Generation of a flanking sequence‐tag database for activation‐tagging lines in japonica rice

Jeong, Dong‐Hoon; An, Shuqing; Park, Sunhee; Kang, Hong‐Gyu; Park, Gi-Gyeong; Kim, Sung-Ryul; Sim, Jayeon; Kim, Young-Ock; Kim, Minkyung; Kim, Seong-Ryong; Kim, Joowon; Shin, Moonsoo; Jung, Mooyoung; An, Gynheung

doi:10.1111/j.1365-313x.2005.02610.x

Cited by 315 publications

(311 citation statements)

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“…To examine the in planta function of OsSUT2, we isolated a null mutant of this gene from the rice T-DNA mutant population (An et al, 2005a(An et al, , 2005bJeong et al, 2006; http://www.postech.ac.kr/life/pfg/risd/index. html).…”

Section: Plant Growth Phenotype Of the Ossut2 Mutantmentioning

confidence: 99%

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Impaired Function of the Tonoplast-Localized Sucrose Transporter in Rice, OsSUT2, Limits the Transport of Vacuolar Reserve Sucrose and Affects Plant Growth

et al. 2011

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Physiological functions of sucrose (Suc) transporters (SUTs) localized to the tonoplast in higher plants are poorly understood. We here report the isolation and characterization of a mutation in the rice (Oryza sativa) OsSUT2 gene. Expression of OsSUT2-green fluorescent protein in rice revealed that OsSUT2 localizes to the tonoplast. Analysis of the OsSUT2 promoter::bglucuronidase transgenic rice indicated that this gene is highly expressed in leaf mesophyll cells, emerging lateral roots, pedicels of fertilized spikelets, and cross cell layers of seed coats. Results of Suc transport assays in yeast were consistent with a H + -Suc symport mechanism, suggesting that OsSUT2 functions in Suc uptake from the vacuole. The ossut2 mutant exhibited a growth retardation phenotype with a significant reduction in tiller number, plant height, 1,000-grain weight, and root dry weight compared with the controls, the wild type, and complemented transgenic lines. Analysis of primary carbon metabolites revealed that ossut2 accumulated more Suc, glucose, and fructose in the leaves than the controls. Further sugar export analysis of detached leaves indicated that ossut2 had a significantly decreased sugar export ability compared with the controls. These results suggest that OsSUT2 is involved in Suc transport across the tonoplast from the vacuole lumen to the cytosol in rice, playing an essential role in sugar export from the source leaves to sink organs.

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Section: Plant Growth Phenotype Of the Ossut2 Mutantmentioning

confidence: 99%

“…A T-DNA-tagged OsSUT2 mutant, ossut2, was identified by searching the rice T-DNA Insertion Sequence Database (An et al, 2005a(An et al, , 2005bJeong et al, 2006; http://www.postech.ac.kr/life/pfg/risd/index.html). A homozygous mutant (cv Hwayoung) was isolated by genomic DNA PCR analysis.…”

Section: Isolation Of the Ossut2 Mutantmentioning

confidence: 99%

Impaired Function of the Tonoplast-Localized Sucrose Transporter in Rice, OsSUT2, Limits the Transport of Vacuolar Reserve Sucrose and Affects Plant Growth

et al. 2011

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“…To confirm the role of OsRAR1 in the immune response in the rice plant, we isolated an osrar1 knockout mutant from our T-DNA mutant population (An et al, 2005a;2005b;Jeong et al, 2006). The isolated mutant allele, osrar1, harbors a T-DNA insertion in the fourth exon of OsRAR1 (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Dongjin) osrar1 was identified by searching the rice T-DNA Insertion Sequence Database (An et al, 2005a;2005b;Jeong et al, 2006; http://signal. salk.edu/cgi-bin/RiceGE).…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Differential Requirement of Oryza sativa RAR1 in Immune Receptor-Mediated Resistance of Rice to Magnaporthe oryzae

