Generation of Live Young from Xenografted Mouse Ovaries

Snow, Melanie Jennifer; Cox, Shae-Lee; Jenkin, Graham; Trounson, Alan; Shaw, Jillian M

doi:10.1126/science.1073693

Cited by 83 publications

(56 citation statements)

References 7 publications

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“…Oocytes collected from ovarian grafts, irrespective of the position of the graft site have significantly lower two-cell embryo cleavage rates after IVF. This confirms previous studies where low numbers of embryos produced from orthotopic (Aubard et al 1999, Oktay & Yih 2002 and heterotopic grafts were reported (Aubard et al 1999, Snow et al 2002, Lee et al 2004, Oktay et al 2004, Waterhouse et al 2004. Collectively this indicates that further work is required to determine why oocytes that develop in ovarian grafts have reduced developmental competence.…”

Section: Discussionsupporting

confidence: 89%

“…Previous studies where exogenous PMSG was administered to stimulate ovarian tissue grafted under the kidney capsule also failed to increase the yield of oocytes from ovarian grafts (Snow et al 2002, Waterhouse et al 2004. Collectively, data from this study and previous studies indicate that developing follicles in ovarian grafts, irrespective of the graft site, do not respond to exogenous gonadotrophins in the same manner as intact ovaries.…”

Section: Discussionsupporting

confidence: 51%

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Graft site and gonadotrophin stimulation influences the number and quality of oocytes from murine ovarian tissue grafts

Yang¹,

Cox²,

Jenkin³

et al. 2006

Reproduction

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Ovarian tissue cryopreservation and subsequent transplantation can restore fertility in cancer patients. This study used a mouse ovarian grafting model to investigate whether the graft site (bursal cavity, the kidney capsule or subcutaneous) influences the number, fertilization rate and developmental potential of oocytes recovered from grafts and whether using a standard gonadotrophin stimulation protocol would increase oocyte yield from the grafts. Mouse ovarian tissue was grafted into four week old mice and collected three weeks later. Graft recipients were treated either with or without exogenous gonadotrophin stimulation prior to graft collection. Grafted ovaries yielded oocytes that were either at the germinal vesicle (GV) stage or mature metaphase II (MII) stage at collection. These GV oocytes were matured before in vitro fertilization (IVF), while the MII oocytes underwent IVF immediately. Oocytes collected from the oviducts of non-grafted superovulated mice of the same age served as controls. Two-cell embryos were transferred to pseudopregnant recipients and recovered at day 15 of gestation or left to go to term. Graft retrieval and the number of oocytes from each graft were lowest from the subcutaneous graft site. The number of two-cell embryos produced was significantly higher for oocytes from the grafts to the bursa as compared with the other sites. All graft sites gave rise to embryos with comparable implantation rates and developmental potential to fetuses and offspring following transfer. However, the oocytes from grafted ovaries had a significantly lower developmental potential when compared with the control group. Stimulation with exogenous gonadotrophins did not significantly increase oocyte yield from grafted ovaries but did enhance oocyte maturation and development. In conclusion, graft site affects the number and quality of oocytes produced from ovarian grafts.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 51%

Graft site and gonadotrophin stimulation influences the number and quality of oocytes from murine ovarian tissue grafts

Yang¹,

Cox²,

Jenkin³

et al. 2006

Reproduction

Self Cite

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“…Even foreign grafts of parenchymal cells such as liver and brain [40][41][42], privileged by low MHC class I expression, necessitate impaired host immunity for survival. For example, hepatocyte xenografts in rabbits and rodents die rapidly without immunosuppression [43][44][45], and the survival of ovarian xenografts in adult rats requires nude hosts [46]. In comparison, little is known about the immunogenicity of differentiation-competent progenitor cells.…”

Section: Discussionmentioning

confidence: 99%

Embryonic Mouse STO Cell–Derived Xenografts Express Hepatocytic Functions in the Livers of Nonimmunosuppressed Adult Rats

