Generation of Dopamine Transporter (DAT)-mCherry Knock-in Rats by CRISPR-Cas9 Genome Editing

Matsumoto, Nobuyoshi; Miyano, Miyuki; Abe, Takaya; Kashima, Tetsuhiko; Kato-Ishikura, Eriko; Inoue, Ken-ichi; Liu, Jiayan; Kiyonari, Hiroshi; Takeuchi, Haruki; Ikegaya, Yuji

doi:10.1248/bpb.b23-00598

Cited by 4 publications

(1 citation statement)

References 20 publications

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“…Genotyping was performed in accordance with a routine procedure established previously 10 . Small tissues obtained from the earlobes of mice were lysed with proteinase K, and genomic DNA was extracted and purified by a spin column method using the DNeasy Blood & Tissue Kit (69504, QIAGEN, Osaka, Japan) according to the manufacturer’s protocol.…”

Section: Methodsmentioning

confidence: 99%

Lengthened circadian rhythms in mice with self-controlled ambient light intensity

Ogasawara,

Matsumoto,

Takeuchi

et al. 2024

Sci Rep

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Laboratory animals are typically maintained under 12-h light and 12-h dark (12:12 LD) conditions with a daytime light intensity of ~ 200 lx. In this study, we designed an apparatus that allowed mice to self-select the room light intensity by nose poking. We measured the behavioral rhythms of the mice under this self-controlled light regimen. The mice quickly learned the relationship between their nose pokes and the resulting changes in the light intensity. Under these conditions, the mice exhibited free-running circadian behavior with a period of 24.5 ± 0.4 h. This circadian period was ~ 1 h longer than that of the same strain of mice when they were kept in constant darkness (DD) after 12:12 LD entrainment, and the lengthened period lasted for at least 30 days. The rhythm of the light intensity controlled by the mice also exhibited a similar period, but the phase of the illuminance rhythm preceded the phase of the locomotor activity rhythm. Mice that did not have access to the light controller were also entrained to the illuminance cycle produced by the mice that did have access to the light controller, but with a slightly delayed phase. The rhythm was likely controlled by the canonical circadian clock because mice with tau mutations in the circadian clock gene CSNK1E exhibited short periods of circadian rhythm under the same conditions. These results indicate that the free-running period of mice in the wild may differ from what they exhibit if they are attuned by forced light cycles in laboratories because mice in their natural habitats can self-control their exposure to ambient light, similar to our experimental conditions.

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Section: Methodsmentioning

confidence: 99%

Lengthened circadian rhythms in mice with self-controlled ambient light intensity

Ogasawara,

Matsumoto,

Takeuchi

et al. 2024

Sci Rep

Self Cite

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Dopamine-induced relaxation of spike synchrony diversifies burst patterns in cultured hippocampal networks

Hoang,

Matsumoto,

Miyano

et al. 2025

Neural Networks

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Dopamine-induced Relaxation of Connectivity Diversifies Burst Patterns in Cultured Hippocampal Networks

Hoang,

Matsumoto,

Miyano

et al. 2024

Preprint

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The intricate interplay of neurotransmitters orchestrates a symphony of neural activity in the hippocampus, with dopamine emerging as a key conductor in this complex ensemble. Despite numerous studies uncovering the cellular mechanisms of dopamine, its influence on hippocampal neural networks remains elusive. Combining in vitro electrophysiological recordings of rat embryonic hippocampal neurons, pharmacological interventions, and computational analyses of spike trains, we found that dopamine induces a relaxation in network connectivity, characterised by a reduction in spike coherence. This relaxation expands the repertoire of burst dynamics within these hippocampal networks, a phenomenon notably absent under the administration of dopamine antagonists. Our study provides a thorough understanding of the roles of dopamine signalling in shaping functional networks of hippocampal neurons.

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Generation of Dopamine Transporter (DAT)-mCherry Knock-in Rats by CRISPR-Cas9 Genome Editing

Cited by 4 publications

References 20 publications

Lengthened circadian rhythms in mice with self-controlled ambient light intensity

Lengthened circadian rhythms in mice with self-controlled ambient light intensity

Dopamine-induced relaxation of spike synchrony diversifies burst patterns in cultured hippocampal networks

Dopamine-induced Relaxation of Connectivity Diversifies Burst Patterns in Cultured Hippocampal Networks

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