Generation of genome-edited chicken and duck lines by adenovirus-mediated in vivo genome editing

Lee, Joonbum; Kim, Dong Hwan; Karolak, Madeline C.; Shin, Sangsu; Lee, Kichoon

doi:10.1073/pnas.2214344119

Cited by 13 publications

(14 citation statements)

References 16 publications

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“…The germline transmission efficiency varies from 4% to 45%; however, the frequency is comparatively higher than those of other retroviral systems. Recently, adenovirus-mediated CRISPR/Cas9 genome editing in chicken blastoderm PGCs produced genome-edited offspring with 11% germline transmission efficiency [ 35 ].…”

Section: Historical Overview Of Genome Modification Strategies For Av...mentioning

confidence: 99%

“…In addition, an adenovirus containing CRISPR/Cas9 targeting for melanophilin ( MLPH ) gene was injected into the quail blastoderm to produce transgenic quail with 45% germline chimera production efficiency and 2.4% to 10% germline transmission efficiency [ 42 ]. Similarly, adenovirus containing CRISPR/Cas9 was injected into duck blastoderms, producing genome-edited progenies with 2% (duck) germline efficiency [ 35 ]. Despite these successful cases, viral systems are difficult to regard as an effective method owing to the variation in transgenic production efficiency and the limited precision of gene editing.…”

Section: Historical Overview Of Genome Modification Strategies For Av...mentioning

confidence: 99%

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Strategies for the Generation of Gene Modified Avian Models: Advancement in Avian Germline Transmission, Genome Editing, and Applications

Kim¹,

Woo

Han

2023

Genes

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Avian models are valuable for studies of development and reproduction and have important implications for food production. Rapid advances in genome-editing technologies have enabled the establishment of avian species as unique agricultural, industrial, disease-resistant, and pharmaceutical models. The direct introduction of genome-editing tools, such as the clustered regularly interspaced short palindromic repeats (CRISPR) system, into early embryos has been achieved in various animal taxa. However, in birds, the introduction of the CRISPR system into primordial germ cells (PGCs), a germline-competent stem cell, is considered a much more reliable approach for the development of genome-edited models. After genome editing, PGCs are transplanted into the embryo to establish germline chimera, which are crossed to produce genome-edited birds. In addition, various methods, including delivery by liposomal and viral vectors, have been employed for gene editing in vivo. Genome-edited birds have wide applications in bio-pharmaceutical production and as models for disease resistance and biological research. In conclusion, the application of the CRISPR system to avian PGCs is an efficient approach for the production of genome-edited birds and transgenic avian models.

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Section: Historical Overview Of Genome Modification Strategies For Av...mentioning

confidence: 99%

Section: Historical Overview Of Genome Modification Strategies For Av...mentioning

confidence: 99%

Strategies for the Generation of Gene Modified Avian Models: Advancement in Avian Germline Transmission, Genome Editing, and Applications

Kim¹,

Woo

Han

2023

Genes

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“…The effects of the adenoviral transduction of the CRISPR/Cas9 system into local tissue were also demonstrated in chick leg muscle [ 46 ]. More recently, genome-edited ducklings were successfully produced by an adenoviral infection into blastoderms, indicating the efficacy of genome editing technology with adenoviruses in poultry and water birds other than chickens and quails [ 47 ]. Previous studies also confirmed the transduction potential of adenovirus type 5 in chicken, quail, and turkey cells [ 48 , 49 ].…”

Section: Current Approaches For Avian Genome Editing Based On the Cri...mentioning

confidence: 99%

Current Approaches to and the Application of Intracytoplasmic Sperm Injection (ICSI) for Avian Genome Editing

Mizushima

Sasanami

Ono

et al. 2023

Genes

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Poultry are one of the most valuable resources for human society. They are also recognized as a powerful experimental animal for basic research on embryogenesis. Demands for the supply of low-allergen eggs and bioreactors have increased with the development of programmable genome editing technology. The CRISPR/Cas9 system has recently been used to produce transgenic animals and various animals in the agricultural industry and has also been successfully adopted for the modification of chicken and quail genomes. In this review, we describe the successful establishment of genome-edited lines combined with germline chimera production systems mediated by primordial germ cells and by viral infection in poultry. The avian intracytoplasmic sperm injection (ICSI) system that we previously established and recent advances in ICSI for genome editing are also summarized.

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“…For instance, virus isolation and culture, which serve as the gold standard for detecting virus infection, involve a complex operation and take 1−2 weeks to confirm culture results. 10 PCR and ELISA can identify pathogens within a few hours with a detection time of several hours. Nevertheless, these techniques are labor-intensive, require professional operators, and expensive testing instruments, making them unsuitable for point-of-care-testing (POCT).…”

Section: ■ Introductionmentioning

confidence: 99%

“…However, these methods often have their limitations. For instance, virus isolation and culture, which serve as the gold standard for detecting virus infection, involve a complex operation and take 1–2 weeks to confirm culture results . PCR and ELISA can identify pathogens within a few hours with a detection time of several hours.…”

Section: Introductionmentioning

confidence: 99%

A Magnetic-Optical Triple-Mode Lateral Flow Immunoassay for Sensitive and Rapid Detection of Respiratory Adenovirus

Wang,

Li,

Liu

et al. 2024

Anal. Chem.

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Human respiratory adenovirus (ADV) is a highly infectious respiratory virus with potential for pandemics. There are currently no specific drugs to treat ADV worldwide, so early rapid detection of ADV infection is essential. In this study, we developed an innovative magnetic-optical triple-mode lateral flow immunoassay (LFIA) using magnetic quantum dots as immunomarkers. This novel approach addresses the need for rapid and accurate ADV detection, allowing for multimodal quantitative/semiquantitative analysis of magnetic, fluorescent, and visible signals within a mere 15 min. The lower limit of detection (LOD) for magnetic, fluorescent, and visual signals was determined to be 5.6 × 10 3 , 1.2 × 10 3 , and 1.95 × 10 4 copies/mL, respectively. The detection range for ADV using this approach was 1.2 × 10 3 −5 × 10 7 copies/mL. Additionally, semiquantitative analysis, which is user-friendly and does not necessitate specialized equipment, was successfully implemented. Notably, seven respiratory viruses showed no crossreactivity with the generated LFIA test strips. The intrabatch repeatability exhibited a coefficient of variation (CV) of less than 5%, while the interbatch repeatability had a CV of less than 15%. Furthermore, recovery values ranged from 95% to 106.8% for samples analyzed concurrently with dual signals at the same spiking concentration. The assay developed in this study boasts a wide detection range and exceptional sensitivity and specificity. This technique is exceptionally well-suited for on-site rapid detection, with the potential for personal self-testing and early ADV infection diagnosis. Its versatility extends to a broad array of application scenarios.

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Generation of genome-edited chicken and duck lines by adenovirus-mediated in vivo genome editing

Cited by 13 publications

References 16 publications

Strategies for the Generation of Gene Modified Avian Models: Advancement in Avian Germline Transmission, Genome Editing, and Applications

Strategies for the Generation of Gene Modified Avian Models: Advancement in Avian Germline Transmission, Genome Editing, and Applications

Current Approaches to and the Application of Intracytoplasmic Sperm Injection (ICSI) for Avian Genome Editing

A Magnetic-Optical Triple-Mode Lateral Flow Immunoassay for Sensitive and Rapid Detection of Respiratory Adenovirus

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