Generation of non-toxic, chemical functional bio-polymer for desalination, metal removal and antibacterial activities from animal meat by-product

Etemadzadeh, Shekoofeh Sadat; Emtiazi, Giti

doi:10.1007/s13197-020-04525-z

Cited by 6 publications

(2 citation statements)

References 18 publications

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“…After suspending the protein precipitates (0%, 0.05%, and 0.1% lead(II) nitrate) in phosphate‐buffered saline (PBS), equal concentrations of protein were produced (checked with Bradford protein assay and the concentration of protein was 25 µg ml −1 ) and loaded on the polyacrylamide gel (12% separating gel and 5% stacking gels). The samples were not treated with 2‐mercaptoethanol (2‐ME) or heat before electrophoresis and the voltage was about 100 V. The gel was stained by Coomassie Brilliant Blue R‐250 and destained with methanol/acetic acid/water (45:10:45) (the experiment was repeated three times) [24].…”

Section: Methodsmentioning

confidence: 99%

Expression regulation of chitin‐binding protein and metal‐binding peptide in new Bacillus velezensis: MALDI‐TOF MS/MS analysis

Etemadzadeh

Emtiazi

2021

J Basic Microbiol

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Metallothionein and metal‐binding peptides are small cysteine‐rich proteins produced by different organisms in stress conditions. In this study, the metal‐binding peptide was detected in extracellular proteins of a new Bacillus velezensis strain, isolated from metal contaminated soil, and grown on the lead‐enriched medium, for the first time. The presence of sulfide peptide was assayed by two simple tests (lead sulfide and Ellman's reagent test) for preliminary, and subsequently confirmed using polyacrylamide gel electrophoresis at media with different lead concentrations that the low‐molecular‐weight protein fragments (≈10 kDa) were observed while none were detected in the medium containing sodium chloride or calcium salt. The amino acids of the observed fragments were analyzed by matrix‐assisted laser desorption ionization time‐of‐flight tandem mass spectrometry (MALDI‐TOF MS/MS). Also, the metal adsorption was confirmed using sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS‐PAGE) by staining with chromium solution. The results showed that the putative sulfide peptide is metallothionein, which is induced in stress conditions. It was interesting that in all SDS profiles, one protein fragment (≈18 kDa) was inhibited in lead‐enriched media. The data from MALDI‐TOF MS/MS analysis showed that this fraction was a chitin‐binding protein whose production was regulated by metal contamination. It is anticipated that these two proteins regulate the toxicity of lead.

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Section: Methodsmentioning

confidence: 99%

Expression regulation of chitin‐binding protein and metal‐binding peptide in new Bacillus velezensis: MALDI‐TOF MS/MS analysis

Etemadzadeh

Emtiazi

2021

J Basic Microbiol

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“…The purpose of the study of the use of functional ingredients in canned meat production is to analyse the use of functional ingredients in meat canning, to analyse technological strategies aimed at reducing health-related risk factors, to promote the beneficial effects of meat on health. Technological strategies (Etemadzadeh & Emtiazi, 2021;Sun et al, 2021;Yayuan et al, 2022) are mainly associated with an increase in the presence of useful functional ingredients in canned meat products, and with the restriction of those functional ingredients that have negative health effects.…”

Section: Introductionmentioning

confidence: 99%

Application of functional ingredients in canned meat production

et al. 2022

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A comprehensive literature search was conducted. An extended search was performed, including a targeted search on the websites of a number of intergovernmental and non-governmental organisations. All links identified in databases and search engines were uploaded to the Covidence systematic review tool for automatic deduplication and blind screening. The generalised material of the analysis represents a new typological basis of the market strategy for the analysis of the practices used by the dominant producers in the meat canning industry. Only a limited number of papers note the possible benefits of using functional ingredients in canned meat for human health, therefore further studies are required that would provide convincing evidence of the benefits of functional ingredients. As scientific evidence increases, researchers and meat producers should make more efforts to inform and educate consumers about the health benefits of functional ingredients used in meat canning.

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Production of biosurfactant by salt-resistant Bacillus in lead-supplemented media: application and toxicity

2023

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Generation of non-toxic, chemical functional bio-polymer for desalination, metal removal and antibacterial activities from animal meat by-product

Cited by 6 publications

References 18 publications

Expression regulation of chitin‐binding protein and metal‐binding peptide in new Bacillus velezensis: MALDI‐TOF MS/MS analysis

Expression regulation of chitin‐binding protein and metal‐binding peptide in new Bacillus velezensis: MALDI‐TOF MS/MS analysis

Application of functional ingredients in canned meat production

Production of biosurfactant by salt-resistant Bacillus in lead-supplemented media: application and toxicity

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