Generation and ethylene production of transgenic carnations harboring ACC synthase cDNA in sense or antisense orientation

Iwazaki, Yujiro; Kosugi, Yusuke; Waki, Keisuke; Yoshioka, T.; Satoh, Shigeru

doi:10.37855/jah.2004.v06i02.15

Cited by 11 publications

(1 citation statement)

References 17 publications

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“…After two cycles of crossing and selection, the rate of browning senescence patterns (browning plus wilting with browning) increased to 19.3%. In carnation, the relationship between senescence patterns and vase life has been well characterized: petal inrolling at the onset of wilting is a well-known characteristic of ethylene-dependent senescence of normal carnation flowers (Iwazaki et al, 2004;Otsu et al, 2007;Satoh, 2011). In contrast, desiccation and browning of petals are characteristics of ethylene-independent senescence in carnation cultivars with low ethylene production.…”

Section: Discussionmentioning

confidence: 99%

Breeding for Long Vase Life in Dahlia (<i>Dahlia variabilis</i>) Cut Flowers

Onozaki

Azuma

2019

Hort. J.

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Flower vase life is one of the most important traits for ornamental plants. The vase life of cut dahlia (Dahlia variabilis) flowers is very short, and genetic improvement of this trait is desirable. We started a breeding research program in 2014 to improve the vase life of dahlia flowers using conventional cross-breeding techniques. We found large significant differences in flower vase life among 24 dahlia cultivars: Nine cultivars had long vase life (e.g., 'Syukuhai', 'Rinka', and 'Micchan'); eight had normal vase life (e.g., 'Kamakura', 'Agitate', and 'Benifusya'); and seven had short vase life (e.g., 'Gin-Ei', 'Port Light Pair Beauty', and 'Yumesuiren'). We used 22 cultivars as initial breeding materials, repeatedly crossed them, and selected promising offspring with long vase life for three generations from 2014 to 2018. Two cycles of selection and crossing led to a 1.7-day increase in vase life (population mean) from the first to the third generation, clearly showing that this approach can extend the vase life of dahlia flowers. The mean vase life of 'Kamakura', a leading white dahlia cultivar in Japan, was 5.0-6.2 days in distilled water, 6.0-6.8 days in an isothiazolinic antibacterial agent CMIT/MIT solution (5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3one) and 6.0-7.6 days in a GLA solution (10 g•L −1 glucose, 0.5 ml•L −1 CMIT/MIT, and 50 mg•L −1 aluminum sulfate), whereas in six finally selected lines it was 6.2-12.0 days in distilled water, 6.6-10.2 days in CMIT/MIT solution, and 9.4-13.6 days in GLA solution (1.4-2.1 times that in 'Kamakura'). In particular, the selected second-generation line 606-46 showed a stably longer vase life than 'Kamakura'. 'Micchan', which has a long vase life, was a common progenitor used for breeding of parental lines in cross combinations with long vase life in the second generation and all cross combinations in the third generation. The final six selected lines with long vase life were all progeny of 'Micchan'. Our results strongly suggest that 'Micchan' has genes related to long flower vase life, and that the trait is heritable.

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Section: Discussionmentioning

confidence: 99%

Breeding for Long Vase Life in Dahlia (<i>Dahlia variabilis</i>) Cut Flowers

Onozaki

Azuma

2019

Hort. J.

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Breeding and Genetics for Shelf and Vase Life

Mibus

2018

Handbook of Plant Breeding

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Analysis of genomic DNA of DcACS1, a 1-aminocyclopropane-1-carboxylate synthase gene, expressed in senescing petals of carnation (Dianthus caryophyllus) and its orthologous genes in D. superbus var. longicalycinus

et al. 2010

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Carnation (Dianthus caryophyllus) flowers exhibit climacteric ethylene production followed by petal wilting, a senescence symptom. DcACS1, which encodes 1-aminocyclopropane-1-carboxylate synthase (ACS), is a gene involved in this phenomenon. We determined the genomic DNA structure of DcACS1 by genomic PCR. In the genome of 'Light Pink Barbara', we found two distinct nucleotide sequences: one corresponding to the gene previously shown as DcACS1, designated here as DcACS1a, and the other novel one designated as DcACS1b. It was revealed that both DcACS1a and DcACS1b have five exons and four introns. These two genes had almost identical nucleotide sequences in exons, but not in some introns and 3'-UTR. Analysis of transcript accumulation revealed that DcACS1b is expressed in senescing petals as well as DcACS1a. Genomic PCR analysis of 32 carnation cultivars showed that most cultivars have only DcACS1a and some have both DcACS1a and DcACS1b. Moreover, we found two DcACS1 orthologous genes with different nucleotide sequences from D. superbus var. longicalycinus, and designated them as DsuACS1a and DsuACS1b. Petals of D. superbus var. longicalycinus produced ethylene in response to exogenous ethylene, accompanying accumulation of DsuACS1 transcripts. These data suggest that climacteric ethylene production in flowers was genetically established before the cultivation of carnation.

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Generation and ethylene production of transgenic carnations harboring ACC synthase cDNA in sense or antisense orientation

Cited by 11 publications

References 17 publications

Breeding for Long Vase Life in Dahlia (<i>Dahlia variabilis</i>) Cut Flowers

Breeding for Long Vase Life in Dahlia (<i>Dahlia variabilis</i>) Cut Flowers

Breeding and Genetics for Shelf and Vase Life

Analysis of genomic DNA of DcACS1, a 1-aminocyclopropane-1-carboxylate synthase gene, expressed in senescing petals of carnation (Dianthus caryophyllus) and its orthologous genes in D. superbus var. longicalycinus

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