Generation and manipulation of magnetic multicellular spheroids

Ho, Vincent H.B.; Müller, Karin H.; Barcza, Alexander; Chen, Rongjun; Slater, Nigel K.H.

doi:10.1016/j.biomaterials.2009.12.047

Cited by 82 publications

(88 citation statements)

References 37 publications

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“…2015, 8(8): 2515-2532 implementation of MNPs-modified cells in fabrication of artificial constructs imitating vascular tissue [4], skeletal muscle tissues [5], and keratinocyte sheets [6]. Magnetic functionalization of cells was also employed in fabrication of cellular spheroids [7], which are regarded as three-dimensional building blocks in tissue engineering and a prospective tool in pharmaceutical applications (i.e., drug screening) [8] and tumor studies [9]. MNPs-modified cells were also employed for fabrication of aortic valve mimics using a magnetic levitation procedure [10].…”

Section: Introductionmentioning

confidence: 99%

Cell surface engineering with polyelectrolyte-stabilized magnetic nanoparticles: A facile approach for fabrication of artificial multicellular tissue-mimicking clusters

et al. 2015

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Regenerative medicine requires new ways to assemble and manipulate cells for fabrication of tissue-like constructs. Here we report a novel approach for cell surface engineering of human cells using polymer-stabilized magnetic nanoparticles (MNPs). Cationic polyelectrolyte-coated MNPs are directly deposited onto cellular membranes, producing a mesoporous semi-permeable layer and rendering cells magnetically responsive. Deposition of MNPs can be completed within minutes, under cell-friendly conditions (room temperature and physiologic media). Microscopy (TEM, SEM, AFM, and enhanced dark-field imaging) revealed the intercalation of nanoparticles into the cellular microvilli network. A detailed viability investigation was performed and suggested that MNPs do not inhibit membrane integrity, enzymatic activity, adhesion, proliferation, or cytoskeleton formation, and do not induce apoptosis in either cancer or primary cells. Finally, magnetically functionalized cells were employed to fabricate viable layered planar (two-cell layers) cell sheets and 3D multicellular spheroids.

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Section: Introductionmentioning

confidence: 99%

Cell surface engineering with polyelectrolyte-stabilized magnetic nanoparticles: A facile approach for fabrication of artificial multicellular tissue-mimicking clusters

et al. 2015

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“…Finally, using magnetically functionalized electrospun matrices with magnetic nanoparticles [23] as well as using tissue spheroids biofabricated from cells labelled with magnetic nanoparticles [24][25][26][27] will enable the development of novel magnetic 3D bioprinting technology based on principles of magnetic levitation or translocation of tissue constructs using magnetic forces [28][29][30] .…”

Section: Discussionmentioning

confidence: 99%

“…It has been demonstrated that tissue spheroids can attach, spread and fuse on synthetic electrospun matrices [21,22] . Moreover, recently reported magnetic functionalization of electrospun synthetic matrices with magnetic nanoparticles [23] as well as biofabrication of tissue spheroids from cells labelled with magnetic nanoparticles [24][25][26][27] allow the development of magnetic forcesdriven biofabrication and even 3D magnetic bioprinting based on principles of magnetic levitation [28][29][30] . Thus, application of nanotechnology can enable development of novel technology of magnetic 3D bioprinting.…”

Section: Introductionmentioning

confidence: 99%

Patterning of tissue spheroids biofabricated from human fibroblasts on the surface of electrospun polyurethane matrix using 3D bioprinter

Mironov¹,

Khesuani²,

Буланова³

et al. 2024

IJB

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Patterning of tissue spheroids biofabricated from human fibroblasts on the surface of electrospun polyurethane matrix using 3D bioprinter. © 2016 Elizabeth V. Koudan, et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. 45 RESEARCH ARTICLEPatterning of tissue spheroids biofabricated from human fibroblasts on the surface of electrospun polyurethane matrix using 3D bioprinter Abstract: Organ printing is a computer-aided additive biofabrication of functional three-dimensional human tissue and organ constructs according to digital model using the tissue spheroids as building blocks. The fundamental biological principle of organ printing technology is a phenomenon of tissue fusion. Closely placed tissue spheroids undergo tissue fusion driven by surface tension forces. In order to ensure tissue fusion in the course of post-printing, tissue spheroids must be placed and maintained close to each other. We report here that tissue spheroids biofabricated from primary human fibroblasts could be placed and maintained on the surface of biocompatible electrospun polyurethane matrix using 3D bioprinter according to desirable pattern. The patterned tissue spheroids attach to polyurethane matrix during several hours and became completely spread during several days. Tissue constructions biofabricated by spreading of patterned tissue spheroids on the biocompatible electrospun polyurethane matrix is a novel technological platform for 3D bioprinting of human tissue and organs.

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“…For this application, gradients of the magnetic field were achieved using a pin holder and used for a 3D cell culture array. 33 Ho et al 95 initially formed random arrangement of multicellular spheroids of HeLa cells after labeling them with paramag-netic particles. These spheroids were patterned rapidly with magnetic fields.…”

Section: Magnetophoretic Trappingmentioning

confidence: 99%

Exploitation of physical and chemical constraints for three-dimensional microtissue construction in microfluidics

Choudhury

Iliescu

et al. 2011

Biomicrofluidics

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There are a plethora of approaches to construct microtissues as building blocks for the repair and regeneration of larger and complex tissues. Here we focus on various physical and chemical trapping methods for engineering three-dimensional microtissue constructs in microfluidic systems that recapitulate the in vivo tissue microstructures and functions. Advances in these in vitro tissue models have enabled various applications, including drug screening, disease or injury models, and cellbased biosensors. The future would see strides toward the mesoscale control of even finer tissue microstructures and the scaling of various designs for high throughput applications. These tools and knowledge will establish the foundation for precision engineering of complex tissues of the internal organs for biomedical applications.

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Generation and manipulation of magnetic multicellular spheroids

Cited by 82 publications

References 37 publications

Cell surface engineering with polyelectrolyte-stabilized magnetic nanoparticles: A facile approach for fabrication of artificial multicellular tissue-mimicking clusters

Cell surface engineering with polyelectrolyte-stabilized magnetic nanoparticles: A facile approach for fabrication of artificial multicellular tissue-mimicking clusters

Patterning of tissue spheroids biofabricated from human fibroblasts on the surface of electrospun polyurethane matrix using 3D bioprinter

Exploitation of physical and chemical constraints for three-dimensional microtissue construction in microfluidics

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