Generation of reactive oxygen species by Kupffer cells and blood monocytes during intestinal amebiasis in guinea pigs

Virk, K. J.; Ganguly, Nirmal Kumar; Mahajan, Rohit; Bhushnurmath, S R; Dilawari, J. B.

doi:10.1007/bf02782957

Cited by 3 publications

(1 citation statement)

References 24 publications

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“…Also, macrophage down‐regulating cytokines (IL‐10 and TGF‐β) expressed significantly higher in the symptomatic group, and thus might be playing modulatory roles in amoebic infections. In addition, these cells which are stimulated might lead to release of enzymes (26,27) or additional cytokines, which could lead to tissue damage, as these are non‐specific and do not distinguish between host tissue and the amoebae as far as killing is concerned.…”

Section: Discussionmentioning

confidence: 99%

Cytokine mRNA expressions in symptomatic vs. asymptomatic amoebiasis patients

Bansal¹,

Sehgal²,

Chawla

et al. 2005

Parasite Immunology

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Infection with Entamoeba histolytica results in high mortality worldwide. Studies on the cytokine response in symptomatic and asymptomatic amoebiasis (caused by E. histolytica, the pathogenic species and E. dispar, the non-pathogenic species) subjects and their correlation with symptomatology are lacking. The present study reports the cytokine response (TNF-alpha, IFN-gamma, IL-4, IL-10 and TGF-beta) in such subjects as measured by RT-PCR. The results showed significantly (< 0.05) higher expressions of IL-10 and TGF-beta in the symptomatic group as compared to the asymptomatic and healthy controls. The cytokine profile indicated the role of suppressive immune response in symptomatic amoebiasis patients.

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Section: Discussionmentioning

confidence: 99%

Cytokine mRNA expressions in symptomatic vs. asymptomatic amoebiasis patients

Bansal¹,

Sehgal²,

Chawla

et al. 2005

Parasite Immunology

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Papers from Abroad

et al. 1990

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The capacity of Kupffer cells and blood monocytes to release free oxygen radicals was studied by chemiluminescence (CL) response during hepatic amebiasis in guinea pigs. Guinea pigs infected with Entamoeba histolytica intramesenterically were sacrificed on days 0, 2, 5 and 8 post-infection. Hepatic lesions were graded I-IV. A significant increase in the CL response was observed from day 2 post-infection and it increased with the progress of infection. Maximum increase was observed on the 8th post infection day. Animals with grade III or IV hepatic lesions had significantly elevated CL response. The degree of hepatic lesions correlated well with the CL response (P less than 0.01). It is postulated that tissue damage during hepatic amoebiasis may be mediated through enhanced release of free oxygen radicals by Kupffer cells and blood monocyte.

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Kupffer cell functions during intestinal amoebiasis in guinea pigs

Virk

Ganguly

Prasad

et al. 1990

J of Gastro and Hepatol

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Kupffer cells play an important role in all alimentary tract infections. Their role in intestinal amoebiasis is not clear. Hence, the present study examined Kupffer cell functions--phagocytic capacity and levels of a key lysosomal enzyme, N-acetyl-beta-glucosaminidase -- in guinea pigs infected with Entamoeba histolytica intracaecally. Animals were sacrificed on days 0, 3, 7, 14 and 21 post-infection. During intestinal amoebiasis the phagocytic capacity in Kupffer cells was depressed, whereas lysosomal enzyme levels were highly elevated. Maximum alteration in Kupffer cell functions was observed on the 14th post-infection day (P less than 0.01). Animals which survived until 21 days post-infection did not show any significant (P greater than 0.05) change in their Kupffer cell functions compared with controls and day zero values. Phagocytic capacity was inversely correlated with severity of caecal lesions. In contrast, enzyme levels were directly correlated with degree of caecal lesions. Similar trends were observed in blood monocytes. The depressed phagocytic capacity of mononuclear phagocytes and the increased enzyme release by these cells accompanying intestinal amoebic infection may contribute to the establishment of the infection and the tissue damage that accompanies it.

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Generation of reactive oxygen species by Kupffer cells and blood monocytes during intestinal amebiasis in guinea pigs

Cited by 3 publications

References 24 publications

Cytokine mRNA expressions in symptomatic vs. asymptomatic amoebiasis patients

Cytokine mRNA expressions in symptomatic vs. asymptomatic amoebiasis patients

Papers from Abroad

Kupffer cell functions during intestinal amoebiasis in guinea pigs

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