Generation of three-dimensional multiple spheroid model of olfactory ensheathing cells using floating liquid marbles

Vadivelu, Raja; Ooi, Chin Hong; Yao, Rebecca; Velasquez, Johana Tello; Pastrana, Érika; Diaz-Nido, Javier; Lim, Filip; Ekberg, Jenny; Nguyen, Nam‐Trung; John, James St

doi:10.1038/srep15083

Cited by 123 publications

(128 citation statements)

References 41 publications

Supporting

Mentioning

124

Contrasting

Unclassified

Order By: Relevance

“…The control spheroids had the average migration of 478.5 μ m, while the majority of the tested materials did not allow for more than 100 μ m migration. Since it is generally established that thriving, robust cells migrate further and faster than the harmed cells, 55 this limited migration distance indicates a successful treatment. As seen in Figure 7, the control spheroid has long arms extending form it, but SPION and MB particle treatments block this extension.…”

Section: Resultsmentioning

confidence: 99%

Hydroxyapatite as a Vehicle for the Selective Effect of Superparamagnetic Iron Oxide Nanoparticles against Human Glioblastoma Cells

Pernal

Uskoković

2017

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Despite the early promises of magnetic hyperthermia (MH) as a method for treating cancer, it has been stagnating in the past decade. Some of the reasons for the low effectiveness of superparamagnetic nanoparticles (SPIONs) in MH treatments include (a) low uptake in cancer cells; (b) generation of reactive oxygen species that cause harm to the healthy cells; (c) undeveloped targeting potential; and (d) lack of temperature sensitivity between cancer cells and healthy cells. Here we show that healthy cells, including human mesenchymal stem cells (MSCs) and primary mouse kidney and lung fibroblasts, display an unfavorably increased uptake of SPIONs compared to human brain cancer cells (E297 and U87) and mouse osteosarcomas cells (K7M2). Hydroxyapatite (HAP), the mineral component of our bones, may offer a solution to this unfavorably selective SPION delivery. HAP nanoparticles are commended not only for their exceptional biocompatibility but also for the convenience of their use as an intracellular delivery agent. Here we demonstrate that dispersing SPIONs in HAP using a wet synthesis method could increase the uptake in cancer cells and minimize the risk to healthy cells. Specifically, HAP/SPION nanocomposites retain the superparamagnetic nature of SPIONs, increase the uptake ratio between U87 human brain cancer cells and human MSCs versus their SPION counterparts, reduce migration in a primary brain cancer spheroid model compared to the control, reduce brain cancer cell viability compared to the treatment with SPIONs alone, and retain the viability of healthy human MSCs. A functional synergy between the two components of the nanocomposites was established; as a result, the cancer versus healthy cell (U87/MSC) selectivity in terms of both the uptake and the toxicity was higher for the composite than for SPIONs or HAP alone, allowing it to be damaging to cancer cells and harmless to the healthy ones. The analysis of actin cytoskeleton order at the microscale revealed that healthy MSCs and primary cancer cells after the uptake of SPIONs display reduced and increased anisotropy in their cytoskeletal arrangement, respectively. In contrast, the uptake of SPION/HAP nanocomposites increased the cytoskeletal anisotropy of both the healthy MSCs and the primary cancer cells. In spite of the moderate specific magnetization of HAP/SPION nanohybrids, reaching 15 emu/g for the 28.6 wt % SPION-containing composite, the cancer cell treatment in an alternating magnetic field resulted in an intense hyperthermia effect that increased the temperature by ca. 1 °C per minute of exposure and reduced the cell population treated for 30 min by more than 50%, while leaving the control populations unharmed. These findings on nanocomposites of HAP and SPIONs may open a new avenue for cancer therapies that utilize MH.

show abstract

Section: Resultsmentioning

confidence: 99%

Hydroxyapatite as a Vehicle for the Selective Effect of Superparamagnetic Iron Oxide Nanoparticles against Human Glioblastoma Cells

