Generation and characterization of new monoclonal antibodies against swine origin 2009 influenza A (H1N1) virus and evaluation of their prophylactic and therapeutic efficacy in a mouse model

Wang, Sheng-Fan; Tseng, Sung-Pin; Loh, El Wui; Wang, Wen Hung; Li, Ming Chun; Chen, Kuan Hsuan; Tsai, Wen Chun; Lee, Yuan Ming; Chen, Huan Yuan; Liu, Fu Tong; Chen, Yi Ming Arthur; Huang, Jason C.

doi:10.1016/j.dci.2016.10.010

Cited by 7 publications

(9 citation statements)

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“…Because of its existing postmodification and correct folding ability in the newly generated proteins, the baculovirus-insect expression system has been proven to be a better method to produce functional or therapeutic antibodies against influenza virus infection . In addition, the recombinant trimeric HAs, which displayed similar structure with the native form of HA on the influenza envelope, could induce higher titers of type-specific antibodies. , Here, we used a similar strategy to generate H7-mAb for H7N9 virus detection.…”

Section: Resultsmentioning

confidence: 99%

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Simple Strategy for Rapid and Sensitive Detection of Avian Influenza A H7N9 Virus Based on Intensity-Modulated SPR Biosensor and New Generated Antibody

et al. 2018

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In 2013 a new reassortant avian influenza A H7N9 virus emerged in China, causing human infection with high mortality. An accurate and timely diagnosis is crucial for controlling the outbreaks of the disease. We therefore propose a simple strategy for rapidly and sensitively detecting the H7N9 virus using an intensity-modulated surface plasmon resonance (IM-SPR) biosensor integrated with a new generated monoclonal antibody. The novel antibody exhibits significant specificity to recognize H7N9 virus compared with other clinical human influenza isolates (p < 0.01). Experimentally, the detection limit of the proposed approach for H7N9 virus detection is estimated to be 144 copies/mL, which is a 20-fold increase in sensitivity compared with homemade target-captured ELISA using the identical antibody. For the measurement of mimic clinical specimens containing the H7N9 virus mixed with nasal mucosa from flu-like syndrome patients, the detection limit is calculated to be 402 copies/mL, which is better than conventional influenza detection assays; quantitative reverse transcription polymerase chain reaction (qRT-PCR) and rapid influenza diagnostic test (RIDT). Most importantly, the assay time took less than 10 min. Combined, the results of this study indicate that the proposed simple strategy demonstrates high sensitivity and time-saving in H7N9 virus detection. By incorporating a high specific recognizer, the proposed technique has the potential to be used in applications and development of other emerging or re-emerging microbe detection platforms.

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Section: Resultsmentioning

confidence: 99%

“…F5506). After a final boost, the mice splenocytes were collected and subjected to fusion with NS-1 cells (myeloma cells), as described previously. , After serial dilution, several clones that produced specific individual antibodies were selected. , …”

Section: Methodsmentioning

confidence: 99%

Simple Strategy for Rapid and Sensitive Detection of Avian Influenza A H7N9 Virus Based on Intensity-Modulated SPR Biosensor and New Generated Antibody

et al. 2018

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“…Influenza A viruses are subtyped according to the envelope glycoproteins, hemagglutinin (HA) and NA, expressed on the viral surface. HA binding to cell receptors has been shown to play an important role in both viral invasion and membrane fusion, and represents one of the main targets of neutralizing antibodies 16 …”

Section: Introductionmentioning

confidence: 99%

“…HA binding to cell receptors has been shown to play an important role in both viral invasion and membrane fusion, and represents one of the main targets of neutralizing antibodies. 16 In this study, HA-specific mAbs were produced by immunizing mice with an H1N1 virus. The obtained mAbs were characterized and functionally evaluated for the ability to neutralize H1N1.…”

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Generation, characterization, and protective ability of mouse monoclonal antibodies against the HA of A (H1N1) influenza virus

Wang

Yang

Xiao

et al. 2022

Journal of Medical Virology

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Influenza virus infections pose a continuous threat to human health. Although vaccines function as a preventive and protective tool, they may not be effective due to antigen drift or an inaccurate prediction of epidemic strains. Monoclonal antibodies (mAbs) have attracted wide attention as a promising therapeutic method for influenza virus infections. In this study, three hemagglutinin (HA)-specific mAbs, named 2A1, 2H4, and 2G2, respectively, were derived from mice immunized with the HA protein from A/Michigan/45/2015(H1N1). The isolated mAbs all displayed hemagglutination inhibition activity and the 2G2 mAb exhibited the strongest neutralization effect. Two amino acid mutations (A198E and G173E), recognized in the process of selection of mAb-resistant mutants, were located in antigenic site Sb and Ca1, respectively. In prophylactic experiments, all three mAbs could achieve 100% protection in mice infected with a lethal dose of A/Michigan/45/2015(H1N1).A dose of 1 mg/kg for 2H4 and 2G2 was sufficient to achieve a full protective effect.Therapeutic experiments showed that all three mAbs could protect mice from death if they received the mAb administration at 6 h postinfection, and 2G2 was still protective after 24 h. Our findings indicate that these three mAbs may have potential prevention and treatment value in an H1N1 epidemic, as well as in the study of antigen epitope recognition.

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“…Reports indicated that an estimated 75% of all known EIDs originate from some type of animal reservoir [ 31 , 32 ]. Some examples of zoonotic disease include HIV/AIDS, SARS, MERS, several hemorrhagic fevers, Lyme disease, plague, and avian or swine origin influenza [ 32 , 33 , 34 , 35 , 36 , 37 ]. Each of these diseases possesses a unique etiology and a direct or indirect transmission route.…”

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confidence: 99%

Emerging and Re-Emerging Diseases

et al. 2021

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Generation and characterization of new monoclonal antibodies against swine origin 2009 influenza A (H1N1) virus and evaluation of their prophylactic and therapeutic efficacy in a mouse model

Cited by 7 publications

References 30 publications

Simple Strategy for Rapid and Sensitive Detection of Avian Influenza A H7N9 Virus Based on Intensity-Modulated SPR Biosensor and New Generated Antibody

Simple Strategy for Rapid and Sensitive Detection of Avian Influenza A H7N9 Virus Based on Intensity-Modulated SPR Biosensor and New Generated Antibody

Generation, characterization, and protective ability of mouse monoclonal antibodies against the HA of A (H1N1) influenza virus

Emerging and Re-Emerging Diseases

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