“…In contrast, eDNA metabarcoding can be used to characterize entire species assemblages (Cilleros et al, ; Deiner et al, ; DiBattista et al, ; Yamamoto et al, ). Environmental DNA metabarcoding can be used to simultaneously determine species distributions, ecologically important patterns of species diversity, and detect novel species (Blackman et al, ; Elbrecht, Vamos, Meissner, Aroviita, & Leese, ; Harper et al, ). Metabarcoding surveys are able to provide comprehensive presence/absence data, although sampling, laboratory, and analytical protocols can influence biodiversity estimates (Alberdi et al, ; Deiner, Walser, Mächler, & Altermatt, ).…”