2010
DOI: 10.1128/jcm.01691-09
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Generation of Merkel Cell Polyomavirus (MCV)-Like Particles and Their Application to Detection of MCV Antibodies

Abstract: The genome of a new human polyomavirus, known as Merkel cell polyomavirus (MCV), has recently been reported to be integrated within the cellular DNA of Merkel cell carcinoma (MCC), a rare human skin cancer. To investigate MCV seroprevalence in the general population, we expressed three different MCV VP1 in insect cells using recombinant baculoviruses. Viruslike particles (VLPs) were obtained with only one of the three VP1 genes. High-titer antibodies against VP1 VLPs were detected in mice immunized with MCV VL… Show more

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Cited by 112 publications
(139 citation statements)
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“…These data suggest that MCPyV infects a large proportion of the population, where it induces a physiological immune response (28,32,33).…”
Section: Discussionmentioning
confidence: 97%
See 1 more Smart Citation
“…These data suggest that MCPyV infects a large proportion of the population, where it induces a physiological immune response (28,32,33).…”
Section: Discussionmentioning
confidence: 97%
“…27), were detected by ELISA using MCPyV virus-like particles (VLP) and the recombinant LT/ST. VLPs and LT/ST were generated and purified as previously described (27,28). Detection of VP1 and LT/ST antibodies by indirect ELISA was performed as previously described (27,28).…”
Section: Detection Of Serum Igg Antibodies Against Mcpyv Vp1 Proteinmentioning
confidence: 99%
“…Seroprevalence of 50-95% is reported in adults (7,(12)(13)(14)(25)(26)(27)(28)(29). The age-specific seroprevalence of MCPyV (Fig.…”
Section: Serologymentioning
confidence: 99%
“…When expressed in mammalian or insect cells, VP1 proteins (or VP1 + VP2 proteins) self-assemble into 45-55 nm diameter virus-like particles (VLPs) (Fig. 2) that are used in serological assays (7,(12)(13)(14).…”
Section: Mcpyv Virology and Genomic Organizationmentioning
confidence: 99%
“…Purified MWPyV VLPs (100 ng/well in phosphate-buffered saline [PBS]) were used to sensitize microplates (Maxisorp; Nunc) overnight at 4°C. Briefly, sera were diluted 1:100, and peroxidase-conjugated anti-human IgG (Southern Biotech, Clinisciences, Nanterre, France) diluted 1:20,000 was used to detect human IgG binding (14). A histogram of the ELISA optical density (OD) values in 1-to 10-year-old children (data not shown) revealed a bimodal distribution of seroreactivity.…”
mentioning
confidence: 99%