Generation of a Specific Polyclonal Antibody With High Affinity to Atorvastatin and Its Employment in the Development of Elisa for Determination of Atorvastatin in Plasma

Abstract

For the first time, a polyclonal antibody with high affinity to atorvastatin (ATR) was generated. The high specificity of the antibody for ATR among its structural analogues and co-administered therapeutic agents was proved. The antibody was employed in the development of enzyme-linked immunosorbent assay for quantitation of ATR in plasma. The assay was validated over a working range of 0.2-5 ng/mL. The intra- and interassay precisions were satisfactory; the coefficients of variations were ≤5%. The accuracy of the method was proved as the mean recovery was 96.4 ± 4.3%. The assay can be used in therapeutic monitoring and pharmacokinetic studies for ATR.

Construction and operation of a microrobot based on magnetotactic bacteria in a microfluidic chip

“…The intervals for each immunization is 2 weeks. 44 Two weeks after the last immunization, the antiserum was collected. The sensitivity of antibodies was tested by slide agglutination method and the results showed that the polyclonal antibody could agglutinate with MO-1 at the minimum concentration of 1:640.…”