Generation of Polypeptide-Templated Gold Nanoparticles using Ionizing Radiation

Walker, Candace R.; Pushpavanam, Karthik; Nair, Divya Geetha; Potta, Thrimoorthy; Sutiyoso, Caesario; Kodibagkar, Vikram D.; Sapareto, Stephen A.; Chang, John; Rege, Kaushal

doi:10.1021/la400567d

Cited by 21 publications

(22 citation statements)

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“…aliphatic surfactants) to engender the formation of plasmonic (gold and gold-silver) nanoparticles from their respective metal ion formulations. 12,[18][19][20] The formation of plasmonic nanoparticles in liquid or gel formulations is accompanied by a visible color change which is distinct from the original metal ion formulation, which is colorless. Here, we report, for the first time, a colorimetric approach for the rapid detection of therapeutic levels (0-3 Gy RBE ) of proton irradiation.…”

Section: Introductionmentioning

confidence: 99%

Hydrogel Nanosensors for Colorimetric Detection and Dosimetry in Proton Beam Radiotherapy

Inamdar

Pushpavanam

Lentz

et al. 2018

ACS Appl. Mater. Interfaces

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Proton beam therapy (PBT) is a state-of-the-art radiotherapy treatment approach that uses focused proton beams for tumor ablation. A key advantage of this approach over conventional photon radiotherapy (XRT) is the unique dose deposition characteristic of protons, which results in superior healthy tissue sparing. This results in fewer unwanted side effects and improved outcomes for patients. Currently available dosimeters are intrinsic, complex, and expensive and are not routinely used to determine the dose delivered to the tumor. Here, we report a hydrogel-based plasmonic nanosensor for detecting clinical doses used in conventional and hyperfractionated proton beam radiotherapy. In this nanosensor, gold ions, encapsulated in a hydrogel, are reduced to gold nanoparticles following irradiation with proton beams. Formation of gold nanoparticles renders a color change to the originally colorless hydrogel. The intensity of the color can be used to calibrate the hydrogel nanosensor in order to quantify different radiation doses employed during proton treatment. The potential of this nanosensor for clinical translation was demonstrated using an anthropomorphic phantom mimicking a clinical radiotherapy session. The simplicity of fabrication, detection range in the fractionated radiotherapy regime, and ease of detection with translational potential makes this a first-in-kind plasmonic colorimetric nanosensor for applications in clinical proton beam therapy.

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Section: Introductionmentioning

confidence: 99%

Hydrogel Nanosensors for Colorimetric Detection and Dosimetry in Proton Beam Radiotherapy

Inamdar

Pushpavanam

Lentz

et al. 2018

ACS Appl. Mater. Interfaces

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“…The small size as well as low yields of nanoparticles formed under these conditions explain the absence of the characteristic color associated with the gel. 19 With increasing time (>20 min), a maroon color, indicative of some nanoparticle formation (average diameter ≈ 30 nm as determined by TEM) was seen. The growth of gold nanoparticles likely progresses because of the subsequent reaction of unreacted free chloroaurate ions in the hydrogel with the existing gold nanoparticles (<2 nm).…”

Section: ■ Results and Discussionmentioning

confidence: 59%

Radiation-Responsive Amino Acid Nanosensor Gel (RANG) for Radiotherapy Monitoring and Trauma Care

et al. 2021

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Accurate detection of doses is critical for the development of effective countermeasures and patient stratification strategies in cases of accidental exposure to ionizing radiation. Existing detection devices are limited by high fabrication costs, long processing times, need for sophisticated detection systems, and/or loss of readout signal over time, particularly in complex environments. Here, we describe fundamental studies on amino acid-facilitated templating of gold nanoparticles following exposure to ionizing radiation as a new colorimetric approach for radiation detection. Tryptophan demonstrated spontaneous nanoparticle formation, and parallel screening of a library of amino acids and related compounds led to the identification of lead candidates, including phenylalanine, which demonstrated an increase in absorbance at wavelengths typical of gold nanoparticles in the presence of ionizing radiation (X-rays). Evaluation of screening, i.e., absorbance data, in concert with chemical informatics modeling led to the elucidation of physicochemical properties, particularly polarizable regions and partial charges, that governed nanoparticle formation propensities upon exposure of amino acids to ionizing radiation. NMR spectroscopy revealed key roles of amino and carboxy moieties in determining the nanoparticle formation propensity of phenylalanine, a lead amino acid from the screen. These findings were employed for fabricating radiationresponsive amino acid nanosensor gels (RANGs) based on phenylalanine and tryptophan, and efficacy of RANGs was demonstrated for predicting clinical doses of ionizing radiation in anthropomorphic thorax phantoms and in live canine patients undergoing radiotherapy. The use of biocompatible templating ligands (amino acids), rapid response, simplicity of fabrication, efficacy, ease of operation and detection, and long-lasting readout indicate several advantages of the RANG over existing detection systems for monitoring radiation in clinical radiotherapy, radiological emergencies, and trauma care.

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“…These species reduce the metal ions to its zerovalent state eventually giving rise to nanoparticles. In our initial work, cysteine‐containing elastin‐like polypeptides were used as potential capping agents for gold nanoparticles formed by the irradiation of an aqueous gold salt mixture . We observed accelerated nanoparticle formation with shorter‐chain ELP (21 kDa) when compared to longer‐chain ELP (42 kDa), which is likely due to reduced steric hindrance in presence of the larger polypeptide.…”

Section: Nanoparticle Systemsmentioning

confidence: 89%

Molecular and Nanoscale Sensors for Detecting Ionizing Radiation in Radiotherapy

2016

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Ever since the discovery of radioactivity,b eneficial use of ionizing radiation has been pursued for the betterment of human health. In particular, fractionated radiotherapy is commonlyu sed in the clinic fora blation of malignant tumors. Use of higher radiation dose fractionsa nd complex dose trajectories necessitate measurements of radiation dose delivered to the target tissue and surrounding tissues in order to ensure patient safety.T raditional dosimeters including polymer gel dosimeters, radiochromic films and metal-oxide semiconductor field effect transistors (MOSFETs) suffer from limitations, whichc omplicate their day-to-day use in the clinic. Molecular and nanoscale systems offer great potentialf or the development of effective sensors of ionizingr adiation,w hich can lead to quantitative dosimeters in biological settings. Thisr eview discusses recent developments based on organic and inorganic molecular and nanoscale dosimeters including quantum dots,p olymers and plasmonic nanoparticles as platforms for radiation sensing. Potential advantages and challenges of translating these technologiestoc linical applicationsare also discussed.

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Generation of Polypeptide-Templated Gold Nanoparticles using Ionizing Radiation

Cited by 21 publications

References 50 publications

Hydrogel Nanosensors for Colorimetric Detection and Dosimetry in Proton Beam Radiotherapy

Hydrogel Nanosensors for Colorimetric Detection and Dosimetry in Proton Beam Radiotherapy

Radiation-Responsive Amino Acid Nanosensor Gel (RANG) for Radiotherapy Monitoring and Trauma Care

Molecular and Nanoscale Sensors for Detecting Ionizing Radiation in Radiotherapy

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