Generating human artery and vein cells from pluripotent stem cells highlights the arterial tropism of Nipah and Hendra viruses

Ang, Lay Teng; Nguyen, Alana T; Liu, Kevin J.; Chen, Angela; Xiong, Xunhao; Curtis, Matthew B.; Martin, Renata; Raftry, Brian C; Ng, Chun Yi; Vogel, Uwe; Lander, Angelika; Lesch, Benjamin J.; Fowler, Jonas L.; Holman, Alyssa R; Chai, Timothy; Vijayakumar, Siva; Suchy, Fabian P.; Nishimura, Toshinobu; Bhadury, Joydeep; Porteus, Matthew H.; Nakauchi, Hiromitsu; Cheung, Christine; George, Steven C.; Red‐Horse, Kristy; Prescott, Joseph; Loh, Kyle M.

doi:10.1016/j.cell.2022.05.024

Cited by 33 publications

(39 citation statements)

References 121 publications

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“…The stemness and differentiation capacity of the human induced pluripotent stem cells (hiPSCs) used in this study were previously validated 52,53 . All hiPSCs were tested for and maintained mycoplasma free.…”

Section: Hipsc Maintenancementioning

confidence: 99%

Spatially controlled construction of assembloids using bioprinting

Roth

Brunel

Huang

et al. 2023

Preprint

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The biofabrication of three-dimensional (3D) tissues that recapitulate organ-specific architecture and function would benefit from temporal and spatial control of cell-cell interactions. Bioprinting, while potentially capable of achieving such control, is poorly suited to spheroids and organoids with conserved cytoarchitectures that are susceptible to plastic deformation. Here, we develop a platform, termed Spheroid Transfer Assisted by Magnetic Printing (STAMP), consisting of an iron-oxide nanoparticle laden hydrogel and magnetized 3D printer to enable the controlled lifting, transport, and deposition of spheroids and organoids. We identify cellulose nanofibers as both an ideal biomaterial for encasing organoids with magnetic nanoparticles and a shear-thinning, self-healing support hydrogel for maintaining the spatial positioning of organoids to facilitate the generation of assembloids. We leverage STAMP to create precisely arranged assembloids composed of human pluripotent stem cell derived neural organoids and patient-derived glioma organoids. In doing so, we demonstrate the potential for the STAMP platform to construct assembloids which recapitulate key developmental processes and disease etiologies.

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Section: Hipsc Maintenancementioning

confidence: 99%

Spatially controlled construction of assembloids using bioprinting

Roth

Brunel

Huang

et al. 2023

Preprint

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“…A lack of physiologically accurate perfusable vasculature is one of the most important challenges limiting the expansion of multicellular ‘mini-organs’ known as organoids 151 . Advances in differentiation protocols and culture techniques for human pluripotent stem cells (hPSCs) 152 have resulted in the development of 3D self-organizing human blood vessel organoids, which are capable of forming complex vascular networks that recapitulate many of the mural cell–endothelial cell interactions known to be crucial for supporting structures such as the BBB 153 . These organoids, as well as several hPSC-derived liver 154 , 155 , kidney 156 and brain 157 organoids, have shown promising, though often partial, levels of host vascularization in vivo after transplantation into immunodeficient mice.…”

Section: Scrna-seq and Multimodal Omicsmentioning

confidence: 99%

Vascular endothelial cell development and diversity

2022

Self Cite

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Vascular endothelial cells form the inner layer of blood vessels where they have a key role in the development and maintenance of the functional circulatory system and provide paracrine support to surrounding non-vascular cells. Technical advances in the past 5 years in single-cell genomics and in in vivo genetic labelling have facilitated greater insights into endothelial cell development, plasticity and heterogeneity. These advances have also contributed to a new understanding of the timing of endothelial cell subtype differentiation and its relationship to the cell cycle. Identification of novel tissue-specific gene expression patterns in endothelial cells has led to the discovery of crucial signalling pathways and new interactions with other cell types that have key roles in both tissue maintenance and disease pathology. In this Review, we describe the latest findings in vascular endothelial cell development and diversity, which are often supported by large-scale, single-cell studies, and discuss the implications of these findings for vascular medicine. In addition, we highlight how techniques such as single-cell multimodal omics, which have become increasingly sophisticated over the past 2 years, are being utilized to study normal vascular physiology as well as functional perturbations in disease.

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“…A recent study reported a protocol for generation of human artery- and vein-specific ECs and illustrated the conflicting specification pathways. 62 However, it also highlighted the importance of the 2 identities in disease as two viruses preferentially targeted arterial ECs. In our organoids, the identity switch could be related to the observed upregulation of SULF1 (sulfatase 1) in mural cells.…”

Section: Bvos To Study Development and Diseasementioning

confidence: 99%

“…61 A recent protocol described the derivation of human artery- and vein-specific ECs from pluripotent stem cells. 62 It would be interesting to test whether these venous ECs have the ability to yield lymphatic ECs and if this approach could be combined with a 3-dimensional differentiation protocol.…”

Section: Organotypic Vasculature and Specific Functionsmentioning

confidence: 99%

Blood Vessel Organoids for Development and Disease

Salewskij

Penninger

2023

Circulation Research

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Despite enormous advances, cardiovascular disorders are still a major threat to global health and are responsible for one-third of deaths worldwide. Research for new therapeutics and the investigation of their effects on vascular parameters is often limited by species-specific pathways and a lack of high-throughput methods. The complex 3-dimensional environment of blood vessels, intricate cellular crosstalks, and organ-specific architectures further complicate the quest for a faithful human in vitro model. The development of novel organoid models of various tissues such as brain, gut, and kidney signified a leap for the field of personalized medicine and disease research. By utilizing either embryonic- or patient-derived stem cells, different developmental and pathological mechanisms can be modeled and investigated in a controlled in vitro environment. We have recently developed self-organizing human capillary blood vessel organoids that recapitulate key processes of vasculogenesis, angiogenesis, and diabetic vasculopathy. Since then, this organoid system has been utilized as a model for other disease processes, refined, and adapted for organ specificity. In this review, we will discuss novel and alternative approaches to blood vessel engineering and explore the cellular identity of engineered blood vessels in comparison to in vivo vasculature. Future perspectives and the therapeutic potential of blood vessel organoids will be discussed.

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Generating human artery and vein cells from pluripotent stem cells highlights the arterial tropism of Nipah and Hendra viruses

Cited by 33 publications

References 121 publications

Spatially controlled construction of assembloids using bioprinting

Spatially controlled construction of assembloids using bioprinting

Vascular endothelial cell development and diversity

Blood Vessel Organoids for Development and Disease

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