Generation of cytolytic T lymphocytes after reovirus infection: role of S1 gene.

Abstract

Cytotoxic T lymphocytes (CTLs) can be generated if spleen cells from reovirus-infected mice are stimulated in vitro with syngeneic reovirus-infected cells. These cytolytic effector cells demonstrate: (i) serotype specificity (i.e., maximal cytolytic activity is observed on target cells infected with the serotype used to induce the CTLs) and (ii) H-2 restriction. The

Experimental intestinal reovirus infection of mice

“…Finberg and colleagues provided the first evidence that MHC-restricted, reovirus-specific cytotoxic T lymphocytes (CTL) generated from reovirus-infected mice infected with reovirus serotypes 1 or 3 [8], and to demonstrate that anti-reovirus CTL respond to the σ1 hemagglutinin [8]. Subsequent studies have confirmed a role for CTL in the anti-reovirus immune response [42,[51][52][53][54].…”

“…The outer capsid consists of both structural and non-structural polypeptides [3,4]. The σ1 hemagglutinin, a structural capsid molecule, has been linked to many virus associated activities, including host cell attachment, viral neutralization by antibody, recognition by cytotoxic T cells, and tissue tropism and pathogenesis of reovirusmediated diseases [5][6][7][8][9][10][11][12][13][14][15][16]. Core proteins consist of transcriptases and replicases used in viral replication.…”

Mice immunogenicity after vaccination by DNA vaccines containing individual genes of a new type of reovirus

“…The outer capsid contains 3 polypeptides, μ1, σl, and σ3, which are encoded by genes of M2, S1, and S4, respectively (Weiner et al, 1978). The σl polypeptide is the viral hemagglutinin and has been linked to host cell attachment, type-specific neutralization, cytotoxic T cell recognition, tissue tropism, and pathogenesis of reovirus-mediated diseases (Finberg et al, 1979(Finberg et al, , 1982Lee et al, 1981;Weiner et al, 1977). Viral nonstructural proteins μNS (encoded by M3) and σNS (encoded by S3), as well as core protein μ2 (encoded by M1) play key roles in forming viral inclusions and recruiting other viral proteins and RNA to these structures for replication and assembly (Becker et al, 2001Broering et al, 2004;Mbisa et al, 2000;Parker et al, 2002).…”

The STRUCTURE AND FUNCTION OF THE REOVIRUS GENOME

“…It is also the reovirus hemagglutinin (119) and elicits the formation ofneutralizing antibody (116). It is responsible for the development of delayed hypersensitivity (117) and for the generation of suppressor T cells (19) and of cytolytic T lymphocytes (18). It also determines the extent to which reovirus particles interact with microtubules (5) and specifies reovirus tissue tropism and virulence (115) (see below).…”