Generation of a RRAGA knockout human iPSC line GIBHi002-A-5 using CRISPR/Cas9 technology

Sun, Yilu; Fu, Jian; Yang, Jiayin; Zhao, Jia

doi:10.1016/j.scr.2022.102859

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What Do Psc-derived Brain Cells Bring To Psychedelic Studies?1

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2024

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Cited by 3 publications

(1 citation statement)

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“… 81 PSCs derived from healthy donors can have disease-associated mutations inserted into relevant genetic loci, providing a reliable model for studying specific transducers’ impact on resulting phenotypic responses. 82 …”

Section: What Do Psc-derived Brain Cells Bring To Psychedelic Studies?mentioning

confidence: 99%

Human pluripotent stem cells as a translational toolkit in psychedelic research in vitro

Salerno,

Rehen

2024

iScience

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Section: What Do Psc-derived Brain Cells Bring To Psychedelic Studies?mentioning

confidence: 99%

Human pluripotent stem cells as a translational toolkit in psychedelic research in vitro

Salerno,

Rehen

2024

iScience

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Vector enabled CRISPR gene editing – A revolutionary strategy for targeting the diversity of brain pathologies

Forgham

Liu

Zhu

et al. 2023

Coordination Chemistry Reviews

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Induced Pluripotent Stem Cells in the Era of Precise Genome Editing

Punetha

Saini

Chaudhary

et al. 2024

CSCR

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Genome editing has enhanced our ability to understand the role of genetics in a number of diseases by facilitating the development of more precise cellular and animal models to study pathophysiological processes. These advances have shown extraordinary promise in a multitude of areas, from basic research to applied bioengineering and biomedical research. Induced pluripotent stem cells (iPSCs) are known for their high replicative capacity and are excellent targets for genetic manipulation as they can be clonally expanded from a single cell without compromising their pluripotency. Clustered, regularly interspaced short palindromic repeats (CRISPR) and CRISPR/Cas RNA-guided nucleases have rapidly become the method of choice for gene editing due to their high specificity, simplicity, low cost, and versatility. Coupling the cellular versatility of iPSCs differentiation with CRISPR/Cas9-mediated genome editing technology can be an effective experimental technique for providing new insights into the therapeutic use of this technology. However, before using these techniques for gene therapy, their therapeutic safety and efficacy following models need to be assessed. In this review, we cover the remarkable progress that has been made in the use of genome editing tools in iPSCs, their applications in disease research and gene therapy as well as the hurdles that remain in the actual implementation of CRISPR/Cas systems.

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Generation of a RRAGA knockout human iPSC line GIBHi002-A-5 using CRISPR/Cas9 technology

Cited by 3 publications

References 3 publications

Human pluripotent stem cells as a translational toolkit in psychedelic research in vitro

Human pluripotent stem cells as a translational toolkit in psychedelic research in vitro

Vector enabled CRISPR gene editing – A revolutionary strategy for targeting the diversity of brain pathologies

Induced Pluripotent Stem Cells in the Era of Precise Genome Editing

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