Generation of handmade cloned embryos from adipose tissue derived mesenchymal stem cells in goat

Malik, Hruda Nanda; Dubey, Amit; Singhal, Dinesh Kumar; Saugandhika, Shrabani; Mohapatra, Sushil K.; Malakar, Dhruba

doi:10.1016/j.smallrumres.2014.09.004

Cited by 6 publications

(3 citation statements)

References 44 publications

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“…So far, several types of cells including neural stem cells, bone marrow‐derived cells, and ADSCs have been used for in vitro and in vivo neural tissue engineering and cell therapy evaluations . But the implication of neural stem cells from the ependymal layer in CNS has been very challenging and limited due to invasive surgeries required to access these cells located in deep areas in the brain or spinal cord.…”

Section: Resultsmentioning

confidence: 99%

A ternary nanofibrous scaffold potential for central nerve system tissue engineering

Saadatkish

Khorasani

Morshed

et al. 2018

J Biomedical Materials Res

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In the present research, a ternary polycaprolactone (PCL)/gelatin/fibrinogen nanofibrous scaffold for tissue engineering application was developed. Through this combination, PCL improved the scaffold mechanical properties; meanwhile, gelatin and fibrinogen provided more hydrophilicity and cell proliferation. Three types of nanofibrous scaffolds containing different fibrinogen contents were prepared and characterized. Morphological study of the nanofibers showed that the prepared nanofibers were smooth, uniform without any formation of beads with a significant reduction in nanofiber diameter after incorporation of fibrinogen. The chemical characterization of the scaffolds confirmed that no chemical reaction occurred between the scaffold components. The tensile test results of the scaffolds showed that increasing in fibrinogen content led to a decrease in mechanical properties. Furthermore, adipose-derived stem cells were employed to evaluate cell-scaffold interaction. Cell culture results indicated that higher cell proliferation occurred for the higher amount of fibrinogen. Statistical analysis was also carried out to evaluate the significant difference for the obtained results of water droplet contact angle and cell culture. Therefore, the results confirmed that PCL/gel/fibrinogen scaffold has a good potential for tissue engineering applications including central nerve system tissue engineering. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A:2394-2401, 2018.

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Section: Resultsmentioning

confidence: 99%

A ternary nanofibrous scaffold potential for central nerve system tissue engineering

Saadatkish

Khorasani

Morshed

et al. 2018

J Biomedical Materials Res

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“…Initial attempts to use zone-free procedures, especially in embryonic cell nuclear transfer, have led to the first successful report to produce cloned cattle, using a somatic cell as nucleus donor (Vajta et al, 2001). In sheep and goats, initial publications have reported the application of this technique for successful embryo development (Peura and Vajta, 2003; Akshey et al, 2008), followed by a number of publications reporting the application of this technique to produce cloned (Malik et al, 2014; Khan et al, 2018), transgenically cloned (Lagutina et al, 2007; Pereira et al, 2013), or interspecies cloned embryos (Selokar et al, 2011; Yu et al, 2011). An interesting example of the application of this technique in sheep is the generation of transgenic cloned lambs carrying a modified nematode mfat-1 gene to enrich muscles and other organs and tissues with omega-3 fatty acids (Zhang et al, 2013).…”

Section: Overview Of Genetic Modification and Relevant Biotechnologicmentioning

confidence: 99%

Sheep and Goat Genome Engineering: From Random Transgenesis to the CRISPR Era

et al. 2019

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Sheep and goats are valuable livestock species that have been raised for their production of meat, milk, fiber, and other by-products. Due to their suitable size, short gestation period, and abundant secretion of milk, sheep and goats have become important model animals in agricultural, pharmaceutical, and biomedical research. Genome engineering has been widely applied to sheep and goat research. Pronuclear injection and somatic cell nuclear transfer represent the two primary procedures for the generation of genetically modified sheep and goats. Further assisted tools have emerged to enhance the efficiency of genetic modification and to simplify the generation of genetically modified founders. These tools include sperm-mediated gene transfer, viral vectors, RNA interference, recombinases, transposons, and endonucleases. Of these tools, the four classes of site-specific endonucleases (meganucleases, ZFNs, TALENs, and CRISPRs) have attracted wide attention due to their DNA double-strand break-inducing role, which enable desired DNA modifications based on the stimulation of native cellular DNA repair mechanisms. Currently, CRISPR systems dominate the field of genome editing. Gene-edited sheep and goats, generated using these tools, provide valuable models for investigations on gene functions, improving animal breeding, producing pharmaceuticals in milk, improving animal disease resistance, recapitulating human diseases, and providing hosts for the growth of human organs. In addition, more promising derivative tools of CRISPR systems have emerged such as base editors which enable the induction of single-base alterations without any requirements for homology-directed repair or DNA donor. These precise editors are helpful for revealing desirable phenotypes and correcting genetic diseases controlled by single bases. This review highlights the advances of genome engineering in sheep and goats over the past four decades with particular emphasis on the application of CRISPR/Cas9 systems.

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“…El carioplasto es la célula somática que aporta el total del material genético necesario para el desarrollo del individuo clonado. Entre los tipos celulares utilizados como donadores de núcleo se han usado: células embrionarias (Peura et al, 1998), células del cúmulo (Vajta et al, 2001(Vajta et al, y 2003, fibroblastos fetales (Regio et al, 2001;Vajta et al, 2003;Meena et al, 2006;Akshey et al, 2008;Zhang et al, 2009), fibroblastos de individuos adultos (Beyhan et al, 2002;Elsheikh et al, 2006;Shah et al, 2008;Ribeiro et al, 2009;Dutta et al, 2011;Zhang et al, 2013;Khan et al, 2014;Saini et al, 2015;), tejido adiposo (Malik et al, 2014) y células troncales embrionales (Mohapatra et al, 2015). Aunque son varios los tipos celulares que pueden ser utilizados como donadores de núcleo, Saini et al (2015) indican que los fibroblastos son las células más adecuadas para lograr óptimos resultados en la TNCS, debido a la facilidad con que pueden ser reprogramadas.…”

Section: Células Donadoras De Núcleo (Carioplastos)unclassified

Obtención de embriones de ovino por transferencia nuclear a partir de Fibroblastos de piel y ovocitos maduros in vitro con resveratrol

Ibarra

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A la Dra. María del Carmen Navarro por la dirección de la presente tesis y por brindarme el apoyo y la libertad para desarrollar esta investigación.Al Dr. Demetrio Ambriz por su apoyo incondicional durante toda la maestría, el cual fue fundamental para la culminación de esta tesis. Pero en particular por la amistad y la confianza brindada durante el tiempo que llevamos de conocernos.Al Dr. Raymundo Rangel por su colaboración como asesor y revisor de la presente tesis.A la Dra. Edith Arenas por compartir conmigo su conocimiento y por las palabras de apoyo que me ha brindado.

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Generation of handmade cloned embryos from adipose tissue derived mesenchymal stem cells in goat

Cited by 6 publications

References 44 publications

A ternary nanofibrous scaffold potential for central nerve system tissue engineering

A ternary nanofibrous scaffold potential for central nerve system tissue engineering

Sheep and Goat Genome Engineering: From Random Transgenesis to the CRISPR Era

Obtención de embriones de ovino por transferencia nuclear a partir de Fibroblastos de piel y ovocitos maduros in vitro con resveratrol

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