Generation of Suppressor Cells in Mice Immunized With M Locus-Incompatible Lymphocytes

Matossian-Rogers, A; Festenstein, H.

doi:10.1097/00007890-197704000-00004

Cited by 16 publications

(7 citation statements)

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“…Immune suppression: (a) The tnost striking biological function of the Mis system is its suppressor properties, first observed by Lilliehook and co-workers (Lilliehook et al 1975). Subsequently, specific experiments using typed backcrosses and congenic strains in our group confirmed that mice preimmunised in vivo with Mis-incompatible cells not only suppressed anti-Mis' proliferative responses but also suppressed the cytotoxic potential against H-2 plus Mis incompatible cells in vitro (Matossian-Rogers & Festenstein 1976, 1977b. While the induction is highly specific for the Mls^ Lad, the effector phase is non-speciftc, the initial experiments were done ttsing strain combinations which are H-2-identical but differ for the Mis' determinant as well as other non-H-2 antigens.…”

Section: Tissue Distribution Studied By Alloimmune Mis' Vs Mis'" Intementioning

confidence: 80%

Mls and Tolerance

Festenstein

Kumura

Blasi

1989

Immunological Reviews

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Section: Tissue Distribution Studied By Alloimmune Mis' Vs Mis'" Intementioning

confidence: 80%

Mls and Tolerance

Festenstein

Kumura

Blasi

1989

Immunological Reviews

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“…The Mls locus is a mouse non-H-2 locus mapped on chromosome 1 (Festenstein et al, 1977) (Rollinghoff et at., 1975;Peck et al, 1977a), but cytostatic effector cells active against Mls" determinants on monocytes can be generated in mice by an in vivo immunization with Mlsa incompatible lymphocytes (Matossian-Rogers & Festenstein, 1977a). Furthermore, in vitro restimulation with Mlsa incompatible lymphocytes following in vivo immunization results in a decreased cytotoxic response against H-2 targets; this phenomenon has been attributed to the induction of suppressor cells (Matossian-Rogers & Festenstein, 1977b). Incompatibility for the Mls determinants was responsible neither for skin graft rejection nor for graft-versus-host reactions (GVHR) in the spleen assay after grafting normal lymphoid cells (Festenstein, 1976).…”

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confidence: 99%

INCOMPATIBILITY FOR OR PRE‐IMMUNIZATION AGAINST M1s DETERMINANTS DECREASES LETHAL GRAFT‐VERSUS‐HOST REACTION DEVELOPED ACROSS NON ‐ H ‐ 2 AND/OR H‐2 BARRIERS

Hallé-Pannenko

Festenstein

1981

Int J Immunogenetics

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SUMMARY The effect of incompatibility for Mls determinants was studied in lethal graft‐versus‐host reaction (GVHR) in the mouse. GVHR was induced in adult recipients of the following H‐2k strains: (AKR x B10.BR)F1 (MlsB/Mlsb); (C3H x B10.BR)F1 (Mlsc/Mlsb); (CBA/J x B10.BR)F1 (Mlsd/Mlsb) and (CBA/H x B10.BR)F1 (Mlsb). Recipient mice were heavily irradiated and grafted with bone marrow and spleen cells from H‐2 compatible B10.BR (H‐2k, Mlsb) or H‐2 incompatible B10.D2 and B10 donors were normal, while those from B10.BR donors were either normal or pre‐immunized against the recipient strains. In all experiments the survival of recipients with Mlsa/Mlsb and Mlsd/Mlsb phenotypes, and only in one experiment of those with Mlsc/Mlsb phenotype was greater and/or the survival time longer than that of recipients expressing only Mlsb. However, late deaths (> 120 days post grafting) observed after grafting of normal B10.BR cells were more frequent in Mlsd/Mlsb than in Mlsb strains. On the other hand, when B10.BR donor cells were pre‐immunized against H‐2k compatible (AKR x B10.BR)F1 (Mlsa/Mlsb) or (CBA/J x B10.BR)F1 (Mlsd/Mlsb) strains, the survival time of H‐2 incompatible (B10 x B10.BR)F1 (H‐2b/k, Mlsb) recipients was longer than when donor cells were pre‐immunized against (CBA/H x B10.BR)F1 (Mlsb) strain. We conclude that donor incompatibility for Mlsa or Mlsd or donor‐pre‐immunization against Mlsa or Mlsd exerts a protective effect on lethal GVHR developed across non‐H‐2 or H‐2 barriers; the protective effect of Mlsc is less efficient or absent. The Mls‐induced protective effect shows the following properties: (a) efficiency in vivo correlates with the capacity of the corresponding alleles to stimulate an in vitro MLR; (b) is efficient in either primary or secondary response to other minor antigens; (c) is not H‐2 restricted; (d) is nonspecific; (e) disappears late after grafting; (f) with respect to the genetic background, the early protective effect is followed, late after grafting, by an opposite effect which increases the mortality, suggesting that M/s locus determinants are capable of activating several cell populations with different biological functions.

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“…It has in fact been shown that a secondary response to non-H-2 alloantigens can be specifically suppressed by cells recovered also from a secondary MLR to non-H-2 alloantigen but not by cells from a primary MLR (38). Immunization of mice with Mls-disparate spleen cells results in the production in the lymph nodes, of suppressive activity which effects MLR, in vitro Tc responses and polyclonal proliferative responses (39)(40)(41). The mechanism of this in vivo generated suppression may be the same as the in vitro generated suppression described here.…”

Section: Discussionmentioning

confidence: 99%

Suppressor T cells activated in a primary in vitro response to non-major histocompatibility alloantigens.

Macphail¹,

Stutman²

1982

The Journal of Experimental Medicine

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Normal mouse spleen cells are not capable of mounting a primary cytotoxic T lymphocyte (Tc) response to non-H-2 alloantigens in vitro, although a good secondary H-2-restricted response is observable after in vivo immunization of the responder animals. Suppressor cells are generated in such a primary responses provided a Mls incompatibility exists between the responder and stimulator. These suppressors are not antigen specific, are Thy-1+, Lyt-1+, 2-, I-J-, and are highly radiosensitive. The suppressor cell precursors in normal spleen express the same phenotype. These suppressor cells are probably implicated in the lack of a primary Tc response in a primary mixed lymphocyte reaction across non-H-2 incompatibilities that include an Mls difference.

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Generation of Suppressor Cells in Mice Immunized With M Locus-Incompatible Lymphocytes

Cited by 16 publications

References 0 publications

Mls and Tolerance

Mls and Tolerance

INCOMPATIBILITY FOR OR PRE‐IMMUNIZATION AGAINST M1s DETERMINANTS DECREASES LETHAL GRAFT‐VERSUS‐HOST REACTION DEVELOPED ACROSS NON ‐ H ‐ 2 AND/OR H‐2 BARRIERS

Suppressor T cells activated in a primary in vitro response to non-major histocompatibility alloantigens.

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