Generation and identification of anti‐inflammatory peptides from bovine <i>β</i>‐casein using enzyme preparations from cod and hog

Altmann, Karina; Wutkowski, Adam; Klempt, Martin; Clawin‐Rädecker, Ingrid; Meisel, Hans; Lorenzen, Peter Christian

doi:10.1002/jsfa.7159

Cited by 22 publications

(11 citation statements)

References 25 publications

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“…It was suggested that a group of large hydrophobic peptides were responsible for the antiinflammatory activity. The hydrolysis of β-casein using cod trypsin has also been reported to have anti-inflammatory activity in kidney cells and larger peptides (> 5 kDa) were reported to have higher activity compared with lower molecular mass peptides (Altmann et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…The antiinflammatory activity of all seven hydrolysate 5 kDa permeates may be linked to NF-κB activation as the protein expression of p65 was inhibited in all cases. Milk-derived hydrolysates with anti-inflammatory activity have previously been reported to act through the NF-κB pathway in different cell lines (Altmann et al, 2016;Malinowski et al, 2014;Marcone, Haughton, Simpson, Belton, & FitzGerald, 2015;Nielsen, Theil, Larson, & Parup, 2012). To the best of our knowledge, our study is the first to examine the molecular mechanism behind the anti-inflammatory activity of bovine casein hydrolysates in macrophages.…”

Section: Discussionmentioning

confidence: 99%

“…The tryptic hydrolysis of casein generated a hydrolysate with immune enhancing effects in mouse macrophages (Kazlauskaite et al, 2005), while β-casein and several peptides within β-casein have been reported to decrease proliferation in murine spleen cells (Bonomi et al, 2011). Studies have also reported that the activity of NF-κβ, one of the major inflammatory signalling pathways, may be reduced in cells exposed to casein hydrolysates (Altmann et al, 2016;Malinowski, Klempt, Clawin-Rädecker, Lorenzen, & Meisel, 2014). The hydrolysis of casein using mammalian, bacterial or plant derived enzymes has also been reported to have immunomodulating effects on Jurkat T cells in other studies (Cermeño et al, 2016;Lahart et al, 2011;Phelan et al, 2009).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

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Immunomodulatory activity of 5 kDa permeate fractions of casein hydrolysates generated using a range of enzymes in Jurkat T cells and RAW264.7 macrophages

O'Sullivan

O’Callaghan

O’Keeffe

et al. 2019

International Dairy Journal

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Accepted Manuscriptmentioning

confidence: 99%

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Immunomodulatory activity of 5 kDa permeate fractions of casein hydrolysates generated using a range of enzymes in Jurkat T cells and RAW264.7 macrophages

O'Sullivan

O’Callaghan

O’Keeffe

et al. 2019

International Dairy Journal

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“…These peptides may affect the cardiovascular (casokinins, casoplatelins), nervous (antagonists and agonists of opiate receptors), immune (immunomodulators) and digestive (regulators of motility and secretion in the gastroenteric tract, metal-binding peptides) systems. New kinds of biological action of casein peptides are discovered [4,5]. Casein belongs to heterogeneous proteins and consists of four basic (α S1 -CN, α S2 -CN, β-CN and κ-CN) and numerous minor fractions.…”

mentioning

confidence: 99%

Isolation of κ-CN-1P and Β-CN-5Р fractions from native casein micelles

Yukalo¹

2018

Ukr.Biochem.J.

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Proteins of the casein complex of milk arouse considerable interest as the precursors of biologically active peptides which are capable of influencing various physiological systems of the body (digestive, nervous, cardiovascular, and immune). It has been established that various bioactive peptides are unevenly located in the structure of casein fractions. In this connection, there appeared a need to separate individual fractions of this protein for studying the pathways of formation and properties of bioactive casein peptides. to minimize negative effects of the purification procedure, we used the gel filtration on Sefarose 2B to produce native casein micelles and repeating filtration on Sephadex G-150 to separate individual fractions. As a result, according to electrophoretic analysis, casein micelles with a characteristic protein composition were obtained. taking into account the similarity of the molecular weight of components for their dividing the repeated gel filtration was carried out with separating the chromatograms into sectors. The composition of the combined fractions of each sector was analyzed by electrophoresis. this approach allowed isolating two electrophoretically homogeneous proteins from native casein micelles-κ-CN-1P and β-CN-5P, as well as to obtain a substantially purified (> 83%) α S1-CN-XP. Isolated caseins without the influence of extreme factors can be used to study natural bioactive peptides. K e y w o r d s: native casein micelles, casein fractions, gel filtration.

