Genes and Gene Products Involved in the Synthesis of F-Pili

Laine, Susan; Moore, Deanna; Kathir, Pushpa; Ippen‐Ihler, Karin

doi:10.1007/978-1-4613-2447-8_38

Cited by 24 publications

(42 citation statements)

References 17 publications

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“…There appears to be a pool of F-pilin in the outer membrane as well. Ippen-Ihler and colleagues first pointed out that F-pilin fractionating with the outer membrane was greatly reduced or not present in cells with tra mutations that did not affect accumulation of inner membrane F-pilin (Laine et al, 1995). Their observations suggested that inner-and outer membrane F-pilin are not merely the result of cross-contamination of membrane fractions.…”

Section: F-pilin In the Cell Envelopementioning

confidence: 99%

Towards a structural biology of bacterial conjugation

Silverman

1997

Molecular Microbiology

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SummaryHorizontal DNA transfer among bacteria (conjugation) requires the formation of secure intercellular contacts before DNA transfer can occur. The formation of such contacts among Gram-negative bacteria is mediated by conjugative pili. Like other pili, conjugative pili are filaments extending from the surface of donor cells. They are composed, in so far as is known, entirely of conjugative pilin subunits. Here, we review the structure of F-pilin, the subunit of which F-pili are composed. We emphasize recent studies suggesting a specific domain organization for F-pilin and present a model of how these domains might be arranged in filament subunits.

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Section: F-pilin In the Cell Envelopementioning

confidence: 99%

Towards a structural biology of bacterial conjugation

Silverman

1997

Molecular Microbiology

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“…Subsequent studies (18,23) showed that if traA was expressed in hosts lacking traQ, the 13-kDa pilin precursor protein was the major traA product; a minor 7-kDa product was seen only when traA was expressed at high levels. In TraQ+ cells, however, the 13-kDa traA product was observed only under conditions of overproduction.…”

mentioning

confidence: 99%

“…In TraQ+ cells, however, the 13-kDa traA product was observed only under conditions of overproduction. Instead, the major traA product was a 7-kDa polypeptide [named traA product 7(Q) or Ap7(Q)] that was expressed very efficiently (23). Laine et al also detected small amounts of an 8-kDa traA product (Ap8) (23 F pili or detectable quantities of F-pilin subunits (22).…”

mentioning

confidence: 99%

“…Instead, the major traA product was a 7-kDa polypeptide [named traA product 7(Q) or Ap7(Q)] that was expressed very efficiently (23). Laine et al also detected small amounts of an 8-kDa traA product (Ap8) (23 F pili or detectable quantities of F-pilin subunits (22). Thus, the traQ product, a 94-amino-acid inner membrane protein (48,49), appears to be required for efficient utilization and processing of the pilin precursor.…”

mentioning

confidence: 99%

“…Thus, the traQ product, a 94-amino-acid inner membrane protein (48,49), appears to be required for efficient utilization and processing of the pilin precursor. It was suggested that Ap8 might be an intermediate in such processing (23).…”

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confidence: 99%

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The Escherichia coli K-12 F plasmid gene traX is required for acetylation of F pilin

et al. 1993

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The Escherichia coli F plasmid gene required for amino-terminal acetylation of F-pilin subunits was identified. Using Western blots (immunoblots), we assayed the reaction of monoclonal antibodies with F-pilin polypeptides in inner membrane preparations from various F mutant strains. It was known that JEL92 recognizes an internal pilin epitope and JEL93 recognizes the acetylated amino-terminal sequence (L.S. Frost, J.S. Lee, D.G. Scraba, and W. Paranchych, J. Bacteriol. 168:192-198, 1986). As expected, neither antibody reacted with inner membranes from F- cells or Flac derivatives that do not synthesize pilin. Mutations that affected the individual activities of F tra genes traA, -B, -C, -D, -E, -F, -G, -H, -I, -J, -K, -L, -M, -N, -P, -R, -U, -V and -W or trb genes trbA, -B, -C, -D, -E, -G, -H, and -I did not prevent JEL92 or JEL93 recognition of membrane pilin. However, Hfr deletion mutants that lacked the most-distal transfer region genes did not express pilin that reacted with JEL93. Nevertheless, all strains that retained traA and traQ did express JEL92-reactive pilin polypeptides. Analysis of strains expressing cloned tra segments showed that traA and traQ suffice for synthesis of JEL92-reactive pilin, but synthesis of JEL93-reactive pilin is additionally dependent on traX. We concluded that the traX product is required for acetylation of F pilin. Interestingly, our data also showed that TraA+ TraQ+ cells synthesize two forms of pilin which migrate at approximately 7 and 8 kDa. In TraX+ cells, both become acetylated and react with JEL93. Preparations of wild-type F-pilus filaments contain both types of subunits.

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Genetic Organization of Transfer-Related Determinants on the Sex Factor F and Related Plasmids

Ippen‐Ihler

Skurray

1993

Bacterial Conjugation

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Genes and Gene Products Involved in the Synthesis of F-Pili

Cited by 24 publications

References 17 publications

Towards a structural biology of bacterial conjugation

Towards a structural biology of bacterial conjugation

The Escherichia coli K-12 F plasmid gene traX is required for acetylation of F pilin

Genetic Organization of Transfer-Related Determinants on the Sex Factor F and Related Plasmids

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