Generation of Indole Alkaloids in the Human‐Pathogenic Fungus <i>Exophiala dermatitidis</i>

Kindler, Bernhard L. J.; Krämer, Hans‐Joachim; Nies, Silke; Gradicsky, Philipp; Haase, G.; Mayser, Peter; Spiteller, Michael; Spiteller, Peter

doi:10.1002/ejoc.200901311

Cited by 15 publications

(5 citation statements)

References 23 publications

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“…However, these peaks may be established to have minor significance in comparison to the peak intensity ratio obtained to the aromatic and aliphatic signals in the 13 C spectrum. With an overall comparison of the FTIR spectrum with the 13 C NMR study, the presence of hydroxyl-naphthalene moiety can be emphasized to be present in its tautomeric form as reported in previous studies [64] , [65] , [66] , [67] . Further, as the extracted compound is confirmed to be chemically similar to the melanin obtained from several different sources using complementary techniques, melanin constituted by the hydroxy-naphthalene moiety is reported for melanin extracted from A. fumigatus AFGRD105.…”

Section: Discussionmentioning

confidence: 67%

Genetic validation and spectroscopic detailing of DHN-melanin extracted from an environmental fungus

Raman

Ramasamy

2017

Biochemistry and Biophysics Reports

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Accurate characterization of melanin using analytical methodologies has proved to be difficult due to its heterogeneity, insolubility in wide pH and broad range of solvents. The present study was undertaken to characterize melanin extracted from an environmental Aspergillus fumigatus AFGRD105 by studying its genes, chemical properties and spectral data. A gene based approach to confirm the type of melanin carried out indicated the extracted melanin to be of the dihydroxynaphthalene type. On comparison with synthetic melanin, UV–Vis and IR spectra of the extracted melanin revealed characteristic peaks that can be further used for confirmation of DHN-melanin extracted from any source. Solid state 13C NMR spectroscopy established the presence of the hydroxyl-naphthalene moiety and validated the results obtained by genetic analysis. The correct assignment of the observed spectral frequency characteristic of functional groups can be further adapted in future works that deal with binding capacities and biomolecule systems involving melanin.

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Section: Discussionmentioning

confidence: 67%

Genetic validation and spectroscopic detailing of DHN-melanin extracted from an environmental fungus

Raman

Ramasamy

2017

Biochemistry and Biophysics Reports

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“…In addition, adherence and biofilm formation have been shown to contribute to virulence. Further virulence attributes of E. dermatitidis are hydrophobicity [96], urease, catalase, proteinase and DNase production [97], chitin synthase [98][99][100], secondary metabolite production [96,101] and the assimilation of aromatic (alkylbenzene) hydrocarbons [53,102,103]. Latter contributes to neurotropism characteristics of members of Herpotrichiellaceae [53].…”

Section: Microbiology and Characteristicsmentioning

confidence: 99%

Exophiala dermatitidis: Key issues of an opportunistic fungal pathogen

et al. 2019

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The black yeast Exophiala dermatitidis is an opportunistic pathogen, causing phaeohyphomycosis in immunosuppressed patients, chromoblastomycosis and fatal infections of the central nervous system in otherwise healthy Asian patients. In addition, it is also regularly isolated from respiratory samples from cystic fibrosis patients, with rates varying between 1% and 19%.Melanin, as part of the cell wall of black yeasts, is one major factor known contributing to the pathogenicity of E. dermatitidis and increased resistance against host defense and anti-infective therapeutics. Further virulence factors, e.g. the capability to adhere to surfaces and to form biofilm were reported. A better understanding of the pathogenicity of E. dermatitidis is essential for the development of novel preventive and therapeutic strategies. In this review, the current knowledge of E. dermatitidis prevalence, clinical importance, diagnosis, microbiological characteristics, virulence attributes, susceptibility, and resistances as well as therapeutically strategies are discussed.

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“…3). 15 Seven new indole alkaloids, bruceollines H (16), I (17), J (18), K (19), L (20), M (21), and N (22) were isolated from the ethanol extract of the stems of Brucea mollis Wall., distributed in southern China. The cytotoxic activities of all compounds were tested in vitro against ve human cell lines using the MTT method (Fig.…”

Section: Non-tryptaminesmentioning

confidence: 99%

“…5). 18 Two new simple indole alkaloids, verticillanes A (25) and B (26), were isolated from Borreria verticillata (L.) G. MEY. known as 'Cordão-de-Frade' (Fig.…”

Section: Non-tryptaminesmentioning

confidence: 99%