Generation of oxygen radicals in hemocytes of the snail Lymnaea stagnalis in relation to the rate of phagocytosis

Adema, Coen M.; Deutekom-Mulder, E.C. van; Knaap, W.P.W. van der; Meuleman, Elisabeth A.; Sminia, T.

doi:10.1016/0145-305x(91)90043-x

Cited by 68 publications

(21 citation statements)

References 24 publications

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“…) and/or nitric oxides (Anderson 1994;Bettencourt et al 2007;Husmann et al 2011), which are produced during the phagocytosis of particles and invading pathogens (Adema et al 1991). The observed increase in Le-catalase expression indicates enhanced intracellular H 2 O 2 generation, which can activate further the immune response qRT-PCR in different L. elliptica size/age groups individuals (small and large) sampled after 23 days of "food" (F) and "starvation" (S) treatment and experiencing acute (2 days after infliction) injury (S/I and F/I).…”

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confidence: 99%

Age-dependent expression of stress and antimicrobial genes in the hemocytes and siphon tissue of the Antarctic bivalve, Laternula elliptica, exposed to injury and starvation

Husmann

Abele

Rosenstiel

et al. 2014

Cell Stress and Chaperones

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Increasing temperatures and glacier melting at the Western Antarctic Peninsula (WAP) are causing rapid changes in shallow coastal and shelf systems. Climate change-related rising water temperatures, enhanced ice scouring, as well as coastal sediment runoff, in combination with changing feeding conditions and microbial community composition, will affect all elements of the nearshore benthic ecosystem, a major component of which is the Antarctic soft-shelled clam Laternula elliptica. A 454-based RNA sequencing was carried out on tissues and hemocytes of L. elliptica, resulting in 42,525 contigs, of which 48 % was assigned putative functions. Changes in the expression of putative stress response genes were then investigated in hemocytes and siphon tissue of young and old animals subjected to starvation and injury experiments in order to investigate their response to sedimentation (food dilution and starvation) and iceberg scouring (injury). Analysis of antioxidant defense (Le-SOD and Lecatalase), wound repair (Le-TIMP and Le-chitinase), and stress and immune response (Le-HSP70, Le-actin, and Letheromacin) genes revealed that most transcripts were more clearly affected by injury rather than starvation. The upregulation of these genes was particularly high in the hemocytes of young, fed individuals after acute injury. Only minor changes in expression were detected in young animals under the selected starvation conditions and in older individuals. The stress response of L. elliptica thus depends on the nature of the environmental cue and on age. This has consequences for future population predictions as the environmental changes at the WAP will differentially impact L. elliptica age classes and is bound to alter population structure.

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confidence: 99%

Age-dependent expression of stress and antimicrobial genes in the hemocytes and siphon tissue of the Antarctic bivalve, Laternula elliptica, exposed to injury and starvation

Husmann

Abele

Rosenstiel

et al. 2014

Cell Stress and Chaperones

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“…The collected hemocytes were resuspended in sterile snail saline (5 mM HEPES, 3.7 mM NaOH, 36 mM NaCl, 2 mM KCl, 2 mM MgCl2 N 2 H2O, and 4 mM CaCl 2 N 2 H 2 O at pH 7.8) [17] and stored at 48C.…”

Section: Methodsmentioning

confidence: 99%

Sodium Arsenite Induced Alteration of Hemocyte Density of Lamellidens marginalis – An Edible Mollusk from India

Chakraborty

Ray

Ray³

2008

CLEAN Soil Air Water

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Lamellidens marginalis (Molluska Bivalvia Eulamellibranchiata) is an important member of the freshwater ecosystem of India, which is sustained by filtering phytoplankton, bacteria and other particulate organic matter from the available water. Hemocytes, the circulating blood cells of bivalves, function as immunological effector cells under exposure to toxins and/or parasites. The hemocytes of Lamellidens marginalis have been identified morphologically and enumerated as being qualified for use as a biomarker to analyze the threat of freshwater contamination by sublethal concentrations of sodium arsenite, a natural pollutant of freshwater ecosystems. Diverse subpopulations of hemocytes were identified as blast-like cells, granulocytes, agranulocytes, hyalinocytes and asterocytes. Supression in the total count of hemocytes and blast-like cells was recorded under the exposure of all the concentrations of sodium arsenite tested. Sodium arsenite elevated the relative density of granulocytes, hyalinocytes, and asterocytes. Partial restoration of the total count of the hemocytes was recorded after the post-treated animals were maintained in arsenic-free water for periods of 15 and 30 days. The data predict a shift in the immunological parameters of this bivalve in arsenic contaminated environments. The present study is aimed at quantifying the arsenic induced stress in Lamellidens marginalis and establishing the hemocyte density as a biomarker of aquatic pollution in selected geographical regions of India.

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“…Snail saline buffer (SSB; 5 mM HEPES, 3.7 M NaOH, 36 mM NaCl, 2 mM KCl, 2 mM MgCl 2 , 4 mM CaCl2, 2 mM EDTA when needed; Adema et al 1991), was adjusted to pH 7.8. TBS buffer (0.6 M NaCl, 4.8 M TRIS) was adjusted to pH 7.5.…”

Section: Chemicals and Buffersmentioning

confidence: 99%

Effect of a toxicant on phagocytosis pathways in the freshwater snail Lymnaea stagnalis

Russo¹,

Mathieu²,

Brehélin

2008

Cell Tissue Res

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The disturbance of plasma membrane carbohydrates and of lipopolysaccharide (LPS) ligands in relation to cytoskeletal transformations of haemocytes has been investigated after chronic exposure of pond snails (Lymnaea stagnalis) to the peroxidizing toxicant fomesafen. Neither of the two lectins used (concanavalin A and wheat germ agglutinin) showed any binding modification after incubation of the snails in the presence of the toxicant. However, after exposure of the snails to fomesafen, a clear and persistent reduction in LPS labelling of haemocytes occurred. The actin cytoskeleton of the same cells also appeared to be sensitive to the toxicant. The reduction in LPS-binding sites was related to actin staining, leading to the hypothesis that LPS ligands and actin could be similarly modulated by the toxicant. Damaged cells showed non-adherent membrane portions with reduced filopodial extrusions, exhibiting a smooth surface free of microvilli. These changes could lower the spreading and adhesion of the cells and could therefore account for the loss in their phagocytic capabilities.

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Generation of oxygen radicals in hemocytes of the snail Lymnaea stagnalis in relation to the rate of phagocytosis

Cited by 68 publications

References 24 publications

Age-dependent expression of stress and antimicrobial genes in the hemocytes and siphon tissue of the Antarctic bivalve, Laternula elliptica, exposed to injury and starvation

Age-dependent expression of stress and antimicrobial genes in the hemocytes and siphon tissue of the Antarctic bivalve, Laternula elliptica, exposed to injury and starvation

Sodium Arsenite Induced Alteration of Hemocyte Density of Lamellidens marginalis – An Edible Mollusk from India

Effect of a toxicant on phagocytosis pathways in the freshwater snail Lymnaea stagnalis

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