Pernal

Uskoković

2017

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…A liquid marble can be driven across a liquid surface using thermocapillarity1718. Liquid marbles served as micro bioreactors for culturing cells1920212223. A number of excellent review papers provided a comprehensive summary on properties and applications of liquid marbles114242526.…”

mentioning

confidence: 99%

Measuring the Coefficient of Friction of a Small Floating Liquid Marble

Ooi

Nguyen

Evans

et al. 2016

Sci Rep

Self Cite

View full text Add to dashboard Cite

This paper investigates the friction coefficient of a moving liquid marble, a small liquid droplet coated with hydrophobic powder and floating on another liquid surface. A floating marble can easily move across water surface due to the low friction, allowing for the transport of aqueous solutions with minimal energy input. However, the motion of a floating marble has yet to be systematically characterised due to the lack of insight into key parameters such as the coefficient of friction between the floating marble and the carrier liquid. We measured the coefficient of friction of a small floating marble using a novel experimental setup that exploits the non-wetting properties of a liquid marble. A floating liquid marble pair containing a minute amount magnetite particles were immobilised and then released in a controlled manner using permanent magnets. The capillarity-driven motion was analysed to determine the coefficient of friction of the liquid marbles. The “capillary charge” model was used to fit the experimental results. We varied the marble content and carrier liquid to establish a relationship between the friction correction factor and the meniscus angle.

show abstract

“…There are several methods for cell spheroid formation other than the microfluidic approach including magnetic levitation [34], 3D-bioprinting [35], hydrophobic surfaces [36], matrix-on-top [37], matrix-embedded [38], polymeric aqueous two-phase system [39], floating liquid marbles [40], multiwell plates [41], bioreactor flasks [42], liquid overlay [43] and HD techniques [36]. Some of these techniques such as HD and multiwell plates are laborious while some others like 3D-bioprinting and magnetic levitation are costly and still lack the standard protocols.…”

Section: Conventional Methods For Spheroid Formationmentioning

confidence: 99%

Inventions and Innovations in Preclinical Platforms for Cancer Research

2018

View full text Add to dashboard Cite

Three-dimensional (3D) cell culture systems can be regarded as suitable platforms to bridge the huge gap between animal studies and two-dimensional (2D) monolayer cell culture to study chronic diseases such as cancer. In particular, the preclinical platforms for multicellular spheroid formation and culture can be regarded as ideal in vitro tumour models. The complex tumour microenvironment such as hypoxic region and necrotic core can be recapitulated in 3D spheroid configuration. Cells aggregated in spheroid structures can better illustrate the performance of anti-cancer drugs as well. Various methods have been proposed so far to create such 3D spheroid aggregations. Both conventional techniques and microfluidic methods can be used for generation of multicellular spheroids. In this review paper, we first discuss various spheroid formation phases. Then, the conventional spheroid formation techniques such as bioreactor flasks, liquid overlay and hanging droplet technique are explained. Next, a particular topic of the hydrogel in spheroid formation and culture is explored. This topic has received less attention in the literature. Hydrogels entail some advantages to the spheroid formation and culture such as size uniformity, the formation of porous spheroids or hetero-spheroids as well as chemosensitivity and invasion assays and protecting from shear stress. Finally, microfluidic methods for spheroid formation and culture are briefly reviewed.

show abstract

Generation of three-dimensional multiple spheroid model of olfactory ensheathing cells using floating liquid marbles

Cited by 123 publications

References 41 publications

Hydroxyapatite as a Vehicle for the Selective Effect of Superparamagnetic Iron Oxide Nanoparticles against Human Glioblastoma Cells

Hydroxyapatite as a Vehicle for the Selective Effect of Superparamagnetic Iron Oxide Nanoparticles against Human Glioblastoma Cells

Measuring the Coefficient of Friction of a Small Floating Liquid Marble

Inventions and Innovations in Preclinical Platforms for Cancer Research

Contact Info

Product

Resources

About