension of Mie's theory for the case of multiple particles, being a semi-analytic solution for the scattering of light by an arbitrary set of isolated spheres. 36 The excitation radiation for EF calculation was 633 nm, and the dielectric medium containing the particles was water in order to simplify the simulation (refractive index of the dielectric medium, n = 1.33). The geometric parameters used were based on the resulting NP geometry (NPs' average diameter and distance between NPs), as will be seen.…”

Rare-Earth Elements

“…In the latter case, Mie theory has been applied to determine if scattered light from appropriately treated tissue can be used to diagnose cancerous from healthy cells [25,26]. Mie scattering is used in particle size determination for particles in non-absorbing media [27], in the determination of the oil concentration in polluted water [28], in parasitology [29], and in the design of metamaterials [30].…”

Maxwell's equations based 3D model of light scattering in the retina

“…However, Mie's solution only describes the scattering patterns for a single homogeneous sphere, which limits its application to scatterers of different shapes and aggregates of scatterers. The Generalized Multiparticle Mie (GMM) introduced by Xu is an extension of Mie's solution to multiple scattering (aggregates of spheres) [10], that models more accurately light scattering from biological tissues, as assessed by Wang et al [11]. Nevertheless, GMM is also restricted to spherical structures.…”