Generation of model tissues with dendritic vascular networks via sacrificial laser-sintered carbohydrate templates

Kinstlinger, Ian S.; Saxton, Sarah; Calderon, Gisele A.; Ruiz, Karen Vasquez; Yalacki, David R.; Deme, Palvasha; Rosenkrantz, Jessica E.; Louis-Rosenberg, Jesse D.; Johansson, Fredrik; Janson, Kevin; Sazer, Daniel W.; Panchavati, Saarang S.; Bissig, Karl‐Dimiter; Stevens, Kelly R.; Miller, Jordan S.

doi:10.1038/s41551-020-0566-1

Cited by 113 publications

(91 citation statements)

References 88 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a sacrificial approach, a template can be printed with predesigned 3D structure through extrusion bioprinting (29)(30)(31) and selective laser sintering (32). The template is then submerged in a cell-embedded supporting hydrogel (31), or the template can be directly extruded into the supporting hydrogel (29,33,34).…”

Section: Bioprinted Microvasculaturementioning

confidence: 99%

“…Subsequently, the template is sacrificed by exploiting its thermal or chemical properties and flushed out of the hydrogel system. For example, Pluronic F127 (30) and gelatin (35) liquidize at 0 and 37 • C, respectively, while carbohydrate (32,36) and salts dissolve in cell culture medium rapidly after immersion. Hollow microchannels are then endothelialized in vitro to allow faster inosculation after implantation (31,37).…”

Section: Bioprinted Microvasculaturementioning

confidence: 99%

See 1 more Smart Citation

Applications of Engineering Techniques in Microvasculature Design

Halawani

Wang

Liu

et al. 2021

Front. Cardiovasc. Med.

View full text Add to dashboard Cite

Achieving successful microcirculation in tissue engineered constructs in vitro and in vivo remains a challenge. Engineered tissue must be vascularized in vitro for successful inosculation post-implantation to allow instantaneous perfusion. To achieve this, most engineering techniques rely on engineering channels or pores for guiding angiogenesis and capillary tube formation. However, the chosen materials should also exhibit properties resembling the native extracellular matrix (ECM) in providing mechanical and molecular cues for endothelial cells. This review addresses techniques that can be used in conjunction with matrix-mimicking materials to further advance microvasculature design. These include electrospinning, micropatterning and bioprinting. Other techniques implemented for vascularizing organoids are also considered for their potential to expand on these approaches.

show abstract

Section: Bioprinted Microvasculaturementioning

confidence: 99%

Section: Bioprinted Microvasculaturementioning

confidence: 99%

Applications of Engineering Techniques in Microvasculature Design

Halawani

Wang

Liu

et al. 2021

Front. Cardiovasc. Med.

View full text Add to dashboard Cite

show abstract

“…Biological materials possess hierarchical vascular networks that mediate heat and mass transport in response to external and internal stimuli, enabling complex living systems to thrive in extreme environments 1 , 2 . The replication of such pervasive vascular networks in engineered tissues and organoids for improving cellular proliferation 3 – 5 , studying fluid flow 6 , and pneumatic actuation 7 has garnered significant interest in therapeutics, prosthetics, and soft robotics 3 – 6 . Vascular and porous synthetic materials are also used for electrical insulation in microelectronics 8 , gas exchange in synthetic devices 9 , 10 , thermal management in heat exchangers and fiber-reinforced composites 11 , 12 , chemical reactions in flow batteries and microreactors 13 – 15 , and material regeneration in self-healing structures 16 , 17 .…”

Section: Introductionmentioning

confidence: 99%

Rapid synchronized fabrication of vascularized thermosets and composites

Garg

Zhang

et al. 2021

Nat Commun

View full text Add to dashboard Cite

Bioinspired vascular networks transport heat and mass in hydrogels, microfluidic devices, self-healing and self-cooling structures, filters, and flow batteries. Lengthy, multistep fabrication processes involving solvents, external heat, and vacuum hinder large-scale application of vascular networks in structural materials. Here, we report the rapid (seconds to minutes), scalable, and synchronized fabrication of vascular thermosets and fiber-reinforced composites under ambient conditions. The exothermic frontal polymerization (FP) of a liquid or gelled resin facilitates coordinated depolymerization of an embedded sacrificial template to create host structures with high-fidelity interconnected microchannels. The chemical energy released during matrix polymerization eliminates the need for a sustained external heat source and greatly reduces external energy consumption for processing. Programming the rate of depolymerization of the sacrificial thermoplastic to match the kinetics of FP has the potential to significantly expedite the fabrication of vascular structures with extended lifetimes, microreactors, and imaging phantoms for understanding capillary flow in biological systems.

show abstract

“…Some efforts have used sacrificial laser-sintered for constructing vascular networks, but this indirect printing method was detrimental to integrated bioprinting using multi-materials. [29] Therefore, we propose a method for bioprinting vasculature structure using elastic hydrogel and cell-laden hydrogel for enhanced mechanical support and biological activity. Cell-laden hydrogels may potentially decrease the damage during bioprinting and solidify in a cell-friendly environment, which may result in optimal cell proliferation and tissue remodeling.…”

Section: Introductionmentioning

confidence: 99%

A novel method for generating 3D constructs with branched vascular networks using multi-materials bioprinting and direct surgical anastomosis

Liu

Wang

Zhang

et al. 2021

Preprint

View full text Add to dashboard Cite

Vessels pervade almost all body tissues, and significantly influence the pathophysiology of human body. Previous attempts to establish multi-scale vascular connection and function in 3D model tissues using bioprinting have had limited success due to the incoordination between cell-laden materials and stability of the perfusion channel. Here, we report a methodology to fabricate centimetre-scale vascularized soft tissue with high viability and accuracy using multi-materials bioprinting involving inks with low viscosity and a customized multistage-temperature-control printer. The tissue formed was perfused with branched vasculature with well-formed 3D capillary network and lumen, which would potentially supply the cellular components with sufficient nutrients in the matrix. Furthermore, the same methodology was applied for generating liver-like tissue with the objective to fabricate and mimic a mature and functional liver tissue, with increased functionality in terms of synthesis of liver specific proteins after in vitro perfusion and in vivo subperitoneal transplantation in mice. Moreover, to establish immediate blood perfusion, an elastic layer was printed wrapping sacrificial ink to support the direct surgical anastomosis of the carotid artery to the jugular vein. Our findings highlight the support extended by vasculature network in soft hydrogels which helps to sustain the thick and dense cellularization in engineered tissues.

show abstract

Generation of model tissues with dendritic vascular networks via sacrificial laser-sintered carbohydrate templates

Cited by 113 publications

References 88 publications

Applications of Engineering Techniques in Microvasculature Design

Applications of Engineering Techniques in Microvasculature Design

Rapid synchronized fabrication of vascularized thermosets and composites

A novel method for generating 3D constructs with branched vascular networks using multi-materials bioprinting and direct surgical anastomosis

Contact Info

Product

Resources

About