Generation of a Knockout Mouse Embryonic Stem Cell Line Using a Paired CRISPR/Cas9 Genome Engineering Tool

Wettstein, Rahel; Bodak, Maxime; Ciaudo, Constance

doi:10.1007/7651_2015_213

Cited by 23 publications

(29 citation statements)

References 30 publications

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“…Independent mESC clones were isolated and genomic deletions were confirmed by PCR (Fig. B) . Immunobloting analysis validated the absence of DICER protein in both mutant mESC lines (Fig.…”

Section: Resultsmentioning

confidence: 80%

Dicer, a new regulator of pluripotency exit and LINE‐1 elements in mouse embryonic stem cells

Bodak

Cirera‐Salinas²,

et al. 2017

FEBS Open Bio

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A gene regulation network orchestrates processes ensuring the maintenance of cellular identity and genome integrity. Small RNA s generated by the RNA se III DICER have emerged as central players in this network. Moreover, deletion of Dicer in mice leads to early embryonic lethality. To better understand the underlying mechanisms leading to this phenotype, we generated Dicer ‐deficient mouse embryonic stem cells ( mESC s). Their detailed characterization revealed an impaired differentiation potential, and incapacity to exit from the pluripotency state. We also observed a strong accumulation of LINE ‐1 (L1s) transcripts, which was translated at protein level and led to an increased L1s retrotransposition. Our findings reveal Dicer as a new essential player that sustains mESC s self‐renewal and genome integrity by controlling L1s regulation.

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“…Independent mESC clones were isolated and genomic deletions were confirmed by PCR (Fig. B) . Immunobloting analysis validated the absence of DICER protein in both mutant mESC lines (Fig.…”

Section: Resultsmentioning

confidence: 80%

Dicer, a new regulator of pluripotency exit and LINE‐1 elements in mouse embryonic stem cells

Bodak

Cirera‐Salinas²,

et al. 2017

FEBS Open Bio

Self Cite

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“…CRISPR/Cas9 can create deletion models by producing embryonic stem cells exposed to NHEJ or single nucleotide mutation and gene insertion models via homology directed repair (An et al, 2016; Chen J. R. et al, 2016; Markossian and Flamant, 2016; Nakagawa et al, 2016; Wettstein et al, 2016). We have shown earlier that complexes formed from Cas9 and nucleic acid gRNA can be delivered to mouse inner hair cells directly (Zuris et al, 2015).…”

Section: Gene Editing Strategiesmentioning

confidence: 99%

Recent Advancements in the Regeneration of Auditory Hair Cells and Hearing Restoration

Mittal

Nguyen

Patel

et al. 2017

Front. Mol. Neurosci.

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Neurosensory responses of hearing and balance are mediated by receptors in specialized neuroepithelial sensory cells. Any disruption of the biochemical and molecular pathways that facilitate these responses can result in severe deficits, including hearing loss and vestibular dysfunction. Hearing is affected by both environmental and genetic factors, with impairment of auditory function being the most common neurosensory disorder affecting 1 in 500 newborns, as well as having an impact on the majority of elderly population. Damage to auditory sensory cells is not reversible, and if sufficient damage and cell death have taken place, the resultant deficit may lead to permanent deafness. Cochlear implants are considered to be one of the most successful and consistent treatments for deaf patients, but only offer limited recovery at the expense of loss of residual hearing. Recently there has been an increased interest in the auditory research community to explore the regeneration of mammalian auditory hair cells and restoration of their function. In this review article, we examine a variety of recent therapies, including genetic, stem cell and molecular therapies as well as discussing progress being made in genome editing strategies as applied to the restoration of hearing function.

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“…Mandal et al (2014) successfully silenced the expression of the genes B2M and CCR5 in human hematopoietic cells using CRISPR/Cas9 with minimal off-target mutagenesis. Additionally, Wettstein et al (2016) transfected two paired CRISPR single guide RNAs (sgRNAs)-Cas9 plasmids into mouse embryonic stem cells, which resulted in the knock-out of the targeted gene.…”

Section: Msc Therapy and Crispr/cas9mentioning

confidence: 99%

Current status and future prospects of mesenchymal stem cell therapy for liver fibrosis

Guo

Chen

et al. 2016

J. Zhejiang Univ. Sci. B

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Liver fibrosis is the end-stage of many chronic liver diseases and is a significant health threat. The only effective therapy is liver transplantation, which still has many problems, including the lack of donor sources, immunological rejection, and high surgery costs, among others. However, the use of cell therapy is becoming more prevalent, and mesenchymal stem cells (MSCs) seem to be a promising cell type for the treatment of liver fibrosis. MSCs have multiple differentiation abilities, allowing them to migrate directly into injured tissue and differentiate into hepatocyte-like cells. Additionally, MSCs can release various growth factors and cytokines to increase hepatocyte regeneration, regress liver fibrosis, and regulate inflammation and immune responses. In this review, we summarize the current uses of MSC therapies for liver fibrosis and suggest potential future applications.

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Generation of a Knockout Mouse Embryonic Stem Cell Line Using a Paired CRISPR/Cas9 Genome Engineering Tool

Cited by 23 publications

References 30 publications

Dicer, a new regulator of pluripotency exit and LINE‐1 elements in mouse embryonic stem cells

Dicer, a new regulator of pluripotency exit and LINE‐1 elements in mouse embryonic stem cells

Recent Advancements in the Regeneration of Auditory Hair Cells and Hearing Restoration

Current status and future prospects of mesenchymal stem cell therapy for liver fibrosis

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