2000
DOI: 10.1128/iai.68.12.6777-6784.2000
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Generation of a Recombinant 65-Kilodalton Mannoprotein, a Major Antigen Target of Cell-Mediated Immune Response toCandida albicans

Abstract: ؉ T-cell clones were generated using a C. albicans mannoprotein fraction as an antigenic stimulant. Several of these clones specifically responded to both the native and the recombinant C. albicans Mp65 but not to ScMp65. Thus, the recombinant Mp65 of C. albicans retains antigenicity and, as such, could be a valid, standardized reagent for serodiagnostic and immunological studies.

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Cited by 47 publications
(94 citation statements)
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“…Another group of kexin-processed substrates, the SAPs, play a role in adhesion to epithelial cells (61). Cell wall mannoprotein Mp65, which experimentally is processed at a consensus kexin cleavage site, probably contributes to adhesion as it has a complement-binding RGD site not found in its S. cerevisiae homologue ScScw10p (62). The hyphal wall protein Hwp1p, on the other hand, has been shown to be dispensable for attachment of the organism to endothelial cells (47).…”
Section: Discussionmentioning
confidence: 99%
“…Another group of kexin-processed substrates, the SAPs, play a role in adhesion to epithelial cells (61). Cell wall mannoprotein Mp65, which experimentally is processed at a consensus kexin cleavage site, probably contributes to adhesion as it has a complement-binding RGD site not found in its S. cerevisiae homologue ScScw10p (62). The hyphal wall protein Hwp1p, on the other hand, has been shown to be dispensable for attachment of the organism to endothelial cells (47).…”
Section: Discussionmentioning
confidence: 99%
“…Our data, in particular the strong reaction of MAb 7H6 with a 75-kDa constituent of C. neoformans extract, may also imply that CnMP is a degradation product of this or another polydisperse MAb-reactive extract or secretory material of C. neoformans. Studies of the molecular cloning of the MP65-encoding gene of C. albicans are in progress (12).…”
Section: Discussionmentioning
confidence: 99%
“…A homologue of Scw10p in Candida albicans CaMP65 was characterized as a major immunodominant antigen. This 65 kDa mannoprotein is particularly observed in extracellular fractions of hyphae (La Valle et al, 2000).…”
Section: Introductionmentioning
confidence: 98%