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“…However, capability of such hydrolysates to target ENS maturation has never been investigated. Therefore, we analyzed the potential of a recently characterized bovine tryptic β-CN hydrolysate presenting anti-inflammatory properties (Altmann et al, 2016) to nutritionally target ENS development.…”

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confidence: 99%

Short communication: Tryptic β-casein hydrolysate modulates enteric nervous system development in primary culture

Cossais¹,

Clawin‐Rädecker

Lorenzen

et al. 2017

Journal of Dairy Science

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The intestinal tract of the newborn is particularly sensitive to gastrointestinal disorders, such as infantile diarrhea or necrotizing colitis. Perinatal development of the gut also encompasses the maturation of the enteric nervous system (ENS), a main regulator of intestinal motility and barrier functions. It was recently shown that ENS maturation can be enhanced by nutritional factors to improve intestinal maturation. Bioactivity of milk proteins is often latent, requiring the release of bioactive peptides from inactive native proteins. Several casein-derived hydrolysates presenting immunomodulatory properties have been described recently. Furthermore, accumulating data indicate that milk-derived hydrolysate can enhance gut maturation and enrichment of milk formula with such hydrolysates has recently been proposed. However, the capability of milk-derived bioactive hydrolysate to target ENS maturation has not been analyzed so far. We, therefore, investigated the potential of a recently described tryptic β-casein hydrolysate to modulate ENS growth parameters in an in vitro model of rat primary culture of ENS. Rat primary cultures of ENS were incubated with a bioactive tryptic β-casein hydrolysate and compared with untreated controls or to cultures treated with native β-casein or a Prolyve β-casein hydrolysate (Lyven, Colombelles, France). Differentiation of enteric neurons and enteric glial cells, and establishment of enteric neural network were analyzed using immunohistochemistry and quantitative PCR. Effect of tryptic β-casein hydrolysate on bone morphogenetic proteins (BMP)/Smad pathway, an essential regulator of ENS development, was further assessed using quantitative PCR and immunochemistry. Tryptic β-casein hydrolysate stimulated neurite outgrowth and simultaneously modulated the formation of enteric ganglia-like structures, whereas native β-casein or Prolyve β-casein hydrolysate did not. Additionally, treatment with tryptic bioactive β-casein hydrolysate increased the expression of the glial marker glial fibrillary acidic protein and induced profound modifications of enteric glial cells morphology. Finally, expression of BMP2 and BMP4 and activation of Smad1/5 was altered after treatment with tryptic bioactive β-casein hydrolysate. Our data suggests that this milk-derived bioactive hydrolysate modulates ENS maturation through the regulation of BMP/Smad-signaling pathway. This study supports the need for further investigation on the influence of milk-derived bioactive peptides on ENS and intestinal maturation in vivo.

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Generation and identification of anti‐inflammatory peptides from bovine β‐casein using enzyme preparations from cod and hog

Cited by 22 publications

References 25 publications

Immunomodulatory activity of 5 kDa permeate fractions of casein hydrolysates generated using a range of enzymes in Jurkat T cells and RAW264.7 macrophages

Immunomodulatory activity of 5 kDa permeate fractions of casein hydrolysates generated using a range of enzymes in Jurkat T cells and RAW264.7 macrophages

Isolation of κ-CN-1P and Β-CN-5Р fractions from native casein micelles

Short communication: Tryptic β-casein hydrolysate modulates enteric nervous system development in primary culture

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