et al. 2005

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Cells derived from embryonic mouse STO cell lines differentiate into hepatocytes when transplanted into the livers of nonimmunosuppressed dipeptidylpeptidase IV (DPPIV)-negative F344 rats. Within 1 day after intrasplenic injection, donor cells moved rapidly into the liver and were found in intravascular and perivascular sites; by 1 month, they were intrasinusoidal and also integrated into hepatic plates with approximately 2% efficiency and formed conjoint bile canaliculi. Neither donor cell proliferation nor host inflammatory responses were observed during this time. Detection of intrahepatic mouse COX1 mitochondrial DNA and mouse albumin mRNA in recipient rats indicated survival and differentiation of donor cells for at least 3 months. Mouse COX1 targets were also detected intrahepatically 4-9 weeks after STO cell injection into nonimmunosuppressed wild-type rats. In contrast to STO-transplanted rats, mouse DNA or RNA was not detectable in untreated or mock-transplanted rats or in rats injected with donor cell DNA. In cultured STO donor cells, DPPIV and glucose-6-phosphatase activities were observed in small clusters; in contrast, mouse major histocompatibility complex class I H-2Kq , H-2D q , and H-2L q and class II I-A q markers were undetectable in vitro before or after interferon gamma treatment. Together with H-2K allele typing, which confirmed the Swiss mouse origin of the donor cells, these observations indicate that mouse-derived STO cell lines can differentiate along hepatocytic lineage and engraft into rat liver across major histocompatibility barriers.

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“…Cross-species ovarian grafting (xenografting) is advantageous for multiplication and conservation of domestic or endangered animals, because the ovaries can be collected from deceased animals and the oocytes can potentially be grown in a different species. Snow et al (2002) demonstrated that oocytes that grow within mouse ovarian tissue xenografted to nude rats acquire the ability to generate pups. Ovarian tissues have been prepared from species phylogenetically distant from mice, including humans (Oktay et al 1998, Weissman et al 1999, Kim et al 2002, Gook et al 2003, dogs (Metcalfe et al 2001), monkeys (Candy et al 1995), sheep (Gosden et al 1994), cows (Senbon et al 2003), pigs (Kaneko et al 2003, Kagawa et al 2005, tammar wallaby (Mattiske et al 2002) and common wombats (Cleary et al 2003(Cleary et al , 2004, and xenografted to immunodeficient mice.…”

Section: Introductionmentioning

confidence: 99%

Effects of gonadotrophin treatments on meiotic and developmental competence of oocytes in porcine primordial follicles following xenografting to nude mice

Kaneko¹,

Kikuchi²,

Noguchi³

et al. 2006

Reproduction

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Our objective was to improve the developmental ability of oocytes in porcine primordial follicles xenografted to nude mice, by treating the host mice with gonadotrophins to accelerate follicular growth. Ovarian tissues from 20-day-old piglets, in which most of the follicles were primordial, were transplanted under the kidney capsules of ovariectomized nude mice. Gonadotrophin treatments were commenced around 60 days after vaginal cornification in the mice. Ovarian grafts were obtained 2 or 3 days after treatment with equine chorionic gonadotrophin (eCG-2 and eCG-3 groups), after porcine FSH infusion for 7 or 14 days, or after infusion of porcine FSH for 14 days with a single injection of estradiol antiserum (FSH-7, FSH-14 and FSH-14EA groups, respectively). Gonadotrophin treatments accelerated follicular growth within the xenografts compared with that in control mice given no gonadotrophins, consistent with higher (P < 0.05) circulating inhibin levels in the gonadotrophin-treated mice. In contrast, circulating mouse FSH levels were significantly (P < 0.05) depressed. We recovered large numbers of full-sized oocytes with meiotic competence to the mature stage from the eCG-3, FSH-7, and FSH-14EA, unlike in the control group. Moreover, 56% of matured oocytes with the first polar body (n 5 39) were fertilized in vitro in the FSH-14EA group. After in vitro fertilization and subsequent culture for 7 days, one blastocyst was obtained from each of the eCG-3, FSH-7 and, FSH-14EA groups, whereas no blastocysts appeared in the other groups. Exogenous gonadotrophinsnot mouse FSH -stimulated the growing follicles that had developed from the primordial follicles in the xenografts: the effects were incomplete but improved to some extent the meiotic and developmental abilities of the oocytes.

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Generation of Live Young from Xenografted Mouse Ovaries

Cited by 83 publications

References 7 publications

Graft site and gonadotrophin stimulation influences the number and quality of oocytes from murine ovarian tissue grafts

Graft site and gonadotrophin stimulation influences the number and quality of oocytes from murine ovarian tissue grafts

Embryonic Mouse STO Cell–Derived Xenografts Express Hepatocytic Functions in the Livers of Nonimmunosuppressed Adult Rats

Effects of gonadotrophin treatments on meiotic and developmental competence of oocytes in porcine primordial follicles following xenografting to nude mice

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