Generation of functional human pancreatic organoids by transplants of embryonic stem cell derivatives in a 3D‐printed tissue trapper

Soltanian, Anahita; Ghezelayagh, Zahra; Mazidi, Zahra; Halvaei, Mana; Mardpour, Soura; Ashtiani, Mohammad Kazemi; Hajizadeh‐Saffar, Ensiyeh; Tahamtani, Yaser; Baharvand, Hossein

doi:10.1002/jcp.27644

Cited by 33 publications

(38 citation statements)

References 57 publications

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“…Interestingly, both hPOs generated fetal pancreatic‐like structures suggesting that the perinatal CF phenotype was not a result of altered pancreatic development (Hohwieler et al ., 2017). In another study, organoids were engineered via co‐culture of three separate cell types (Soltanian et al ., 2018). Both hPSC‐derived endothelial cells and hPSC‐derived MSCs were established and co‐cultured with hPSC‐derived pancreatic progenitors on growth factor‐reduced MG to generate hPOs.…”

Section: Imaging Organoids and In Vivo Transplantationmentioning

confidence: 99%

The science and engineering of stem cell‐derived organoids‐examples from hepatic, biliary, and pancreatic tissues

2020

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The field of organoid engineering promises to revolutionize medicine with wide‐ranging applications of scientific, engineering, and clinical interest, including precision and personalized medicine, gene editing, drug development, disease modelling, cellular therapy, and human development. Organoids are a three‐dimensional (3D) miniature representation of a target organ, are initiated with stem/progenitor cells, and are extremely promising tools with which to model organ function. The biological basis for organoids is that they foster stem cell self‐renewal, differentiation, and self‐organization, recapitulating 3D tissue structure or function better than two‐dimensional (2D) systems. In this review, we first discuss the importance of epithelial organs and the general properties of epithelial cells to provide a context and rationale for organoids of the liver, pancreas, and gall bladder. Next, we develop a general framework to understand self‐organization, tissue hierarchy, and organoid cultivation. For each of these areas, we provide a historical context, and review a wide range of both biological and mathematical perspectives that enhance understanding of organoids. Next, we review existing techniques and progress in hepatobiliary and pancreatic organoid engineering. To do this, we review organoids from primary tissues, cell lines, and stem cells, and introduce engineering studies when applicable. We discuss non‐invasive assessment of organoids, which can reveal the underlying biological mechanisms and enable improved assays for growth, metabolism, and function. Applications of organoids in cell therapy are also discussed. Taken together, we establish a broad scientific foundation for organoids and provide an in‐depth review of hepatic, biliary and pancreatic organoids.

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Section: Imaging Organoids and In Vivo Transplantationmentioning

confidence: 99%

The science and engineering of stem cell‐derived organoids‐examples from hepatic, biliary, and pancreatic tissues

2020

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“…31,53,93,102,106,195–197 Furthermore, it was shown that successful differentiation into early ISC is possible in vitro; maturation, however, requires in vivo conditions. 31,101,196–200 There has been extensive research on the differentiation of insulin-secreting beta cells from human pluri- and multipotent stem cells (n = 47 studies), such as hiPSC, 53,106,107,201,202 hESC, 93,195,197–199,203–206 and MSC. 31,100–102,207–210 Again, these approaches are based on mimicking the processes of natural pancreas embryology by stepwise reproduction with the addition of growth factors and other molecules.…”

Section: Resultsmentioning

confidence: 99%

“…Scaffold size, mechanical stability, and diffusion distances affect the choice further. Among the in vivo studies included (n = 91) the ISC/scaffold construct was transplanted into subcutaneous locations (n = 31), 27,51,69,92–94,112,115,120,133,135,145,147,156,157,171,212–225 the peritoneal cavity (n = 19), 18,26,56,63,65–68,101,134,135,173,195,226–232 an omental pouch (n = 14), 57,59,81,118,119,163,174,225,233–238 the renal capsule (n = 18), 54,…”

