Generation of a PDX1–EGFP reporter human induced pluripotent stem cell line, KSCBi005-A-3, using the CRISPR/Cas9 system

Lee, Youngsun; Choi, Hye Young; Kwon, Ara; Park, Hyeyeon; Park, Mi-Hyun; Kim, Jiwon; Kim, Min Jung; Kim, Yong-Ou; Kwak, Sungwook; Koo, Soo Kyung

doi:10.1016/j.scr.2019.101632

Cited by 9 publications

(5 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When these engineered iPSCs were differentiated into SC-β cells, EGFP expression was specifically detected in insulin-producing cells, but not in the definitive endoderm stage. This strategy holds promise for purifying mature β-cell therapeutics [43]. Yoshihara et al [44] employed CRIS-PR/Cas9 to knock in the GFP sequence downstream of the INS promoter, enabling the facile observation of insulin-producing cells in iPSC-derived islet-like organoids.…”

Section: Genome Editing To Overcome Challenges Of Current Sc-β Cellsmentioning

confidence: 99%

Cryopreservation of engineered tissues and organoids

Kim,

Lee

2023

Organoid

View full text Add to dashboard Cite

Although engineering transplantable tissues for clinical applications is difficult, cryopreserving those tissues to create pre-made engineered tissue for emergency transplantation is an even greater challenge. It remains uncertain whether the cryopreservation of engineered tissues will become a reality, but if so, it could have a significant impact on public health by saving lives and simplifying clinical treatment procedures. All in all, the potential implications of this innovation could have far-reaching consequences for the medical field as a whole. This review introduces the basic principles of cryopreservation techniques, including the procedures and components involved. Additionally, a list of its applications to engineered tissue is presented.

show abstract

Section: Genome Editing To Overcome Challenges Of Current Sc-β Cellsmentioning

confidence: 99%

Cryopreservation of engineered tissues and organoids

Kim,

Lee

2023

Organoid

View full text Add to dashboard Cite

show abstract

“…Lee et al [43] employed genome editing to address this challenge by generating an iPSC line expressing the pancreas/ duodenum homeobox protein 1 (PDX1)-enhanced green fluorescent protein (EGFP) fusion protein. PDX1 is a critical pancreatic β-cell marker essential for β-cell development and maturation [43]. Thus, functionally mature SC-β cells would express the PDX1-EGFP fusion protein.…”

Section: Genome Editing To Overcome Challenges Of Current Sc-β Cellsmentioning

confidence: 99%

“…Moreover, the engineered islets could be protected from The editing method allowed the facile isolation of insulinpositive β cells after differentiation. [43] Knock-in of a GFP sequence downstream of the INS promoter in iPSCs SpCas9-mediated knock-in by HDR Insulin-positive cells could be spotted from a cell mixture. [44] Knock-out of SLC30A8 in ESCs SpCas9-mediated knock-out ZnT8 loss-of-function improved GSIS in SC-β cells.…”

Section: Genome Editing In Primary Pancreatic Isletsmentioning

confidence: 99%

Genome editing for engineering stem cell-derived pancreatic β cells: recent trends and future perspectives

Jang,

Shin,

Jeong

et al. 2023

Organoid

View full text Add to dashboard Cite

show abstract

“…Antibodies suitable for such proteins are usually used for fluorescence imaging [ 99 ]. Table 1 summarizes the studies that have monitored the differentiation process of stem cells over the past three years using immunocytochemistry [ 95 , 98 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 , 115 , 116 , 117 , 118 , 119 , 120 , 121 , 122 , 123 , 124 , 125 , 126 , 127 , 128 , 129 , 130 , 131 , 132 , 133 , 134 , 135 , 136 ]. ICC is a non-destructive method, different from Western blot.…”

Section: Latest Technological Advances In Monitoring Stem Cell Status...mentioning

confidence: 99%

Recent Advances in Monitoring Stem Cell Status and Differentiation Using Nano-Biosensing Technologies

et al. 2022

View full text Add to dashboard Cite

In regenerative medicine, cell therapies using various stem cells have received attention as an alternative to overcome the limitations of existing therapeutic methods. Clinical applications of stem cells require the identification of characteristics at the single-cell level and continuous monitoring during expansion and differentiation. In this review, we recapitulate the application of various stem cells used in regenerative medicine and the latest technological advances in monitoring the differentiation process of stem cells. Single-cell RNA sequencing capable of profiling the expression of many genes at the single-cell level provides a new opportunity to analyze stem cell heterogeneity and to specify molecular markers related to the branching of differentiation lineages. However, this method is destructive and distorted. In addition, the differentiation process of a particular cell cannot be continuously tracked. Therefore, several spectroscopic methods have been developed to overcome these limitations. In particular, the application of Raman spectroscopy to measure the intrinsic vibration spectrum of molecules has been proposed as a powerful method that enables continuous monitoring of biochemical changes in the process of the differentiation of stem cells. This review provides a comprehensive overview of current analytical methods employed for stem cell engineering and future perspectives of nano-biosensing technologies as a platform for the in situ monitoring of stem cell status and differentiation.

show abstract

Generation of a PDX1–EGFP reporter human induced pluripotent stem cell line, KSCBi005-A-3, using the CRISPR/Cas9 system

Cited by 9 publications

References 5 publications

Cryopreservation of engineered tissues and organoids

Cryopreservation of engineered tissues and organoids

Genome editing for engineering stem cell-derived pancreatic β cells: recent trends and future perspectives

Recent Advances in Monitoring Stem Cell Status and Differentiation Using Nano-Biosensing Technologies

Contact Info

Product

Resources

About