Generating mutant Aedes aegypti mosquitoes using the CRISPR/Cas9 system

Li, Hsing-Han; Li, Jian-Chiuan; Su, Matthew P.; Liu, Kun-Lin; Chen, Chun Hong

doi:10.1016/j.xpro.2021.100432

Cited by 6 publications

(1 citation statement)

References 11 publications

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“…A total of 11.66% of the Aaedsx female isoform-knockout larvae died, whereas only 3.33% of the AaeSxl-knockout eggs survived. These results indicate that CRISPR-Cas9 has the potential to reduce the population of dengue-causing vectors, which leads to a decrease in the level of infection in the environment [82]. Although CRISPR-Cas9 genome editing technology has high potential for application in various fields, especially vector control, to date, CRISPR-Cas9 technology has not been fully recognized and used as a companion to traditional vector control in Indonesia.…”

Section: Crispr-cas9-based Interventions Have the Potential To Modify...mentioning

confidence: 99%

CRISPR-Cas9 Genome Editing Technology for Zoonotic Disease Control in Indonesia: A Comprehensive Review

Adnyana,

Eljatin,

Sudaryati

et al. 2024

JMHT

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The increasing emergence of zoonotic diseases originating from vectors, rodents, mammals, and others has increased the potential for outbreaks of pandemics in the Indonesian territory. Although control and prevention have been implemented, these efforts have not yet revealed a bright spot; therefore, elaboration with advanced CRISPR-Cas9 technology is a way to accelerate efforts to control zoonotic diseases in Indonesia. However, there is limited literature on this topic. This review aims to comprehensively describe, identify, and summarize the application of CRISPR-Cas9 genome-editing technology in zoonotic disease control in Indonesia. Our findings show that CRISPR-Cas9 genome editing technology offers an innovative approach for zoonotic disease control by targeting disease vectors, modifying animal reservoirs, improving disease surveillance, enhancing vaccine development, and exploring traditional medicine candidates and immunotherapy. The high level of precision, efficiency, and versatility in targeting genomes capable of disrupting, damaging, and disrupting the disease transmission cycle in pathogens makes CRISPR-Cas9 highly effective. However, challenges such as off-target impacts, regulatory complexity, and ethical considerations must be overcome with inter- and multidisciplinary collaboration to promote transparency, equity, and public engagement throughout the process of implementing this technology in the field, especially in Indonesia.

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Section: Crispr-cas9-based Interventions Have the Potential To Modify...mentioning

confidence: 99%

CRISPR-Cas9 Genome Editing Technology for Zoonotic Disease Control in Indonesia: A Comprehensive Review

Adnyana,

Eljatin,

Sudaryati

et al. 2024

JMHT

View full text Add to dashboard Cite

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CRISPR-Mediated Genome Engineering in Aedes aegypti

Sun

McMeniman

et al. 2022

Methods in Molecular Biology

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miR-277 targets the proapoptotic gene-hid to ameliorate Aβ42-mediated neurodegeneration in Alzheimer’s model

Deshpande,

Chen,

Chimata

et al. 2024

Cell Death Dis

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Alzheimer’s disease (AD), an age-related progressive neurodegenerative disorder, exhibits reduced cognitive function with no cure to date. One of the reasons for AD is the accumulation of Amyloid-beta 42 (Aβ42) plaque(s) that trigger aberrant gene expression and signaling, which results in neuronal cell death by an unknown mechanism(s). Misexpression of human Aβ42 in the developing retina of Drosophila exhibits AD-like neuropathology. Small non-coding RNAs, microRNAs (miRNAs), post-transcriptionally regulate the expression of their target genes and thereby regulate different signaling pathways. In a forward genetic screen, we identified miR-277 (human ortholog is hsa-miR-3660) as a genetic modifier of Aβ42-mediated neurodegeneration. Loss-of-function of miR-277 enhances the Aβ42-mediated neurodegeneration. Whereas gain-of-function of miR-277 in the GMR > Aβ42 background downregulates cell death to maintain the number of neurons and thereby restores the retinal axonal targeting defects indicating the functional rescue. In addition, gain-of-function of miR-277 rescues the eclosion- and climbing assays defects observed in GMR > Aβ42 background. Thus, gain-of-function of miR-277 rescues both structurally as well as functionally the Aβ42-mediated neurodegeneration. Furthermore, we identified head involution defective (hid), an evolutionarily conserved proapoptotic gene, as one of the targets of miR-277 and validated these results using luciferase- and qPCR -assays. In the GMR > Aβ42 background, the gain-of-function of miR-277 results in the reduction of hid transcript levels to one-third of its levels as compared to GMR > Aβ42 background alone. Here, we provide a novel molecular mechanism where miR-277 targets and downregulates proapoptotic gene, hid, transcript levels to rescue Aβ42-mediated neurodegeneration by blocking cell death. These studies shed light on molecular mechanism(s) that mediate cell death response following Aβ42 accumulation seen in neurodegenerative disorders in humans and provide new therapeutic targets for neurodegeneration.

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Generating mutant Aedes aegypti mosquitoes using the CRISPR/Cas9 system

Cited by 6 publications

References 11 publications

CRISPR-Cas9 Genome Editing Technology for Zoonotic Disease Control in Indonesia: A Comprehensive Review

CRISPR-Cas9 Genome Editing Technology for Zoonotic Disease Control in Indonesia: A Comprehensive Review

CRISPR-Mediated Genome Engineering in Aedes aegypti

miR-277 targets the proapoptotic gene-hid to ameliorate Aβ42-mediated neurodegeneration in Alzheimer’s model

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