Generation, characterization and ELISA of monospecific antibodies against the subunits of a Ca2+-dependent protein kinase and a Ca2+-transport ATPase from rabbit skeletal muscle

Böhm, H.; Gehr, J. K. H. Petersen-Von; Neubauer, H.; Mehnert, F; Jennissen, H. P.

doi:10.1016/0022-1759(84)90185-6

Cited by 8 publications

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Laminin immunized monkeys develop sera toxic to cultured rat embryos and fail to reproduce

et al. 1989

View full text Add to dashboard Cite

In a previous study antilaminin antibodies in a monkey with a poor reproductive history were found to be the cause of serum toxicity to cultured rat embryos. In the present study four monkeys were immunized with murine tumor laminin and a fifth with bovine serum albumin. Subsequently, sera from only the laminin immunized monkeys became toxic to cultured rat embryos. This serum toxicity was not mediated by complement but did require the antibody to have a divalent structure. Finally, mating trials were conducted with two of the laminin immunized monkeys that previously had excellent reproductive histories. Based on progesterone levels and observation the monkeys continued to have normal menstrual cycles but failed to initiate a successful pregnancy following immunization in over 2 years of mating trials. These data demonstrated that antibodies against laminin could have prevented conception or could have interrupted pregnancy because of embryotoxicity or failure of implantation.

show abstract

Laminin immunized monkeys develop sera toxic to cultured rat embryos and fail to reproduce

et al. 1989

View full text Add to dashboard Cite

show abstract

Toxicity of sera from individuals with Chagas' disease to cultured rat embryos: Role of antibodies to laminin

1991

View full text Add to dashboard Cite

In previous studies antilaminin antibodies in the sera of immunized monkeys and rats were found to be toxic to cultured rat embryos. In order to extend these studies to humans, head-fold stage rat embryos were cultured for 48 hours on ten different serum samples from individuals with Chagas' disease. All embryos (n = 20) cultured on these sera were found to be abnormal. Using ELISA, Western immunoblot, and indirect immunofluorescence it could be shown that antibodies in these sera reacted with laminin. That these antilaminin antibodies were, at least in part, responsible for the toxicity was indicated 1) by reduced cultured embryo toxicity for six of seven serum samples after pre-absorption with purified laminin, 2) by demonstrating a relationship between the amount of affinity-purified antilaminin IgG added to control serum for culture and the severity of embryo abnormalities seen at the end of culture, and 3) by the sera's failing to react with other basement membrane proteins, including type IV collagen, fibronectin, osteonectin, and heparan sulfate proteoglycan.

show abstract

Ubiquitin and the enigma of intracellular protein degradation

Jennissen¹

1995

EJB Reviews

View full text Add to dashboard Cite

Generation, characterization and ELISA of monospecific antibodies against the subunits of a Ca2+-dependent protein kinase and a Ca2+-transport ATPase from rabbit skeletal muscle

Cited by 8 publications

References 21 publications

Laminin immunized monkeys develop sera toxic to cultured rat embryos and fail to reproduce

Laminin immunized monkeys develop sera toxic to cultured rat embryos and fail to reproduce

Toxicity of sera from individuals with Chagas' disease to cultured rat embryos: Role of antibodies to laminin

Ubiquitin and the enigma of intracellular protein degradation

Contact Info

Product

Resources

About