Generation of monoclonal antibodies to characterize antigens of the nematode nippostrongylus brasiliensis

Bohn, A.; König, Wolfgang

doi:10.1016/s0176-6724(86)80068-2

Cited by 4 publications

(3 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Wistar rats and Balb/c mice were infected with 3,(XX) or 750 third stage (L3) larvae, respectively, as described previously [13], Reinfec tion of the animals was carried out 4 weeks later.…”

Section: Nb Infection Cyclementioning

confidence: 99%

“…Six to 8 days after the infection the feces of the rats were collected and killed to remove the small intestine. L3 larvae and adult worms were isolated as described elsewhere [13].…”

Section: Isolation O F L3 Larvae and Adult Wormsmentioning

confidence: 99%

“…The IgE production of B cells was enhanced only by WH/cytokines or when T cells were present. Individual antigen fractions (WH 5,8,9,10,13) increased the IgE production, which was enhanced in the presence of IL-4 2-to 3.5-fold) but had no significant effects on the IgG production. …”

mentioning

confidence: 91%

See 2 more Smart Citations

Effect of Antigens from <i>Nippostrongylus brasiliensis</i> and Cytokines on the Ongoing IgE Synthesis in vitro

Stephan¹,

König²

1992

Int Arch Allergy Immunol

View full text Add to dashboard Cite

The modulation of an ongoing IgE-mediated immune response induced by the infection of mice with the nematode Nippostrongylus brasiliensis (N.b.) was investigated in vitro. In previous experiments antigens derived from the homogenate of adult worms (WH) and third stage larvae (LH) were characterized by immunoblotting. Our results demonstrate that only antigens of the WH were recognized by IgE antibodies. The effect of worm antigens and cytokines (IL-2, IL·4, IL-5, IFNγ) on the IgE and IgG synthesis in different culture systems was studied. The IgG synthesis of B cells was stimulated by WH or WH/cytokines. The IgE production of B cells was enhanced only by WH/cytokines or when T cells were present. Individual antigen fractions (WH 5, 8, 9,10,13) increased the IgE production, which was enhanced in the presence of IL·4 2- to 3.5-fold) but had no significant effects on the IgG production.

show abstract

Section: Nb Infection Cyclementioning

confidence: 99%

“…Six to 8 days after the infection the feces of the rats were collected and killed to remove the small intestine. L3 larvae and adult worms were isolated as described elsewhere [13].…”

Section: Isolation O F L3 Larvae and Adult Wormsmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Antigens from <i>Nippostrongylus brasiliensis</i> and Cytokines on the Ongoing IgE Synthesis in vitro

Stephan¹,

König²

1992

Int Arch Allergy Immunol

View full text Add to dashboard Cite

show abstract

Characterization of antigens of the nematodeNippostrongylus brasiliensis by monoclonal antibodies

Bohn

König

1985

Z. Parasitenkd.

View full text Add to dashboard Cite

Hybridomas secreting monoclonal antibodies to Nippostrongylus brasiliensis antigens were generated by hybridization of IR983F myeloma cells with spleen cells from Lou/M/Wol rats infected with living third-stage larvae. Antibodies specific either for larval or worm antigens were identified by enzyme-linked immunosorbent assays with Nippostrongylus brasiliensis fragments, homogenates and secretions as antigens. The results demonstrate that all antibodies which recognized larval antigens (38 antibodies) also reacted with worm surfaces. Ten antibodies were specific only for worm antigens. Ten antibodies reacted with worm homogenate, three antibodies recognized components of worm secretion and 17 antibodies combined with acetylcholinesterase. The epitope specificity was investigated by the capacity of various glycosides, aminoacids, N-acetylneuraminic acid and phosphorylcholine to inhibit the binding to worm fragments. The analysis revealed that alpha-methylglucoside, alpha-methylmannoside, N-acetylglucosamine, N-acetylgalactosamine, fucose and the amino acids leucine, phenylalanine, tyrosine, serine, tryptophan did not combine with the antigen-binding sites of the antibodies. Proline, arginine and histidine, however, displayed inhibitory effects. With N-acetylneuraminic acid as inhibitor three groups of antibodies could be discriminated. At a concentration of 10-20 mM, phosphorylcholine was a potent inhibitor for all antibodies.

show abstract

Proteins ofNippostrongylus brasiliensis analyzed by immunoblotting

1989

View full text Add to dashboard Cite

Antigenic proteins were characterized by the immunoblotting technique with sera from rats and mice after infection as well as hyperimmune sera. The immune response of infected animals was mainly directed toward five proteins of adult worms (190, 118, 110, 98, and 52 kDa) and four proteins of the third larval stage (L3; 92, 78, 58, and 24 kDa). The immunoblots indicated that stage-specific proteins of the homogenates were recognized. Three stage-specific proteins of L3 larvae (150, 135, and 125 kDa) and three proteins typical to the adult worm (100, 82, and 67 kDa) were identified. The majority of the worm proteins elicited an IgG response. IgE synthesis was induced by living and dead parasites and was directed towards four proteins (190, 150, 125, and 98 kDa). Three proteins additionally induced an IgG or IgM antibody response. The immune response as shown by the immunoblotting technique seems to be directed towards (1) antigens that are present for the duration of an infection and (2) stage-specific antigens that are expressed for only a short time during the life cycle of the parasite.

show abstract

Generation of monoclonal antibodies to characterize antigens of the nematode nippostrongylus brasiliensis

Cited by 4 publications

References 13 publications

Effect of Antigens from <i>Nippostrongylus brasiliensis</i> and Cytokines on the Ongoing IgE Synthesis in vitro

Effect of Antigens from <i>Nippostrongylus brasiliensis</i> and Cytokines on the Ongoing IgE Synthesis in vitro

Characterization of antigens of the nematodeNippostrongylus brasiliensis by monoclonal antibodies

Proteins ofNippostrongylus brasiliensis analyzed by immunoblotting

Contact Info

Product

Resources

About