2011
DOI: 10.1021/bi201005g
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Generation of Autonomous Activity of Ca2+/Calmodulin-Dependent Protein Kinase Kinase β by Autophosphorylation

Abstract: Ca(2+)/calmodulin-dependent protein kinase kinases (CaMKKs) phosphorylate and activate specific downstream protein kinases, including CaMKI, CaMKIV, and 5'-AMP-activated protein kinase, which mediates a variety of Ca(2+) signaling cascades. CaMKKs have been shown to undergo autophosphorylation, although their role in enzymatic regulation remains unclear. Here, we found that CaMKKα and β isoforms expressed in nonstimulated transfected COS-7 cells, as well as recombinant CaMKKs expressed in and purified from Esc… Show more

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Cited by 29 publications
(30 citation statements)
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“…33). PS can increase expression of CaMKKβ through an augmentation of CaMKKβ stability from autophosphorylation (34,35), as well as an increase of CaMKKβ transcription, as shown in the current study, both in the flow channel in vitro and in the thoracic aorta in vivo (Fig. S1 C and D).…”
Section: Discussionsupporting
confidence: 79%
“…33). PS can increase expression of CaMKKβ through an augmentation of CaMKKβ stability from autophosphorylation (34,35), as well as an increase of CaMKKβ transcription, as shown in the current study, both in the flow channel in vitro and in the thoracic aorta in vivo (Fig. S1 C and D).…”
Section: Discussionsupporting
confidence: 79%
“…MALDI‐TOF MS was performed on an AXIMA‐TOF 2 instrument (Shimadzu, Kyoto, Japan) in the positive reflection mode. In order to identify the amino acid sequences of the major peptides, an LC‐MS/MS analysis was performed on an LCMS‐IT‐TOF instrument (Shimadzu) interfaced with a nano reverse‐phase LC system (Shimadzu) . The MS/MS data were acquired in the datum‐dependent mode using the LCMS solution software program (Shimadzu) and converted to a single text file (containing the observed precursor peptide m / z , fragment ion m / z , and intensity values) with Mascot Distiller (Matrix Science, Boston, MA, USA).…”
Section: Methodsmentioning
confidence: 99%
“…CaMKK2 protein is also subject to numerous post-translational modifications ( Fig. 1) that affect protein stability and activity (27,28). The cAMP/PKA pathway can inhibit CaMKK2 activity, and other inhibitory signals await discovery (81).…”
Section: Concluding Remarks and Perspectivesmentioning
confidence: 99%