Generation of High-Value Genomic Resource in Rice: A “Subgenomic Library” of Low-Light Tolerant Rice Cultivar Swarnaprabha

Abstract

Rice is the major staple food crop for more than 50% of the world’s total population, and its production is of immense importance for global food security. As a photophilic plant, its yield is governed by the quality and duration of light. Like all photosynthesizing plants, rice perceives the changes in the intensity of environmental light using phytochromes as photoreceptors, and it initiates a morphological response that is termed as the shade-avoidance response (SAR). Phytochromes (PHYs) are the most important photoreceptor family, and they are primarily responsible for the absorption of the red (R) and far-red (FR) spectra of light. In our endeavor, we identified the morphological differences between two contrasting cultivars of rice: IR-64 (low-light susceptible) and Swarnaprabha (low-light tolerant), and we observed the phenological differences in their growth in response to the reduced light conditions. In order to create genomic resources for low-light tolerant rice, we constructed a subgenomic library of Swarnaprabha that expedited our efforts to isolate light-responsive photoreceptors. The titer of the library was found to be 3.22 × 105 cfu/mL, and the constructed library comprised clones of 4–9 kb in length. The library was found to be highly efficient as per the number of recombinant clones. The subgenomic library will serve as a genomic resource for the Gramineae community to isolate photoreceptors and other genes from rice.

Drought and Oxidative Stress in Flax (Linum usitatissimum L.) Entails Harnessing Non-Canonical Reference Gene for Precise Quantification of qRT-PCR Gene Expression

“…Over the last decade, the advent of new genomic tools has hastened research with the generation of enormous biological data [ 46 , 47 , 48 , 49 , 50 , 51 ] with high accuracy and efficiency [ 52 , 53 , 54 , 55 , 56 ]. These techniques have also enabled visualization of the impact of experiments/reactions in real-time.…”

Phylogenomic Analysis of micro-RNA Involved in Juvenile to Flowering-Stage Transition in Photophilic Rice and Its Sister Species

“…Grain production in field crops has doubled in the last decades due to the "Green revolution" [31], along with advances in functional [32][33][34][35] and structural [36][37][38][39][40][41][42] genomics. Further, marker-assisted back-cross breeding (MABB) imparted biotic [43][44][45][46] and abiotic [47][48][49][50] stress tolerance, ultimately leading to higher grain yield.…”

Effects of Low Light after Heading on the Yield of Direct Seeding Rice and Its Physiological Response Mechanism

“…Light is an important factor in rice production. Changes in light intensity and quality directly lead to changes in the physiological indicators, yield, and quality of rice [ 4 , 5 ]. In recent years, ‘shading’ caused by air pollution and clouds has become an important limiting factor for rice production in China, India, and other countries [ 6 ].…”