Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells

Mahato, Sudipta; Agrawal, Trupti; Pidishetty, Divya; Maddileti, Savitri; Pulimamidi, Vinay Kumar; Mariappan, Indumathi

doi:10.3791/64509

Cited by 5 publications

(2 citation statements)

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“…The talks in this session were focussed on using iPSC-derived organoid models to understand brain and eye development, cell-fate regulation and disease pathophysiology ( Mahato et al, 2022 ; Sriram et al, 2020 ; Susaimanickam et al, 2017 ). Mimicking human neurological disorders in rodent models is often challenging due to the species-specific differences in the complexity of the human brain.…”

Section: Understanding Human Development and Disease Using Mps Modelsmentioning

confidence: 99%

New horizons of microphysiological systems: India forging its path in human-relevant research

Parvatam¹,

Mahadik²,

Banerjee³

et al. 2023

Biology Open

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The past decade has seen expeditious developments in our ability to grow and maintain a variety of human cells and tissues, with properties closely mimicking those in the human body. Prominent researchers and entrepreneurs from all over the world assembled in Hyderabad, India to discuss developments in this field that have not only aided fundamental understanding of organ development and disease processes but have served as good physiological models for toxicity testing and drug development. The speakers presented ingenious, cutting-edge technology and forward-thinking ideas. This report presents the salient aspects of their discussions, highlights the importance of identifying unmet needs, and discusses setting of standards that will help regulatory approvals as we move into a new era, with nominal animal use in research and effective drug discovery.

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Section: Understanding Human Development and Disease Using Mps Modelsmentioning

confidence: 99%

New horizons of microphysiological systems: India forging its path in human-relevant research

Parvatam¹,

Mahadik²,

Banerjee³

et al. 2023

Biology Open

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show abstract

“…Additionally, genetic abnormalities affect the development of patient-derived ROs with distinct diseases or variants. Mahato et al reported that the retinal forming efficiency of RP disease-specific iPSCs was identical to that of the healthy control cells; however, iPSCs with the RB1 −/− mutation failed to form eye field primordial structures [ 111 ]. The passage approach employing enzyme and manual purification was more effective than flow cytometry-based sorting for high-yield purification of functional RPE cells from diverse stem cell sources [ 112 ].…”

Section: Challenges In the Clinical Application Of Ipscs And Organoidsmentioning

confidence: 99%

Application of patient-derived induced pluripotent stem cells and organoids in inherited retinal diseases

Liang,

Sun,

Duan

et al. 2023

Stem Cell Res Ther

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Inherited retinal diseases (IRDs) can induce severe sight-threatening retinal degeneration and impose a considerable economic burden on patients and society, making efforts to cure blindness imperative. Transgenic animals mimicking human genetic diseases have long been used as a primary research tool to decipher the underlying pathogenesis, but there are still some obvious limitations. As an alternative strategy, patient-derived induced pluripotent stem cells (iPSCs), particularly three-dimensional (3D) organoid technology, are considered a promising platform for modeling different forms of IRDs, including retinitis pigmentosa, Leber congenital amaurosis, X-linked recessive retinoschisis, Batten disease, achromatopsia, and best vitelliform macular dystrophy. Here, this paper focuses on the status of patient-derived iPSCs and organoids in IRDs in recent years concerning disease modeling and therapeutic exploration, along with potential challenges for translating laboratory research to clinical application. Finally, the importance of human iPSCs and organoids in combination with emerging technologies such as multi-omics integration analysis, 3D bioprinting, or microfluidic chip platform are highlighted. Patient-derived retinal organoids may be a preferred choice for more accurately uncovering the mechanisms of human retinal diseases and will contribute to clinical practice.

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Generation of Leber congenital amaurosis, type 12 patient-specific induced pluripotent stem cell line (LVPEIi006-A), harboring a homozygous mutation in RD3

Mahato,

Maddileti,

Naik

et al. 2024

Stem Cell Research

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Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells

Cited by 5 publications

References 0 publications

New horizons of microphysiological systems: India forging its path in human-relevant research

New horizons of microphysiological systems: India forging its path in human-relevant research

Application of patient-derived induced pluripotent stem cells and organoids in inherited retinal diseases

Generation of Leber congenital amaurosis, type 12 patient-specific induced pluripotent stem cell line (LVPEIi006-A), harboring a homozygous mutation in RD3

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