Song

Kim

Han

et al. 2013

Molecules and Cells

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The required for Mla12 resistance (RAR1) protein is essential for the plant immune response. In rice, a model monocot species, the function of Oryza sativa RAR1 (OsRAR1) has been little explored. In our current study, we characterized the response of a rice osrar1 T-DNA insertion mutant to infection by Magnaporthe oryzae, the causal agent of rice blast disease. osrar1 mutants displayed reduced resistance compared with wild type rice when inoculated with the normally virulent M. oryzae isolate PO6-6, indicating that OsRAR1 is required for an immune response to this pathogen. We also investigated the function of Os-RAR1 in the resistance mechanism mediated by the immune receptor genes Pib and Pi5 that encode nucleotide binding-leucine rich repeat (NB-LRR) proteins. We inoculated progeny from Pib/osrar1 and Pi5/osrar1 heterozygous plants with the avirulent M. oryzae isolates, race 007 and PO6-6, respectively. We found that only Pib-mediated resistance was compromised by the osrar1 mutation and that the introduction of the OsRAR1 cDNA into Pib/osrar1 rescued Pib-mediated resistance. These results indicate that OsRAR1 is required for Pib-mediated resistance but not Pi5-mediated resistance to M. oryzae.

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“…fr/OryzaTagLine/) [34], Japan (http://pfg101.nias.affrc. go.jp/~miyao/pub/tos17/index.html.en) [35,36], Korea (http://141.223.132.44/pfg/) [37,38] and USA (http:// sundarlab.ucdavis.edu/) [39]. These resources helped to identify and study genes involved in rice development [40,41].…”

Section: Fang-fang Fu Et Al 381mentioning

confidence: 99%

Studies on rice seed quality through analysis of a large-scale T-DNA insertion population

et al. 2009

Cell Res

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A rice (Oryza sativa) T-DNA insertion population, which included more than 63 000 independent transgenic lines and 8 840 identified flanking sequence tags (FSTs) that were mapped onto the rice genome, was developed to systemically study the rice seed quality control. Genome-wide analysis of the FST distribution showed that T-DNA insertions were positively correlated with expressed genes, but negatively with transposable elements and small RNAs. In addition, the recovered T-DNAs were preferentially located at the untranslated region of the expressed genes. More than 11 000 putative homozygous lines were obtained through multi-generations of planting and resistance screening, and measurement of seed quality of around half of them, including the contents of starch, amylose, protein and fat, with a nondestructive near-infrared spectroscopy method, identified 551 mutants with unique or multiple altered parameters of seed quality. Analysis of the corresponding FSTs showed that genes participating in diverse functions, including metabolic processes and transcriptional regulation, were involved, indicating that seed quality is regulated by a complex network. IntroductionRice (Oryza sativa), as a wholesome and nutritious cereal grain, provides the staple food for more than half of the world's population [1]. Along with the growth of economies and population throughout the world, there is an increasing demand for high-yield and high-quality rice. The grain quality of rice, including that of milling, appearance, cooking and eating, and nutrition, is determined by multiple physicochemical properties [2], and studies on the involved key genes and relevant regulatory mechanisms will help to illustrate the regulatory networks of rice quality control and benefit for the breeding efforts.Starch, which is composed of amylose and amylopectin, comprises ∼90% of dry endosperm of rice seed.The apparent amylose content (AAC) is recognized as an important determinant of the appearance and texture of grain [3]. Thai jasmine rice (KDML 105), one of the best-quality rice with good cooking and eating qualities, has low amylose content (AC) and medium gel consistency (GC) [4].The nutritional quality includes the proportions of protein, fat, mineral and other substances [5]. The rice protein is rich in methionine and cysteine, and compares favorably with proteins of other cereals, but is poor in lysine and threonine, making it an incomplete protein source for human infants [6]. Storage triacylglycerols are stored in oil bodies containing phospholipids and oleosins at the surface [7], and oil bodies are abundant in embryo and aleurone layer of rice seed. However, the aleurone layer is usually removed by milling because it turns rancid upon storage [8]. In addition, oil also influences other aspects of seed quality, and a recent report showed that the quality and viability of Arabidopsis seeds can be enhanced by suppressing phospholipase D [9].The near-infrared spectroscopy (NIRS) technology, which is based on the fact that several natural pr...

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Generation of a flanking sequence‐tag database for activation‐tagging lines in japonica rice

Cited by 315 publications

References 35 publications

Impaired Function of the Tonoplast-Localized Sucrose Transporter in Rice, OsSUT2, Limits the Transport of Vacuolar Reserve Sucrose and Affects Plant Growth

Impaired Function of the Tonoplast-Localized Sucrose Transporter in Rice, OsSUT2, Limits the Transport of Vacuolar Reserve Sucrose and Affects Plant Growth

Differential Requirement of Oryza sativa RAR1 in Immune Receptor-Mediated Resistance of Rice to Magnaporthe oryzae

Studies on rice seed quality through analysis of a large-scale T-DNA insertion population

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