Section: Resultsmentioning

confidence: 99%

The emerging field of pancreatic tissue engineering: A systematic review and evidence map of scaffold materials and scaffolding techniques for insulin-secreting cells

et al. 2019

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A bioartificial endocrine pancreas is proposed as a future alternative to current treatment options. Patients with insulin-secretion deficiency might benefit. This is the first systematic review that provides an overview of scaffold materials and techniques for insulin-secreting cells or cells to be differentiated into insulin-secreting cells. An electronic literature survey was conducted in PubMed/MEDLINE and Web of Science, limited to the past 10 years. A total of 197 articles investigating 60 different materials met the inclusion criteria. The extracted data on materials, cell types, study design, and transplantation sites were plotted into two evidence gap maps. Integral parts of the tissue engineering network such as fabrication technique, extracellular matrix, vascularization, immunoprotection, suitable transplantation sites, and the use of stem cells are highlighted. This systematic review provides an evidence-based structure for future studies. Accumulating evidence shows that scaffold-based tissue engineering can enhance the viability and function or differentiation of insulin-secreting cells both in vitro and in vivo.

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“…Interestingly, these hPO integrated into the donor tissue and maintained their phenotypes (Hohwieler, Illing et al 2017). In another study, organoids were engineered via co-culture of three separate cell types (Soltanian, Ghezelayagh et al 2018). Both hPSCderived endothelial cells (EC) and hPSC-derived mesenchymal stem cells (MSC) were established and cocultured with hPSC-derived pancreatic progenitors on growth factor reduced MG to generated hPO.…”

Section: Hepatobiliary and Pancreatic Organoid Transplantationmentioning

confidence: 99%

The Science and Engineering of Stem Cell–Derived Organoids-Examples from Hepatic, Biliary, and Pancreatic Tissues

Ogoke

Maloy

Parashurama

2020

Preprint

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Organoid engineering promises to revolutionize medicine with wide ranging applications of scientific, engineering, and clinical interest, including precision and personalized medicine, gene editing, drug development, disease modeling, cellular therapy, and a basic understanding of human development. Organoids are a three-dimensional (3D), miniature, caricature of a target organ, are initiated with stem/progenitor cells, and are extremely promising tools to model organ function. The biological basis for organoids is that they foster stem cell-self renewal, differentiation, and self-organization, recapitulating tissue structure or function better than 2D systems. In this review, we first discuss the importance of epithelial organs and the general properties of epithelial cells to provide context for the liver, pancreas, and gall bladder and rationale for organoid cultures. Next, we develop a general framework to understand self-organization, tissue hierarchy, and organoid cultivation. For each of these areas, we provide historical context, and review both a wide range of biological and/or biophysical/mathematic perspectives that enhances understanding of organoids. Next, we review existing techniques and progress in hepatobiliary and pancreatic organoid engineering. To do this, we review organoids from both primary tissues, cell lines, and stem cells, and introduce engineering studies when applicable. Noninvasive assessment of 1 organoids can reveal underlying biology and enable improved assays for growth, metabolism, and function. Applications of organoid for cell therapy are also discussed. Taken together, we establish a broad strong scientific foundation for organoids and provide an in-depth review of hepatic, biliary and pancreatic organoids.

show abstract

Generation of functional human pancreatic organoids by transplants of embryonic stem cell derivatives in a 3D‐printed tissue trapper

Cited by 33 publications

References 57 publications

The science and engineering of stem cell‐derived organoids‐examples from hepatic, biliary, and pancreatic tissues

The science and engineering of stem cell‐derived organoids‐examples from hepatic, biliary, and pancreatic tissues

The emerging field of pancreatic tissue engineering: A systematic review and evidence map of scaffold materials and scaffolding techniques for insulin-secreting cells

The Science and Engineering of Stem Cell–Derived Organoids-Examples from Hepatic, Biliary, and Pancreatic Tissues

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