Generation of site-specifically labelled fluorescent human XPA to investigate DNA binding dynamics during nucleotide excision repair

Kuppa, Sahiti; Corless, Elliot; Caldwell, Colleen C.; Spies, Maria; Antony, Edwin

doi:10.1016/j.ymeth.2024.02.006

Methods

2024

DOI: 10.1016/j.ymeth.2024.02.006

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Generation of site-specifically labelled fluorescent human XPA to investigate DNA binding dynamics during nucleotide excision repair

Sahiti Kuppa,

Elliot Corless,

Colleen C. Caldwell

et al.

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2024

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smCamera: all-in-one software package for single-molecule data acquisition and data analysis

Lee,

2024

J. Korean Phys. Soc.

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In the last decades, biological applications of single-molecule methods have grown rapidly and researchers with expertise in microscopy and instrumentation have adopted these techniques and advanced them even further. However, practicing single-molecule methods is still challenging for most because of the need for expensive equipments and instrumentation demands as well as complicated data acquisition and analysis workflow consisting of multiple steps using different software in each of them. smCamera is an all-in-one software package which aims to alleviate steep learning curves of the data acquisition and analysis. From recording movies through extraction of time series of single-molecule fluorescence resonance energy transfer (smFRET), everything can be done in a single program without any additional software or programming languages. Although smCamera has not been published before, it has been distributed to facilitate adaption of smFRET experiments and there has been requests for support for this software. We hope that this work answers questions from researchers using smCamera and also provides an opportunity to introduce and distribute smCamera for new users.

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smCamera: all-in-one software package for single-molecule data acquisition and data analysis

Lee,

2024

J. Korean Phys. Soc.

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Generation of site-specifically labelled fluorescent human XPA to investigate DNA binding dynamics during nucleotide excision repair

Cited by 1 publication

References 36 publications

smCamera: all-in-one software package for single-molecule data acquisition and data analysis

smCamera: all-in-one software package for single-molecule data acquisition and data analysis

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