Generation of the Seizure Discharge in Thin Sections From the Guinea Pig Brain in Chloride-Free Medium in Vitro

Yamamoto, Chosaburo; Kawai, Nobufumi

doi:10.2170/jjphysiol.18.620

Cited by 49 publications

(14 citation statements)

References 14 publications

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“…Our results resembled those reported by Yamamoto & Kawai (1968), who blocked GABAergic inhibition with Cl --free medium. Baclofen failed to inhibit any aspect of this response, even at a concentration which usually totally suppressed afterdischarge in areas CA1 and CA3.…”

Section: Discussionsupporting

confidence: 92%

Anticonvulsant‐like actions of baclofen in the rat hippocampal slice

Ault

Nadler

1983

British J Pharmacology

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Section: Discussionsupporting

confidence: 92%

Anticonvulsant‐like actions of baclofen in the rat hippocampal slice

Ault

Nadler

1983

British J Pharmacology

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“…[Cl-] was reduced below 40 mm (by substitution with isethionate or propionate ions, see Methods) the membrane potential became depolarized by 10-50 mV and unstable. 'Seizure discharges' were seen as described previously (Richards & Sercombe, 1968a;Yamamoto & Kawai, 1968 Effect of temperature Low incubation temperatures (25 00) were used in the present study since previous experiments have indicated that this helps to maintain the long-term viability of thick slices in vitro (Harvey et al 1974). The effects of short-term changes in incubation temperature on the properties of olfactory cortical neurones is shown in Fig.. Leusen, 1972;Katzman, 1976).…”

Section: Properties Of Olfactory Cortex Neuronesmentioning

confidence: 93%

Electrical properties of neurones in the olfactory cortex slice in vitro.

Scholfield¹

1978

The Journal of Physiology

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SUMMARY1. Slices of guinea-pig olfactory cortex were maintained in vitro. Electrical properties of neurones in the prepyriform region were studied using single high resistance glass micro-electrodes filled with potassium acetate, connected to a resistancecompensating circuit to allow passage of current through the electrode.2. Neurones showed a high, stable resting membrane potential (75-4 + 2-7 mV, mean + S.D.; n = 47). Input resistance measured with small depolarizing currents varied over a range of 9-280 MD. The time constant for decay of depolarizing potentials was 19-4 + 7-5 msec (mean + S.D.). The relationship between membrane potential and [K]0.t accorded with a valuefor PNa:PK of 0 02.4. Depolarization produced repetitive action potentials (maximum frequency of 85 Hz) having peak amplitudes of + 16 to + 47 mV. The action potential was followed by a depolarizing after potential of about 20 mV positive to the membrane potential.5. In these and other respects, the prepyriform neurones appear to behave like most other neurones in the mammalian brain, after allowing for the more stable recording conditions in this preparation.

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“…in addition, the cells and fibres are in discrete layers within the dentate gyrus (Lomo, 1971, Nafstad, 1967. As the granule cells of the lower blade of the dentate gyrus are superficial, a convenient in vitro preparation of this area can be made by taking a tangential slice across the regio inferior of the hippocampus and then incubating this slice of tissue in a suitable environment (see Yamamoto & Kawai, 1968;Bliss & Richards, 1971). The preparation so made consists of a single densely packed layer of granule cells together with the terminal lengths of their afferent perforant path fibres.…”

Section: Methodsmentioning

confidence: 99%

The actions of volatile anaesthetics on synaptic transmission in the dentate gyrus.

Richards¹,

White²

1975

The Journal of Physiology

116

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SUMMARY1. The actions of four volatile anaesthetics on the evoked synaptic potentials of in vitro preparations of the hippocampus were examined.2. All four anaesthetics (ether, halothane, methoxyflurane and trichloroethylene) depressed the synaptic transmission between the perforant path and the granule cells at concentrations lower than those required to maintain anaesthesia in intact animals.3. The population excitatory post-synaptic potential (e.p.s.p.) and massed discharge of the cortical cells (population spike) were depressed at concentrations of the anaesthetics lower than those required to depress the compound action potential of the perforant path nerve fibres. None of the anaesthetics studied increased the threshold depolarization required for granule cell discharge. Furthermore, frequency potentiation of the evoked cortical e.p.s.p.s was not impaired by any of the anaesthetics studied.4. It is concluded that all four anaesthetics depress synaptic transmission in the dentate gyrus either by reducing the amount of transmitter released from each nerve terminal in response to an afferent volley, or by decreasing the sensitivity of the post-synaptic membrane to released transmitter or by both effects together.

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Generation of the Seizure Discharge in Thin Sections From the Guinea Pig Brain in Chloride-Free Medium in Vitro

Cited by 49 publications

References 14 publications

Anticonvulsant‐like actions of baclofen in the rat hippocampal slice

Anticonvulsant‐like actions of baclofen in the rat hippocampal slice

Electrical properties of neurones in the olfactory cortex slice in vitro.

The actions of volatile anaesthetics on synaptic transmission in the dentate gyrus.

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