Generation and ex vivo expansion of HTLV‐1 specific CD8 cytotoxic T‐lymphocytes for adoptive immunotherapy

Peshwa, Madhusudan V.; Page, Laura A.; Qian, Lichuan; Yang, Demao; Schooten, W. C. A. Van

doi:10.1002/(sici)1097-0290(19960605)50:5<529::aid-bit7>3.3.co;2-p

Cited by 8 publications

(3 citation statements)

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“…Cells used for somatic therapies include hematopoietic cells used for bone marrow or peripheral blood progenitor cell transplantation (Collins et al, 1996;Koller et al, 1993;McAdams et al, 1996), and immune cells such as natural killer (NK) cells (Miller et al, 1994;Pierson et al, 1996), lymphokine-activated killer (LAK) cells (Melder et al, 1989;Muul et al, 1987), tumor-infiltrating lymphocytes (TIL) (Rosenberg et al, 1993;Whiteside and Parmiani, 1994), and antigen-specific cytotoxic T lymphocytes (CTL) (Peshwa et al, 1996;Riddell et al, 1992a,b;Rivoltini et al, 1995;Salgaller et al, 1994;Stevens et al, 1995). These therapies involve the transfer of large numbers of autologous cultured cells to patients.…”

Section: Introductionmentioning

confidence: 99%

Increased agitation intensity increases CD13 receptor surface content and mRNA levels, and alters the metabolism of HL60 cells cultured in stirred tank bioreactors

McDowell¹,

Papoutsakis²

1998

Biotechnol. Bioeng.

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Flow cytometry and Northern blotting were used to examine the effects of hydrodynamic forces in stirred tank bioreactors on CD13 receptor surface content and mRNA levels of HL60 (human promyelocytic leukemia) cells. A step increase in agitation rate from 80 to 300 or 400 rpm reduced the apparent HL60 growth rate in a dose‐dependent manner. This step increase in agitation rate (to 300 or 400 rpm) also increased the CD13 receptor surface content on averge by 30% and 100%, respectively. This increase in CD13 receptor surface content was correlated with a 10% and a 60% increase in CD13 mRNA levels. We also observed a significant and very reproducible drop in CD13 expression over the course of a batch bioreactor run (80 rpm). Although we have no explanation for this, we show that the decrease in CD13 receptor surface content can be (at least partially, if not fully) explained by the corresponding decrease in CD13 mRNA. HL60 cell cultures agitated at 300 and 400 rpm exhibited glucose consumption and lactate production rates that were approximately 40% and 90% greater than values of the cultures agitated at 80 rpm. The physiological and practical implications of these results are discussed. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 60: 239–250, 1998.

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Section: Introductionmentioning

confidence: 99%

Increased agitation intensity increases CD13 receptor surface content and mRNA levels, and alters the metabolism of HL60 cells cultured in stirred tank bioreactors

McDowell¹,

Papoutsakis²

1998

Biotechnol. Bioeng.

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“…Taking advantage of exogenous supplementation of growth and differentiation factors, there has been already limited success in the regeneration of mammalian bone, skin, blood vessels, and spinal cord (277). Furthermore, such factors are also used in cell therapies, a technology that includes the ex vivo manipulation of host or donor cells and is most often used for immunomodulatory therapy or hematopoietic cell reconstitution (278, 279). Immune and hematopoietic cell therapies employ suspended cell populations that are expanded ex vivo and reinfused into the blood stream.…”

Section: Impact Of Cell Cycle and Apoptosis On Metabolic Engineerimentioning

confidence: 99%

“…However, more recent approaches have sought to target more precisely specific tumor or viral antigens presented by cell surface MHC molecules. The goal of many of these therapies is the infusion of large numbers of antigen‐specific cytotoxic T lymphocytes which can be triggered via TcR engagement to lyse transformed or virally infected target cells (see above; 278). Similar cell therapies are also applied to hematopoietic stem cells (279).…”

Section: Impact Of Cell Cycle and Apoptosis On Metabolic Engineerimentioning

confidence: 99%

Molecular Regulation of Cell-Cycle Progression and Apoptosis in Mammalian Cells: Implications for Biotechnology

1998

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Regulation of the cell cycle and of programmed cell death (apoptosis) is essential for mammalian development and homeostasis. Furthermore, this regulation is fundamental to successful cell culture technology and tissue engineering. Therefore the molecular networks which regulate these processes are critical targets for drug development, gene therapy, and metabolic engineering. This review summarizes the genes, proteins, and interactions presently known to control apoptosis and cell-cycle progression. Knowledge of the networks summarized here and access to the component genes and proteins have already been applied successfully to guide research and development in bioprocess technology and medical treatment.

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Induction of prostate tumor-specific CD8+ cytotoxic T-lymphocytes in vitro using antigen-presenting cells pulsed with prostatic acid phosphatase peptide

et al. 1998

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Generation and ex vivo expansion of HTLV‐1 specific CD8 cytotoxic T‐lymphocytes for adoptive immunotherapy

Cited by 8 publications

References 1 publication

Increased agitation intensity increases CD13 receptor surface content and mRNA levels, and alters the metabolism of HL60 cells cultured in stirred tank bioreactors

Increased agitation intensity increases CD13 receptor surface content and mRNA levels, and alters the metabolism of HL60 cells cultured in stirred tank bioreactors

Molecular Regulation of Cell-Cycle Progression and Apoptosis in Mammalian Cells: Implications for Biotechnology

Induction of prostate tumor-specific CD8+ cytotoxic T-lymphocytes in vitro using antigen-presenting cells pulsed with prostatic acid phosphatase peptide

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