Generating hESCs with reduced immunogenicity by disrupting TAP1 or TAPBP

Cui, Di; Wang, Jinping; Zeng, Yelin; Rao, Lingjun; Chen, Haide; Li, Wenling; Li, Yang; Li, Hui; Cui, Chun; Xiao, Lei

doi:10.1080/09168451.2016.1165601

Cited by 20 publications

(7 citation statements)

References 45 publications

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“…Our results show also the downregulation of MAPK9 , encoding for JNK2 protein, the absence of which was associated with the hyperproliferation of the CD8+ T cells [ 57 ]. Moreover, the result is in line with the upregulation of TAPBP , encoding for tapasin protein, required to increase the affinity with MHC class I for the activation of the immunogenicity [ 58 ]. It is noteworthy that our results show that the genes belonging to the pathways that we took into consideration for the immune system are not statistically differentially expressed in frontal and in parietal cortex of MS patients compared to healthy controls.…”

Section: Discussionmentioning

confidence: 94%

Could the Heat Shock Proteins 70 Family Members Exacerbate the Immune Response in Multiple Sclerosis? An in Silico Study

Chiricosta

Bramantı

Mazzon

2020

Genes

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Multiple sclerosis (MS) is a chronic autoimmune demyelinating disease of the central nervous system. It represents one of the main causes of neurological disability in young people. In MS, the autoimmune response is directed against myelin antigens but other possible bio-molecular markers are investigated. The aim of this work was, through an in silico study, the evaluation of the transcriptional modifications between healthy subjects and MS patients in six brain areas (corpus callosum, hippocampus, internal capsule, optic chiasm, frontal and parietal cortex) in order to identify genes representative of the disease. Our results show the upregulation of the Heat Shock Proteins (HSPs) HSPA1A, HSPA1B, HSPA7, HSPA6, HSPH1 and HSPA4L of the HSP70 family, among which HSPA1A and HSPA1B are upregulated in all the brain areas. HSP70s are molecular chaperones indispensable for protein folding, recently associated with immune system maintenance. The little overexpression of the HSPs protects the cells from stress but extreme upregulation can contribute to the MS pathogenesis. We also investigated the genes involved in the immune system that result in overall upregulation in the corpus callosum, hippocampus, internal capsule, optic chiasm and are absent in the cortex. Interestingly, the genes of the immune system and the HSP70s have comparable levels of expression.

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Section: Discussionmentioning

confidence: 94%

Could the Heat Shock Proteins 70 Family Members Exacerbate the Immune Response in Multiple Sclerosis? An in Silico Study

Chiricosta

Bramantı

Mazzon

2020

Genes

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“…Remarkably, Cui et al found that the expression levels of HLA-ABC were upregulated even in the TAPBP knock-out cells by the interferon treatment, and immune rejection was reduced in TAPBP-deficient hESC line. Potent inflammatory molecules such as eicosanoids are able to upregulate TAPBP [41,42].…”

Section: Discussionmentioning

confidence: 99%

The Epigenetic Overlap between Obesity and Mood Disorders: A Systematic Review

Gharipour

Barekatain

Sung

et al. 2020

IJMS

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(1) Background: Obesity and mood disorders are considered as the most prevalent morbidities in many countries. We suppose that epigenetic mechanisms may induce higher rates of obesity in subjects who suffer from mood disorders. In this systematic review, we focused on the potential roles of DNA methylation on mood disorders and obesity development. (2) Methods: This systematic review was conducted in accordance with the PRISMA statement and registered in Prospero. A systematic search was conducted in MEDLINE, Scopus, Web of Science, Cochrane Central database, EMBASE, and CINHAL. We also conducted a Grey literature search, such as Google Scholar. (3) Results: After deduplication, we identified 198 potentially related citations. Finally, ten unique studies met our inclusion criteria. We have found three overlap genes that show significant DNA methylation changes, both in obesity and depression. Pathway analysis interaction for TAPBP, BDNF, and SORBS2 confirmed the relation of these genes in both obesity and mood disorders. (4) Conclusions: While mechanisms linking both obesity and mood disorders to epigenetic response are still unknown, we have already known chronic inflammation induces a novel epigenetic program. As the results of gene enrichment, pathways analysis showed that TAPBP, BDNF, and SORBS2 linked together by inflammatory pathways. Hypermethylation in these genes might play a crucial rule in the co-occurrence of obesity and mood disorders.

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“…hPSCs (NIH Codes: WA01, H1) were maintained on irradiated CF1 feeder cells as described in our previous paper ( 20 ). Before lentiviral transduction and hematopoietic differentiation, hPSCs were maintained in chemically defined mTeSR™1 medium (StemCell Technologies, Canada) without feeder as described previously ( 21 ). The 293T cells were maintained in DMEM medium (Invitrogen, USA) supplemented with 10% fetal bovine serum (FBS, Hyclone, USA).…”

Section: Methodsmentioning

confidence: 99%

Single-cell qPCR facilitates the optimization of hematopoietic differentiation in hPSCs/OP9 coculture system

Chen

Jiang

Xiao

et al. 2018

Braz J Med Biol Res

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Human pluripotent stem cells (hPSCs)/OP9 coculture system is a widely used hematopoietic differentiation approach. The limited understanding of this process leads to its low efficiency. Thus, we used single-cell qPCR to reveal the gene expression profiles of individual CD34+ cells from different stages of differentiation. According to the dynamic gene expression of hematopoietic transcription factors, we overexpressed specific hematopoietic transcription factors (Gata2, Lmo2, Etv2, ERG, and SCL) at an early stage of hematopoietic differentiation. After overexpression, we generated more CD34+ cells with normal expression level of CD43 and CD31, which are used to define various hematopoietic progenitors. Furthermore, these CD34+ cells possessed normal differentiation potency in colony-forming unit assays and normal gene expression profiles. In this study, we demonstrated that single-cell qPCR can provide guidance for optimization of hematopoietic differentiation and transient overexpression of selected hematopoietic transcription factors can enhance hematopoietic differentiation.

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Generating hESCs with reduced immunogenicity by disrupting TAP1 or TAPBP

Cited by 20 publications

References 45 publications

Could the Heat Shock Proteins 70 Family Members Exacerbate the Immune Response in Multiple Sclerosis? An in Silico Study

Could the Heat Shock Proteins 70 Family Members Exacerbate the Immune Response in Multiple Sclerosis? An in Silico Study

The Epigenetic Overlap between Obesity and Mood Disorders: A Systematic Review

Single-cell qPCR facilitates the optimization of hematopoietic differentiation in hPSCs/OP9 coculture system

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