Generation of transplastomic lettuce with enhanced growth and high yield

Ichikawa, Yaka; Tamoi, Masahiro; Sakuyama, Harumi; Maruta, Takanori; Ashida, Hiroshi; Yokota, Akiho; Shigeoka, Shigeru

doi:10.4161/gmcr.1.5.14706

Cited by 41 publications

(30 citation statements)

References 18 publications

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“…In addition, the hexose, sucrose, and starch contents of the stem, root, and different leaves were in general higher in transplastomic plants than in non-transformed plants (at least during distinct stages of the diurnal cycle) (Yabuta et al 2008). In lettuce, photosynthetic capacity and productivity of the transplastomic plants increased 1.3-and 1.6-fold, respectively, when compared to non-transformed plants (Ichikawa et al 2010). These data support that plastid metabolic engineering of enzymes limiting photosynthesis may result in improved yield and productivity.…”

Section: Attempts To Influence Crop Productivitymentioning

confidence: 94%

“…On the basis of these results, transplastomic tobacco (Yabuta et al 2008) and lettuce plants (Ichikawa et al 2010) expressing cyanobacterial fructose-1,6/ sedoheptulose-1,7-bisphosphatase (FBP/SBPase) in the chloroplast have recently been generated, and both exhibited increased productivity. In tobacco plants, expression by three different plastid promoters caused a minimum of 2-or 3-fold increase in the enzyme activity, which resulted in up to 1.8-fold increase of the dry matter when compared to the nontransformed plants.…”

Section: Attempts To Influence Crop Productivitymentioning

confidence: 99%

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Transplastomic plants for innovations in agriculture. A review

Wani

Sah

Sági

et al. 2015

Agron. Sustain. Dev.

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Food production has to be significantly increased in order to feed the fast growing global population estimated to be 9.1 billion by 2050. The Green Revolution and the development of advanced plant breeding tools have led to a significant increase in agricultural production since the 1960s. However, hundreds of millions of humans are still undernourished, while the area of total arable land is close to its maximum utilization and may even decrease due to climate change, urbanization, and pollution. All these issues necessitate a second Green Revolution, in which biotechnological engineering of economically and nutritionally important traits should be critically and carefully considered. Since the early 1990s, possible applications of plastid transformation in higher plants have been constantly developed. These represent viable alternatives to existing nuclear transgenic technologies, especially due to the better transgene containment of transplastomic plants. Here, we present an overview of plastid engineering techniques and their applications to improve crop quality and productivity under adverse growth conditions. These applications include (1) transplastomic plants producing insecticidal, antibacterial, and antifungal compounds. These plants are therefore resistant to pests and require less pesticides. (2) Transplastomic plants resistant to cold, drought, salt, chemical, and oxidative stress. Some pollution tolerant plants could even be used for phytoremediation. (3) Transplastomic plants having higher productivity as a result of improved photosynthesis. (4) Transplastomic plants with enhanced mineral, micronutrient, and macronutrient contents. We also evaluate field trials, biosafety issues, and public concerns on transplastomic plants. Nevertheless, the transplastomic technology is still unavailable for most staple crops, including cereals. Transplastomic plants have not been commercialized so far, but if this crop limitation were overcome, they could contribute to sustainable development in agriculture.

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Section: Attempts To Influence Crop Productivitymentioning

confidence: 94%

Section: Attempts To Influence Crop Productivitymentioning

confidence: 99%

Transplastomic plants for innovations in agriculture. A review

Wani

Sah

Sági

et al. 2015

Agron. Sustain. Dev.

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“…SBPase is vital to the Calvin circle in photosynthesis. Previous studies have shown that, in transgenic organisms, overexpression of FBP/SBPase genes enhances CO 2 assimilation rates and increases biomass production (Miyagawa et al, 2001;Yabuta et al, 2008;Ichikawa et al, 2010;Ogawa et al, 2015). Higher expression of SBPase may explain why the sugar contents of tobacco leaves are higher in the Huili region compared to the Miyi region.…”

Section: Degs In Photosynthesismentioning

confidence: 98%

Different tobacco cultivation regions lead to variations in tobacco leaf gene expression profiles involved in carbonhydrate metabolism and ion transportation

Lu¹,

Xu²,

Chen³

et al. 2016

Plant Omics

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Tobacco is an important crop economically in China, and tobacco quality varies across its cultivated regions. To unveil the mechanisms that result in such varied tobacco leaf qualities, we have analyzed the tobacco leaf gene expression profiles of four areas in Sichuan province, China. Tobacco leaves in the 12 th position were collected from plants in four different regions, namely Huili, Miyi, Xingwen and Jiange, and submitted for microarray analysis. Gene expression levels from Miyi group were used as control, and that from Jiange, Xingwen and Huili were compared to Miyi group respectively. A total of 5154 differentially expressed genes (DEGs) were detected, among which 2731 genes were up-regulated and 2423 genes were down-regulated. Based on gene ontology (GO) analysis, significant DEGs were classified into 15 categories (P≤0.05) based on properties pertaining to transferase activity, transmembrane transporter activity, binding, transcription regulator activity, metabolic processes, secondary metabolic processes, etc. Our results also show that these DEGs were significantly enriched in 11 pathways, including metabolic pathways, starch and sucrose metabolism, photosynthesis, etc. Our study suggests that many of the genes involved in various plant physiological and biochemical processes significantly differed across these four different regions, which may explain their differences in tobacco leaf quality.

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“…We also succeeded in the generation of transplastomic lettuce plants carrying the cyanobacterial fbp/sbp transgene with approximately 14-fold higher levels of activity of FBPase compared with the control plants. 55) The photosynthetic capacity and productivity of transplastomic lettuce were increased by 1.3-fold and 1.6-fold, respectively. By analyzing transgenic and transplastomic plants, an increase of just 2-to 3-fold in FBPase activity was found to be sufficient to provide a positive effect on photosynthetic activity with their early maturation or higher yields.…”

Section: Enhancement In Plant Productivity By Improved Photosyntmentioning

confidence: 98%

Diversity of regulatory mechanisms of photosynthetic carbon metabolism in plants and algae

Tamoi

Shigeoka

2015

Bioscience, Biotechnology, and Biochemistry

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To clarify the regulatory mechanisms of the Calvin cycle in algae, we analyzed the molecular properties of the enzymes involved in this cycle. We demonstrated that these enzymes were not regulated by redox modulation through the ferredoxin/thioredoxin system under light/dark conditions and were not sensitive to treatments with hydrogen peroxide in vitro, unlike the chloroplastic thiol-modulated enzymes of plants. On the other hand, we found that cyanobacteria possessed a unique enzyme involved in the Calvin cycle. The CP12 protein played an important role in regulating carbon metabolism in the Calvin cycle in cyanobacteria and eukaryotic algae. This review described the regulatory mechanisms of the Calvin cycle in algae and also the effects of alterations to photosynthetic carbon metabolism on plant productivity, carbon partitioning, and the carbon/nitrogen balance using transgenic plants expressing algal genes.

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Generation of transplastomic lettuce with enhanced growth and high yield

Cited by 41 publications

References 18 publications

Transplastomic plants for innovations in agriculture. A review

Transplastomic plants for innovations in agriculture. A review

Different tobacco cultivation regions lead to variations in tobacco leaf gene expression profiles involved in carbonhydrate metabolism and ion transportation

Diversity of regulatory mechanisms of photosynthetic carbon metabolism in plants and algae

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