Generation of Interleukin 1 at Inflammatory Sites: Kinetics and Producing Cells

Goto, F; Mori, Shunsuke; Goto, Kumiko; Ohkawara, Susumu; Yoshinaga, Masaru

doi:10.5551/jat1973.16.8_1097

Cited by 10 publications

(15 citation statements)

References 3 publications

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“…Possible factor candidates are cytokines and glucocorticoids because they are secreted fol lowing tissue injury and play important roles in the inflammatory process (24)(25)(26)(27) and also because regulatory communication exists be tween them (28). A number of cytokines, in cluding interleukin-1, interleukin-6 and tumor necrosis factor, also affect the production of acute phase proteins in the liver (25,29,30) and lipid metabolism (31).…”

Section: Discussionmentioning

confidence: 99%

A Uniform Alteration in Serum Lipid Metabolism Occurring during Inflammation in Mice.

et al. 1992

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ABSTRACT-The present study was designed to delineate changes in serum lipid levels following various kinds of tissue injury or inflammation such as contact sensitiv ity to picryl chloride, thermal burn, carrageenin-induced edema, the administration of turpentine oil, Freund's complete adjuvant (FCA), killed Bordetella pertussis (BP) or lipopolysaccharide (LPS). A uniform change in the serum lipid metabolism ,was observed in mice that received these inflammatory stimuli; that is, increases in total cholesterol, free cholesterol and phospholipid levels, a decrease in the ester ratio and a decline in lecithin: cholesterol acyltransferase activity as well as a decrease in albu min levels, which is an index of the acute-phase response. However, serum triglyceride levels were increased by treatment with the bacterial stimuli (FCA, BP and LPS) but decreased by treatment with the other stimuli. The serum free cholesterol and phos pholipid levels were significantly correlated with the intensity of contact sensitivity, which was modified by treatment with cyclophosphamide. Indomethacin or dex amethasone suppressed carrageenin-induced edema and inhibited some of the altera tions in lipid metabolism that developed during inflammation because each affected a part of the lipid metabolism. These findings suggest that, like the appearance of acute-phase proteins, the uniform change in serum lipid metabolism may be another sensitive index of the acute inflammatory response.

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Section: Discussionmentioning

confidence: 99%

A Uniform Alteration in Serum Lipid Metabolism Occurring during Inflammation in Mice.

et al. 1992

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“…Recombinant rabbit IL-I p, TNFa and IL-1 receptor antagonist (IL-IRa) were purified, as described elsewhere [7,21,22]. Neutralizing anti-rabbit TNFa mAb (anti-TNFa mAb) was kindly provided by Dr. H. Nariuchi (University of Tokyo, Japan) [8].…”

Section: Reagent5mentioning

confidence: 99%

Expression of monocyte chemoattractant protein‐1 in primary cultures of rabbit intervertebral disc cells

Yoshida

Nakamura

Kikuchi

et al. 2002

Journal Orthopaedic Research

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Macrophages are considered essential for herniated disc resorption, and chemokines may play a role in their recruitment. Here we demonstrate that intervertebral disc cells are capable of producing monocyte chemoattractant protein-1 (MCP-I), a CC chemokine that is chemotactic for macrophages. Nucleus pulposus cells and anulus fibrosus cells were harvested from intervertebral discs of healthy rabbits, and the cells were stimulated with either interleukin (IL)-lp or tumor necrosis factor (TNF)c(. Reverse transcriptase polymerase chain reaction demonstrated that IL-1 p and TNFa induced mRNA expression for MCP-I in nucleus pulposus and anulus fibrosus cells. Protein concentrations of MCP-1 in the culture supernatants were quantitated by fluoroimmunoassay, which showed that nucleus pulposus and anulus fibrosus cells dose-and time-dependently produced MCP-1 after IL-1 band TNFcc-stimulation, an event that was completely abrogated by 1L-1 receptor antagonist and anti-TNFct monoclonal antibody, respectively. Nucleus pulposiis cells produced significantly higher levels of MCP-1 than did anulus fibrosus cells. Immunohistochemically, the intensity of MCP-1 positive cells in nucleus pulposus cells was stronger than that in anulus fibrosus cells. Altogether, our data clearly demonstrated the production of MCP-1 in intervertebral disc cells, suggesting the possible involvement of disc cells in an early stage of macrophage infiltration.

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“…Rabbit serum albumin was purchased from Organ Technique Co. (Durham, NC, U.S.A.). Recombinant rabbit (rr)TNFα, rrIL-1β and rrIL-1Ra were purified from extracts of E. coli containing the mature form of each cytokine cDNA, as described elsewhere (Goto et al, 1989 ;Matsukawa et al, 1992Matsukawa et al, , 1997. Neutralizing antirabbit TNFα mAb (IgG1) was purified from ascites containing hybridoma cells (Haranaka et al, 1985).…”

Section: Agentsmentioning

confidence: 99%

Involvement of TNF α, IL-1β and IL-1 Receptor Antagonist in LPS-induced Rabbit Uveitis

Matsukawa

Ohkawara

et al. 1998

Experimental Eye Research

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Generation of Interleukin 1 at Inflammatory Sites: Kinetics and Producing Cells

Cited by 10 publications

References 3 publications

A Uniform Alteration in Serum Lipid Metabolism Occurring during Inflammation in Mice.

A Uniform Alteration in Serum Lipid Metabolism Occurring during Inflammation in Mice.

Expression of monocyte chemoattractant protein‐1 in primary cultures of rabbit intervertebral disc cells

Involvement of TNF α, IL-1β and IL-1 Receptor Antagonist in LPS-induced Rabbit Uveitis

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