Generation of a photothermally responsive antimicrobial, bioadhesive gelatin methacryloyl (GelMA) based hydrogel through 3D printing for infectious wound healing

Cheng, Feng; Xu, Lei; Zhang, Xiao; He, Jinmei; Huang, Yudong; Li, Hongbin

doi:10.1016/j.ijbiomac.2024.129372

Cited by 9 publications

(3 citation statements)

References 54 publications

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“…Even though sodium alginate-based hydrogel is a commonly utilized material for cell cultures, the inherent lack of cellular interaction properties in this polysaccharide often leads to its combination with other materials, such as gelatin or cellulose . Different works report on using graphene-based materials and alginate to prepare bioinks for wound dressing, − proving the enhanced printability and mechanical properties of the final scaffolds, however, without including cells into the ink formulation.…”

Section: Resultsmentioning

confidence: 99%

Light-Responsive and Antibacterial Graphenic Materials as a Holistic Approach to Tissue Engineering

Ferreras,

Matesanz,

Mendizabal

et al. 2024

ACS Nanosci. Au

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While the continuous development of advanced bioprinting technologies is under fervent study, enhancing the regenerative potential of hydrogel-based constructs using external stimuli for wound dressing has yet to be tackled. Fibroblasts play a significant role in wound healing and tissue implants at different stages, including extracellular matrix production, collagen synthesis, and wound and tissue remodeling. This study explores the synergistic interplay between photothermal activity and nanomaterial-mediated cell proliferation. The use of different graphene-based materials (GBM) in the development of photoactive bioinks is investigated. In particular, we report the creation of a skin-inspired dressing for wound healing and regenerative medicine. Three distinct GBM, namely, graphene oxide (GO), reduced graphene oxide (rGO), and graphene platelets (GP), were rigorously characterized, and their photothermal capabilities were elucidated. Our investigations revealed that rGO exhibited the highest photothermal efficiency and antibacterial properties when irradiated, even at a concentration as low as 0.05 mg/mL, without compromising human fibroblast viability. Alginate-based bioinks alongside human fibroblasts were employed for the bioprinting with rGO. The scaffold did not affect the survival of fibroblasts for 3 days after bioprinting, as cell viability was not affected. Remarkably, the inclusion of rGO did not compromise the printability of the hydrogel, ensuring the successful fabrication of complex constructs. Furthermore, the presence of rGO in the final scaffold continued to provide the benefits of photothermal antimicrobial therapy without detrimentally affecting fibroblast growth. This outcome underscores the potential of rGO-enhanced hydrogels in tissue engineering and regenerative medicine applications. Our findings hold promise for developing game-changer strategies in 4D bioprinting to create smart and functional tissue constructs with high fibroblast proliferation and promising therapeutic capabilities in drug delivery and bactericidal skin-inspired dressings.

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Section: Resultsmentioning

confidence: 99%

Light-Responsive and Antibacterial Graphenic Materials as a Holistic Approach to Tissue Engineering

Ferreras,

Matesanz,

Mendizabal

et al. 2024

ACS Nanosci. Au

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“…After measuring dry weights, wet weights were determined at different time points (i.e., 1 h, 2 h, 4 h, 8 h, 12 h, 24 h, 48 h) by gentle blotting with filter papers to remove exceeding surface liquid. The swelling ratio was calculated according to Equation (1) [21]:…”

Section: Evaluation Of the Swelling Behaviormentioning

confidence: 99%

“…Among various photothermal materials, polydopamine-functionalized graphene oxide (rGO@PDA) exhibits excellent thermal stability, high photothermal conversion capability, and superior biocompatibility, which is suitable for photothermal antibacterial wound therapy [17][18][19][20]. For example, photothermally responsive antimicrobial gelatin methacryloyl hydrogels have been fabricated for the treatment of wound infections based on rGO@PDA [21].…”

Section: Introductionmentioning

confidence: 99%

Cotton Fabric-Reinforced Hydrogels with Excellent Mechanical and Broad-Spectrum Photothermal Antibacterial Properties

Yuan,

Zhang,

Shi

et al. 2024

Polymers

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Antibacterial hydrogel wound dressings hold great potential in eliminating bacteria and accelerating the healing process. However, it remains a challenge to fabricate hydrogel wound dressings that simultaneously exhibit excellent mechanical and photothermal antibacterial properties. Here we report the development of polydopamine-functionalized graphene oxide (rGO@PDA)/calcium alginate (CA)/Polypyrrole (PPy) cotton fabric-reinforced hydrogels (abbreviated as rGO@PDA/CA/PPy FHs) for tackling bacterial infections. The mechanical properties of hydrogels were greatly enhanced by cotton fabric reinforcement and an interpenetrating structure, while excellent broad-spectrum photothermal antibacterial properties based on the photothermal effect were obtained by incorporating PPy and rGO@PDA. Results indicated that rGO@PDA/CA/PPy FHs exhibited superior tensile strength in both the warp (289 ± 62.1 N) and weft directions (142 ± 23.0 N), similarly to cotton fabric. By incorporating PPy and rGO@PDA, the swelling ratio was significantly decreased from 673.5% to 236.6%, while photothermal conversion performance was significantly enhanced with a temperature elevated to 45.0 °C. Due to the synergistic photothermal properties of rGO@PDA and PPy, rGO@PDA/CA/PPy FHs exhibited excellent bacteria-eliminating efficiency for S. aureus (0.57%) and E. coli (3.58%) after exposure to NIR for 20 min. We believe that the design of fabric-reinforced hydrogels could serve as a guideline for developing hydrogel wound dressings with improved mechanical properties and broad-spectrum photothermal antibacterial properties for infected-wound treatment.

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3D Printing‐Based Hydrogel Dressings for Wound Healing

Zhou,

Yu,

You

et al. 2024

Advanced Science

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Skin wounds have become an important issue that affects human health and burdens global medical care. Hydrogel materials similar to the natural extracellular matrix (ECM) are one of the best candidates for ideal wound dressings and the most feasible choices for printing inks. Distinct from hydrogels made by traditional technologies, which lack bionic and mechanical properties, 3D printing can promptly and accurately create hydrogels with complex bioactive structures and the potential to promote tissue regeneration and wound healing. Herein, a comprehensive review of multi‐functional 3D printing‐based hydrogel dressings for wound healing is presented. The review first summarizes the 3D printing techniques for wound hydrogel dressings, including photo‐curing, extrusion, inkjet, and laser‐assisted 3D printing. Then, the properties and design approaches of a series of bioinks composed of natural, synthetic, and composite polymers for 3D printing wound hydrogel dressings are described. Thereafter, the application of multi‐functional 3D printing‐based hydrogel dressings in a variety of wound environments is discussed in depth, including hemostasis, anti‐inflammation, antibacterial, skin appendage regeneration, intelligent monitoring, and machine learning‐assisted therapy. Finally, the challenges and prospects of 3D printing‐based hydrogel dressings for wound healing are presented.

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Generation of a photothermally responsive antimicrobial, bioadhesive gelatin methacryloyl (GelMA) based hydrogel through 3D printing for infectious wound healing

Cited by 9 publications

References 54 publications

Light-Responsive and Antibacterial Graphenic Materials as a Holistic Approach to Tissue Engineering

Light-Responsive and Antibacterial Graphenic Materials as a Holistic Approach to Tissue Engineering

Cotton Fabric-Reinforced Hydrogels with Excellent Mechanical and Broad-Spectrum Photothermal Antibacterial Properties

3D Printing‐Based Hydrogel Dressings for Wound Healing

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