Generation of Backbone-Free, Low Transgene Copy Plants by Launching T-DNA from the<i>Agrobacterium</i>Chromosome

Oltmanns, Heiko; Frame, Bronwyn; Lee, Lan‐Ying; Johnson, Susan; Li, Bo; Wang, Kan; Gelvin, Stanton B.

doi:10.1104/pp.109.148585

Cited by 77 publications

(57 citation statements)

References 48 publications

(60 reference statements)

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“…A recent study has shown that launching T-DNA from the plant-inducible picA locus in the A . tumefaciens chromosome was found to reduce transgene copy number and almost eliminate the presence of T-DNA backbone sequences in Arabidopsis and maize (Oltmanns et al 2010 ) . Such a strategy may enable transgenic sugarcane plants produced by Agrobacterium -mediated transformation to exhibit higher levels of transgene expression but also free of residual antibiotic resistance genes and backbone sequences.…”

Section: Availability Of New Transformation Vectorsmentioning

confidence: 99%

Genetic Engineering of Saccharum

Beyene

Curtis

Damaj

et al. 2012

Genomics of the Saccharinae

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Over the last two decades, substantial progress has been made in the genetic engineering of sugarcane ( Saccharum spp.) through improvements in tissue culture procedures, allowing a higher ef fi ciency of generating transgenic plants using Agrobacterium -mediated and biolistic gene transfers. Elucidation of gene function and development of varieties with improved yield, sugar level, fi ber content, and other desirable traits and products for superior performance have been possible through transgenic technologies. Researchers are now focusing on optimizing existing methodologies and developing new technologies for the production of elite varieties, enhancement of transgene expression, and manipulation of metabolic pathways for improved molecular breeding and commercial exploitation. At present, no transgenic sugarcane has been released to the commercial market, but with the aid of large investments from the private sector, the commercialization of this major sugar-and biomass-producing crop should be accelerated.

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Section: Availability Of New Transformation Vectorsmentioning

confidence: 99%

Genetic Engineering of Saccharum

Beyene

Curtis

Damaj

et al. 2012

Genomics of the Saccharinae

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“…The Agrobacteriummediated method also generates transgenic events that carry DNA fragments outside the T-DNA region (vector backbones) (Shou et al, 2004), which is an undesired effect. To prevent extra DNA segments from being inserted into the genome, some researchers used purified DNA fragments that carry only the cassette with the gene of interest for bombardment (Breitler et al, 2002;Loc et al, 2002), whereas others devised multiple T-DNA borders (Kuraya et al, 2004) or launched T-DNA from the Agrobacterium chromosome (Oltmanns et al, 2010) frequency. In the future, both methods will probably be needed to maximize the genetic transformation of plants.…”

Section: Resultsmentioning

confidence: 99%

Genetic transformation of major cereal crops

Ji¹,

Xu²,

Wang³

2013

Int. J. Dev. Biol.

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Of the more than 50,000 edible plant species in the world, at least 10,000 species are cereal grains. Three major cereal crops, rice (Oryza sativa), maize (Zea mays), and wheat (Triticum sp.), provide two-thirds of the world's food energy intake. Although crop yields have improved tremendously thanks to technological advances in the past 50 years, population increases and climate changes continue to threaten the sustainability of current crop productions. Whereas conventional and marker-assisted breeding programs continue to play a major role in crop improvement, genetic engineering has drawn an intense worldwide interest from the scientific community. In the past decade, genetic transformation technologies have revolutionized agricultural practices and millions of hectares of biotech crops have been cultured. Because of its unique ability to insert well-characterized gene sequences into the plant genome, genetic engineering can also provide effective tools to address fundamental biological questions. This technology is expected to continue to be an indispensable approach for both basic and applied research. Here, we overview briefly the development of the genetic transformation in the top seven cereals, namely maize, rice, wheat, barley (Hordeum vulgare), sorghum (Sorghum sp.), oat (Avena sativa), and millets. The advantages and disadvantages of the two major transformation methods, Agrobacterium tumefaciens-mediated and biolistic methods, are also discussed.

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“…Transgene integration in the plant genome is a complex process. Generally, Agrobacterium-mediated genetic transformation produces transgenic lines with a relatively low (1-3) transgene copy number (Kohli et al 2003;Olhoft et al 2004;Oltamanns et al 2010;Fladung et al 2013). Particle bombardment transformation method, on the other hand, typically integrates on average higher (1-10 or even up to 100) transgene copy number and complex integration in the genome (Svitashev and Somers 2001;Makarevitch et al 2003;Kohli et al 2003).…”

Section: Gene Targeting and Genome Editingmentioning

confidence: 98%

Next Generation Plant Biotechnology

Ahuja¹

2014

Sustainable Development and Biodiversity

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Modern plant biotechnology began with the transfer of foreign chimeric genes into plants. Initially recombinant genes were derived from bacteria, animals and plants for gene transfer. Gene transfer was accomplished by Agrobacteriummediated or biolistic methods into the plant genome. The first wave of transgenic plants that were monitored for transgene integration and expression, the second wave transgenic plants carried economically important genes for herbicide tolerance, pest resistance, drought and salt tolerance, growth traits, and flowering control. Subsequently, a number of genetically modified crops with several useful traits have been commercialized. Although relatively stable transgene expression has been observed in a number of plant species, there were also unintended unstable events in transgenic plants. This is due to the fact that transgene integration achieved by the two traditional methods ( Agrobacterium or biolistic) of gene transfer in the plant genome is random, and one to several copies of the transgenes may be integrated at one or several locations in the genome. In order to overcome the problem of randomness of transgene integration, site-specific transgene integration strategies have been experimentally tested in plants, and offer prospects of stable gene integration and expression in transgenic plants. In order to broaden the scope of transgenic plants, biotechnologists started looking for other useful avenues for their utility. With finite reserves of fossil fuels and climate change, and growing demands for fuels, plastics, and pharmaceuticals, transgenic plants have been also explored as production platforms for these commodities. This paper is an overview of next generation transgenic plants that can serve as bioreactors or biofactories for the cost-effective production of biofuels, biopharmaceuticals, bioplastics, and as a resource for nutritional supplements to meet human demands in the future. New developments in nanobiotechnology offer prospects for improved production of crop plants.

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Generation of Backbone-Free, Low Transgene Copy Plants by Launching T-DNA from theAgrobacteriumChromosome

Cited by 77 publications

References 48 publications

Genetic Engineering of Saccharum

Genetic Engineering of Saccharum

Genetic transformation of major cereal crops

Next Generation Plant Biotechnology

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