Generation of ESTs from grape berry at various developmental stages

Terrier, Nancy; Ageorges, Agnès; Abbal, Philippe; Romieu, Charles

doi:10.1078/0176-1617-00566

Cited by 48 publications

(31 citation statements)

References 33 publications

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“…This method yielded a list of clones called GRIP (grape ripening induced proteins), which become prevalent during ripening, but whose function remains obscure. The prevalence of these GRIPs was confirmed at the very beginning of our grape EST sequencing project (Terrier et al 2001b).…”

Section: Introductionmentioning

confidence: 58%

Isogene specific oligo arrays reveal multifaceted changes in gene expression during grape berry (Vitis vinifera L.) development

Terrier¹,

Glissant

Grimplet³

et al. 2005

Planta

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The transition from a green, hard, and acidic pericarp to a sweet, soft, coloured, and sugar-rich ripe fruit occurs in many unrelated fruit species. High throughput identification of differentially expressed genes in grape berry has been achieved by the use of 50-mers oligoarrays bearing a set of 3,200 Unigenes from Vitis vinifera to compare berry transcriptome at nine developmental stages. Analysis of transcript profiles revealed that most activations were triggered simultaneously with softening, occurring within only 24 h for an individual berry, just before any change in colouration or water, sugar, and acid content can be detected. Although most dramatically induced genes belong to unknown functional categories, numerous changes occur in the expression of isogenes involved in primary and secondary metabolism during ripening. Focusing on isogenes potentially significant in development regulation (hormonal control of transcription factor) revealed a possible role for several hormones (cytokinin, gibberellin, or jasmonic acid). Transcription factor analysis revealed the induction of RAP2 and WRKY genes at véraison, suggesting increasing biotic and abiotic stress conditions during ripening. This observation was strengthened by an increased expression of multiple transcripts involved in sugar metabolism and also described as induced in other plant organs during stress conditions. This approach permitted the identification of new isogenes as possible control points: a glutathione S-transferase exhibits the same expression profile as anthocyanin accumulation and a new putative sugar transporter is induced in parallel with sugar import.

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Section: Introductionmentioning

confidence: 58%

Isogene specific oligo arrays reveal multifaceted changes in gene expression during grape berry (Vitis vinifera L.) development

Terrier¹,

Glissant

Grimplet³

et al. 2005

Planta

Self Cite

249

218

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“…Identifying efficient strategies for conversion of cDNA sequences to the PCR-based assay for genetic analysis in Vitis is desirable to facilitate exploitation of emerging grape EST data Terrier et al 2001). ESTs will be particularly important for QTL mapping, because markers such as AFLPs, SSRs and RAPDs may predominantly target non-coding regions.…”

Section: Discussionmentioning

confidence: 99%

Molecular linkage maps of Vitis vinifera L. and Vitis riparia Mchx

Grando

Bellin

Edwards³

et al. 2002

Theor Appl Genet

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Two linkage maps for grape (Vitis spp.) have been developed based on 81 F(1) plants derived from an interspecific cross between the wine cultivar Moscato bianco (Vitis vinifera L.) and a Vitis riparia Mchx. accession, a donor of pathogen resistance traits. The double pseudotest-cross mapping strategy was applied using three types of molecular markers. The efficiency of SSRs to anchor homologous linkage groups from different Vitis maps and the usefulness of AFLPs in saturating molecular linkage maps were evaluated. Moreover, the SSCP technique was developed based on sequence information in public databases concerning genes involved in flavonoid and stilbene biosynthesis. For the maternal genetic map a total of 338 markers were assembled in 20 linkage groups covering 1,639 cM, whereas 429 loci defined the 19 linkage groups of the paternal map which covers 1,518 cM. The identification of 14 linkage groups common to both maps was possible based on 21 SSR and 19 AFLP loci. The position of SSR loci in the maps presented here was consistent with other published mapping experiments in Vitis.

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“…However, there is also 25.63% of the analyzed sequences which did not match any sequences in database after the sequencing analysis. Terrier et al (2001) found that the proportion of unmatched sequences was the highest at the veraison. Veraison is the onset of ripening, so some of these new genes encoding certain specific proteins could actually play an important role at the transient period which need further analysis to address their functions.…”

Section: Discussionmentioning

confidence: 99%

“…The grapevine is considered to be a non-climacteric fruit, and follows the trace of growth of a double-sigmoid curve. The two successive phases both last around 6 weeks with similar amplitudes (Terrier et al, 2001). After fruit set, cell divisions and expansion result in the rapid growth of the berry.…”

Section: Introductionmentioning

confidence: 99%

“…The chlorophyll breaks down and the color starts to turn. Certain secondary compounds involved with fruit flavor and aroma are formed (Coombe, 1992;Fillion et al, 1999;Davies and Robinson, 2000;Terrier et al, 2001 useful and efficient tools in function genomics and comparative genomic studies. The worldwide grape EST projects were initiated several years ago and made a significant progress so far.…”

Section: Introductionmentioning

confidence: 99%

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Generation and Analysis of ESTs in <i>V. amurensis</i> Grapevine Pericarp at Veraison

Ji¹,

Ли²,

Zhang³

et al. 2012

mpb

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In this study, we constructed the cDNA library of grape berry pericarp at veraison from wine grape 'Shuang Feng' (V. amurensis), and generated total 974 ESTs, representing the independent cDNA in length of around 392 672 bp, of which 710 unigenes were clustered into 97 contigs and 613 singletons based on quality of EST sequence and optimum assembly. 64.65% clones exhibited high homology to previously deposited nucleotides and/or polypeptide sequences, while the proportion of ESTs with no any significant homology in database reached 25.63%. Analysis of the unigenes resulted in the assignment of 9 specific functional classes in this research. Putative functions were identified for about 74.13% of the unigenes. By assigning them to specific function classes, we were able to highlight characteristics among different ripening phases. GO classification was performed to define the relationship among genes expressing in pericarp of wine grape berry, which were involved in three aspects including cell component, biological process and molecular function. Expression analysis of these ESTs were used to characterize potential roles of novel genes with respect to berry ripening and composition of wine grape.

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Generation of ESTs from grape berry at various developmental stages

Cited by 48 publications

References 33 publications

Isogene specific oligo arrays reveal multifaceted changes in gene expression during grape berry (Vitis vinifera L.) development

Isogene specific oligo arrays reveal multifaceted changes in gene expression during grape berry (Vitis vinifera L.) development

Molecular linkage maps of Vitis vinifera L. and Vitis riparia Mchx

Generation and Analysis of ESTs in <i>V. amurensis</i> Grapevine Pericarp at Veraison

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