We show that a high-fidelity quantum gates to implement non-adiabatic holonomic quantum computation under electron spin states in Nitrogen-Vacancy(N V ) centers, providing an extensible experimental platform that has the potential for room-temperature quantum computing, which has increased attention recent years. Compared with the previous method, we can implement both the one-and two-qubit gates by varying the amplitude and phase of the microwave pulse applied to control the non-Abelian geometric phase acquired by N V centers. We also find that our proposed scheme may be implemented in the current experiment to discuss the gate fidelity with the experimental parameters. Therefore, the scheme adopts a new method to achieve high-fidelity non-adiabatic holonomic quantum computation.