Generation of double-layered equine mesenchymal stromal cell-derived osteochondral constructs

Abstract

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TRPV4 activation enhances compressive properties and glycosaminoglycan deposition of equine neocartilage sheets

“…Although the increase in mechanical properties was modest, chemical enhancement can represent an adjuvant to other strategies such as mechanical stimulation for functional tissue engineering of hyaline-like neocartilage with abundant presence of GAG and type-II collagen and low presence of type-I collagen (fibrocartilage). Recently characterized double-layered MSC-derived osteochondral constructs [ 54 ] show comparable mechanical properties than those of non-weight bearing regions of the joint. The combinatory approach of enhancing such osteochondral construct with TRPV4 stimulation seems a promising strategy for achieving properties similar to native benchmarks.…”