Generation of human induced pluripotent stem cell line UGENTi001-A from a patient with Marfan syndrome carrying a heterozygous c.7754 T > C variant in FBN1 and the isogenic control UGENT001-A-1 using CRISPR/Cas9 editing

Photoporation-mediated spatial intracellular delivery of stem cell-derived cardiomyocytes

“…To validate the described method, iPSCs (described in [ 12 ]) were differentiated to cardiomyocytes by addition of small molecules (Supplement 1). After the temporal modulation of the Wnt pathway, the differentiated cardiomyocytes were passaged to coverslips, after which the cardiomyocyte purity and phenotype was analyzed on day 30 after start of differentiation.…”

Association Analyses between Single Nucleotide Polymorphisms in ZFAT, FBN1, FAM184B Genes and Litter Size of Xinggao Mutton Sheep

“…The result of the protein evolutionary tree displayed that two model animals (Capra hircus and Bos taurus) had the closest protein evolutionary relationship with sheep. In addition, Homo sapiens would also been involved in the protein interaction network analysis because there are many similarities in the reproduction mechanism between humans and sheep (bicorned uterus and similar ovulation rate) and in-depth function studies have been carried out on FBN1 protein in Homo sapiens [20,21]. Protein interaction network analysis for Capra hircus, Homo sapiens and Bos taurus demonstrated the 10 proteins most closely interacting with FBN1 (Figure 3B-D), and, among them, the five proteins (ELN, LUM, MFAP5, COL1A2, COL5A2) were associated with the proliferation of ovarian granulosa cells and cell markers for theca interna and granulosa cells [22][23][24].…”